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Medical application of 6-shogaol for treating cervical cancer, leukemia and breast cancer

A technology of shogaol and cervical cancer, which is applied in the direction of medical preparations containing active ingredients, pharmaceutical formulas, antineoplastic drugs, etc., and can solve the problems of weak activity and weak activity

Active Publication Date: 2010-06-09
CHINA PHARM UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Recently, foreign literature reported that 6-shogaol has antitumor activity on human lung cancer A549 cells, [6-Shogaol, an Active Constituent of DietaryGinger, Induces Autophagy by Inhibiting the AKT / mTOR Pathway in Human Non-SmallCell Lung Cancer A549 Cells .J Agric Food Chem.2009 Oct 2.], but its activity is not strong, and the IC50 of inhibiting the growth of A549 cells is 55.4μM; there are also literature reports that 6-shogaol has activity on human colon cancer COLO 205 cells [6-Shogaol induces apoptosis in human colorectal carcinoma cells via ROS production, caspase activation, and GADD 153 expression. Mol Nutr Food Res.2008, 52, 527-37.], its activity is also very weak, and the IC50 of inhibiting the growth of COLO 205 cells > 60μM

Method used

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  • Medical application of 6-shogaol for treating cervical cancer, leukemia and breast cancer
  • Medical application of 6-shogaol for treating cervical cancer, leukemia and breast cancer
  • Medical application of 6-shogaol for treating cervical cancer, leukemia and breast cancer

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0012] Antitumor effect of 6-shogaol in vitro

[0013] Take the cells in the logarithmic growth phase and inoculate 4~10×10 cells according to the size of the cells 3 Each was placed on a 96-well plate, and after 24 hours of growth, the supernatant was discarded, and then administered according to the following groups: Tumor cells were set up with no drug group and drug-added group (concentration 1-25 μM), and each group was set up with 5 or 6 replicates. Incubate wells for 24 or 72 hours, discard the supernatant, add 100 μl of MTT (tetrazolium salt) serum-free medium containing 0.5 mg / ml and incubate for 4 hours, add 100 μl DMSO (dimethyl sulfoxide), and place in a micro shaker Shake it for 10 minutes, and then place it on a microplate reader to detect the OD value at 570nm. Normal human cell line L-O2 was used for toxicity evaluation, and each experiment was repeated 3 times. The results are shown in Table 1 and Figure 1 ~ Figure 2 .

[0014] Table 1 IC of 6-shogaol on ...

Embodiment 2

[0020] Cell morphology observation and AO (acridine orange) staining

[0021] Tumor cells were divided into no-drug group and drug-added group (5μM). Cells were treated for different time (0, 2, 4, 8, 12, 24 hours), fixed with 4% paraformaldehyde for 30 minutes, washed 2-3 times with 0.01M PBS, stained with AO for 10 minutes, and observed the morphology of the nucleus under a fluorescent microscope .

[0022] It was found that the morphology of the cells in the negative treatment group was normal, and the nuclei were intact after staining with the staining reagent. Many cells were rounded or shrunk after being treated with 6-shogaol, and the nuclei were found to be pyknotic when excited by blue light under a fluorescent microscope.

[0023] After AO fluorescent staining, the nuclei of typical apoptotic cells were densely stained and showed blocky or granular yellow-green fluorescence; normal cells were evenly stained; dead cells were not stained. The experimental results sho...

Embodiment 3

[0025] Detection of cell apoptosis by flow cytometry

[0026] In the experiment, a negative control group (ie no drug treatment group) and a 6-shogaol treatment group (15 μM) were set up. After the cells were treated with 6-shogaol for 12 hours, they were digested with 0.1% trypsin without EDTA. After the digestion was terminated by the serum-containing medium, the cells were collected in a 10ml glass centrifuge tube, centrifuged at 500rpm (or 100g) for 5min, discarded After washing with 0.01M PBS at 500rpm (or 100g) for 5min, centrifuge twice, then add the binding liquid according to the instructions of the Annexin V / PI double staining kit and perform staining for flow cytometry (Becton Dickinson FACScan Flow Cytometer) detection. FACS data were analyzed by professional flow cytometer operators using FLOWJO software (Tree Star, CA). The results showed that 6-shogaol could induce apoptosis.

[0027] The 18-hour apoptosis rate of the negative group without drugs was 4.8±1.7%....

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Abstract

The invention relates to the field of natural medicines, in particular to application of 6-shogaol to treating cervical cancer, leukemia and breast cancer. A pharmacological activity research of the 6-chogaol discovers that even if the 6-shogol displays the curative activity to a plurality of tumors, the activity differences of different tumors are great, the 6-shogaol has best treating effects to cervical cancer, leukemia and breast cancer in massive tumor inhabitant experiments, and the treating effects to the three cancers are remarkably better than that of other cancers.

Description

technical field [0001] The invention relates to the field of natural medicines, in particular to the use of 6-shogaol in the treatment of cervical cancer, leukemia and breast cancer. Background technique [0002] Ginger and dried ginger are the fresh rhizome and dried rhizome of Zingiber officinale Rosc., which are commonly used in traditional Chinese medicine. The clinical application of ginger is quite extensive, and foreign countries pay more attention to it. In recent years, there are abundant chemical and pharmacological reports on ginger and its main active ingredients. 6-shogaol (6-shogaol) is one of the volatile components in ginger, and its molecular formula is: C 17 h 24 o 3 , molecular weight: 276.37, yellow powder or crystal, easily soluble in ethanol, insoluble in water, its chemical structure is as follows: [0003] [0004] It has been reported in the literature that 6-shogaol has biological activities such as cardiotonic, antiplatelet or antithromboti...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/12A61P35/00A61P35/02
CPCA61K31/122A61P35/00A61P35/02
Inventor 齐炼文李萍刘慧成小兰彭咏波
Owner CHINA PHARM UNIV
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