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Application of jerusalem artichoke extract in preparing anti-tumor medicine or anti-tumor health care product

A technology of anti-tumor drugs and extracts, applied in the field of application of Jerusalem artichoke leaf extracts in the preparation of anti-tumor drugs, to achieve the effects of being beneficial to environmental and ecological governance, increasing farmers' income, and enriching natural drug resources

Inactive Publication Date: 2010-06-23
DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are almost no reports on the pharmacological activity of Jerusalem artichoke leaves.

Method used

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  • Application of jerusalem artichoke extract in preparing anti-tumor medicine or anti-tumor health care product
  • Application of jerusalem artichoke extract in preparing anti-tumor medicine or anti-tumor health care product
  • Application of jerusalem artichoke extract in preparing anti-tumor medicine or anti-tumor health care product

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Example 1 The inhibitory effect of Jerusalem artichoke leaf ethyl acetate extract on small cell lung cancer cell line A549

[0022] Preparation of Jerusalem artichoke leaf ethyl acetate extract: dry and crush the Jerusalem artichoke leaf, weigh it quantitatively, mix the Jerusalem artichoke leaf with ethyl acetate at a ratio of 1g:10ml, soak for 24 hours, filter, and concentrate under reduced pressure to extract; During the experiment, the required concentration was prepared with complete medium.

[0023] Small cell lung cancer cell line A549, with containing 10% newborn bovine serum (volume ratio), the sodium pyruvate of 1.0mM, penicillin 100,000 units / liter, the MEM (Eagle) medium of streptomycin 100,000 μg / liter at 37 °C, 5% CO 2 Cultivate in the incubator of (volume ratio); During the experiment, get the logarithmic growth phase cell, digest with 0.25% trypsin digestion solution, adjust the cell concentration to be 10 5 1 / mL, add 100 μL / well in 96-well plate, and ...

Embodiment 2

[0027] Example 2 The inhibitory effect of Jerusalem artichoke leaf ethyl acetate extract on breast cancer cell line MCF-7

[0028] The preparation of the ethyl acetate extract of Jerusalem artichoke leaves is the same as in Example 1. When doing pharmacological experiments, prepare the required concentration with complete medium.

[0029] Breast cancer cell line MCF-7 was cultivated in an incubator at 37°C and 5% CO2 with RPMI1640 medium containing 10% newborn calf serum, 100,000 units / liter of penicillin, and 100,000 μg / liter of streptomycin; Take cells in the logarithmic growth phase, digest with 0.25% trypsin digestion solution, and adjust the cell concentration to 10 5 1 / mL, add 100 μL / well in 96-well plate, and cultivate overnight; add 100 μL of ethyl acetate extracts of Jerusalem artichoke leaves prepared in four different concentrations to each well, and continue to cultivate for 24 hours. The growth inhibition rate was detected by MTT method, and the IC was calculated...

Embodiment 3

[0032] Example 3 The inhibitory effect of Jerusalem artichoke leaf ethyl acetate extract on cervical cancer cell line Hela

[0033] The preparation of the ethyl acetate extract of Jerusalem artichoke leaves is the same as in Example 1. When doing pharmacological experiments, prepare the required concentration with complete medium.

[0034] Cervical cancer cell line Hela was cultured in RPMI1640 medium containing 10% newborn bovine serum, 100,000 units / liter of penicillin, and 100,000 μg / liter of streptomycin in an incubator at 37°C and 5% CO2; Count the cells in the growth phase, digest with 0.25% trypsin digestion solution, and adjust the cell concentration to 10 5 1 / mL, add 100 μL / well in 96-well plate, and cultivate overnight; add 100 μL of ethyl acetate extracts of Jerusalem artichoke leaves prepared in four different concentrations to each well, and continue to cultivate for 24 hours. The growth inhibition rate was detected by MTT method, and the IC was calculated. 50 v...

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PUM

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Abstract

The invention relates to anti-tumor medicine, in particular to the application of an organic solvent extract of jerusalem artichoke in preparing the anti-tumor medicine or an anti-tumor health care product. An external cell experiment shows that an ethyl acetate extract in a jerusalem artichoke leaf has the inhibiting effect for diversified tumor cells such as lung cancer cell strains (A549), breast cancer cell strains (MCF-7), cervical cancer cell strains (Hela) and kidney cancer cell strains (ACHN).

Description

technical field [0001] The invention relates to antitumor drugs, in particular to the new application of Jerusalem artichoke, a plant of the genus Asteraceae, in pharmacy, especially the application of Jerusalem artichoke leaf extract in the preparation of antitumor drugs. Background technique [0002] Jerusalem artichoke (Helianthus tuberosus Linn), commonly known as Jerusalem artichoke and ghost ginger, is native to North America and is a perennial herb of the genus Helianthus L. in the family Compositae. It has the characteristics of strong reproductive ability, water and soil conservation, and extensive management. It is currently planted in desert areas such as Northwest and Northeast my country, and has a good effect of windbreak and sand fixation. [0003] Foreign research on the development and utilization of Jerusalem artichoke began in the 1970s, mainly focusing on the development and utilization of Jerusalem artichoke tubers, studying the extraction and utilization...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/28A61P35/00A61K135/00A61K133/00A61K125/00
Inventor 肖红斌程孟春袁晓艳杜昱光程受衍
Owner DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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