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Trichosporon asahii 2-1 and application thereof in preparing acrylic acid

A technology of Trichosporium and Trichosporium, applied in the application field of preparing acrylic acid, can solve the problems of easy pollution, high reaction energy consumption and high equipment requirements

Active Publication Date: 2010-07-07
ZHEJIANG UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The yield of acrylic acid in this reaction process is relatively high, and the single-pass yield can reach 85%, but the reaction has high energy consumption, high equipment requirements, and is easy to pollute
[0006] The currently reported biological production methods mainly include lactic acid hydrolysis and acrylonitrile hydrolysis, but the strains used are mainly bacteria, and the research on producing acrylic acid with yeast amidase has not been reported.

Method used

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  • Trichosporon asahii 2-1 and application thereof in preparing acrylic acid
  • Trichosporon asahii 2-1 and application thereof in preparing acrylic acid

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0074] Seed medium formula: 1.0g acrylic acid, 15.0g sucrose, 5.0g glucose, 10g yeast extract, 5g NaCl, KH 2 PO 4 1.0g, MgSO 4 .7H 2 O 0.2g, FeSO 4 0.01g, prepared with deionized water, adjusted the pH to 7.0 with ammonia water, the total volume is 1L.

[0075] Fermentation medium: acrylic acid 10.0g, sucrose 20.0g, yeast extract 5.0g, NaCl 1.0g, KH 2 PO 4 1.0g, MgSO4.7H 2 O 0.2g, FeSO 4 0.1g, prepared with deionized water, adjusted the pH to 7.0 with ammonia water, the total volume is 1L.

[0076] Take 120mL of the above-mentioned seed culture medium, evenly distribute it in four 250mL Erlenmeyer flasks, and sterilize. Insert Trichosporon assahii 2-1, a slant fungus, at a shaker speed of 150r / min, and culture on a shaker at 30°C for 20h as a seed solution for later use.

[0077] Take 1L of the above-mentioned fermentation medium, evenly distribute it into ten 500mL shake flasks, and sterilize. The seed liquid was inserted, the inoculum amount was 2% (V / V), and c...

Embodiment 2

[0080] The composition of the seed medium and the cultivation of the seeds are the same as in Example 1.

[0081] Fermentation enzyme production medium formula: acrylic acid 5.0g, sucrose 15.0g, yeast extract 5.0g, urea 1.0g, NaCl 1.0g, KH 2 PO 4 0.5g, MgSO 4 .7H 2 O 0.5g, FeSO 4 0.1g, prepared with deionized water, adjusted the pH to 7.0 with ammonia water, the total volume is 1L;

[0082] Take 1L of the above-mentioned fermentation medium, evenly distribute it into ten 500mL shake flasks, and sterilize. The seed liquid was inserted, the inoculum amount was 2% (V / V), and culture was carried out, the culture temperature was 30° C., the culture time was 20 h, and the rotation speed of the shaker was 150 r / min.

[0083] After the cultivation was completed, the fermentation broth was centrifuged at 5000 r / min at 15° C. for 5 minutes to collect the bacterial cells, and the bacterial cells were suspended in phosphate buffer for transformation reaction. The reaction system i...

Embodiment 3

[0085] The composition of the seed medium and the cultivation of the seeds are the same as in Example 1.

[0086] Fermentation enzyme production medium formula: 3.0g acrylic acid, 15g sucrose, 5.0g yeast extract, 2.0g urea, 1.0g NaCl, KH 2 PO 4 0.5g, MgSO 4 .7H 2 O 0.5g, FeSO 4 0.2g, prepared with deionized water, adjusted the pH to 8.0 with ammonia water, the total volume is 1L.

[0087] Take 1L of the above-mentioned fermentation medium, evenly distribute it into ten 500mL shake flasks, and sterilize. The seed liquid was inserted, the inoculum amount was 2% (V / V), and culture was carried out, the culture temperature was 30° C., the culture time was 20 h, and the rotation speed of the shaker was 150 r / min.

[0088] After the cultivation is completed, the fermentation broth is centrifuged at 5000 r / min at 15° C. for 5 minutes to collect the bacterial cells, and the bacterial cells are suspended in the buffer for transformation reaction. The reaction system is 100mL, in...

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Abstract

The invention provides an acrylic amidase strain-Trichosporon asahii 2-1 and application thereof in preparing an acrylic acid. The Trichosporon asahii 2-1 is collected in the China center for type culture collection of which the address is Mountain Luojia Wuhan University in Wuhan City, Hubei Province, 430072, with a collection date of January 19, 2010, and a collection number CCTCC No. is M2010017. The invention obtains a new strain producing amidase through screening and a mutagenesis strain with obviously enhanced amidase activity through mutagenesis improvement, prepares the acrylic acid by a biological method with the mutagenesis strain so as to replace a chemical synthesis method, and has the advantages of high conversion rate, environmental protection, green process, and the like. meanwhile, the acrylic amidase strain provides a most economic and effective method for treating aliphatic nitrile and amide compounds contained in industrial waste water because the strain can hydrolyze a highly virulent aliphatic nitrile compound and acrylic amide into acid substances with less virulence, thereby also having important practical significance in environmental improvement.

Description

(1) Technical field [0001] The invention relates to an acrylamidase bacterial strain-Trichosporon asahii 2-1, and its application in the preparation of acrylic acid. (2) Background technology [0002] Trichosporon asahii (Trichosporon asahii) belongs to the phylum Deuteromycota, class Bacillus, order Cryptococcus, family Cryptococcus, and genus Thygomyces. [0003] Amidase (amidase, EC 3.5.1.4) can hydrolyze amides into corresponding carboxylic acids and ammonia. Acrylamidase catalyzes the hydrolysis of acrylamide to acrylic acid and ammonia. Acrylic (C 3 h 4 o 2 ) is a colorless liquid with a pungent odor. It has high polymerization performance and can be homopolymerized by itself, but also can be combined with other vinyl monomers such as phenylacetic acid, vinyl acetate, methyl methacrylate, acrylonitrile, Acrylamide, vinyl chloride and other copolymers can be made into acrylic resin or polyacrylate series products with different properties. These products are widely...

Claims

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Application Information

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IPC IPC(8): C12N1/16C12N9/80C12P7/40C12R1/645
Inventor 张正波高靓裘娟萍赵春田
Owner ZHEJIANG UNIV OF TECH
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