Micro optical fiber biosensor for detecting nitric oxide concentration in organism

A biosensor, nitric oxide technology, applied in the field of biological detection and clinical medical detection, can solve the problems of difficult optical fiber processing, delicate structure, hard silica material, etc. short time effect

Inactive Publication Date: 2010-05-19
WUHAN UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Because the optical fiber is very thin (the core diameter is generally 5-9 μm, and the cladding diameter is generally 125 μm), and the silica material that makes u...

Method used

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  • Micro optical fiber biosensor for detecting nitric oxide concentration in organism
  • Micro optical fiber biosensor for detecting nitric oxide concentration in organism
  • Micro optical fiber biosensor for detecting nitric oxide concentration in organism

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] 1) Using a 157nm deep ultraviolet laser direct etching process to etch microgrooves on the multimode silica fiber, the etched microgrooves have a width of 20-40 μm and a groove length of about 100-120 μm. A Ag film is deposited on the end face of the fiber optic probe to form a reflective film by vacuum evaporation.

[0025] 2) Weigh 0.1g of cellulose acetate and dissolve it in 3.5mL of acetone, seal and stir for 2h, then add 0.25mL of diaminofluoresceins (DAFs) with a concentration of 0.5mmol / ml and 0.5ml of double distilled water to the mixture, and then seal Stir for 6 hours; pour the evenly stirred mixture into a glass biochemical culture dish with a diameter of Φ60 mm, seal the dish with plastic wrap, make holes of appropriate size and number on the plastic wrap, and place it on a horizontal platform at room temperature until the acetone is completely Volatile film can be.

[0026] 3) The NO-sensitive membrane prepared in step 2) is fixed in the optical fiber micr...

Embodiment 2

[0029] Except for replacing the NO fluorescent indicator, other implementation methods are the same as in Example 1.

[0030]Preparation of NO sensitive film: Weigh 0.1g of cellulose acetate and dissolve it in 3.5mL of acetone, and stir for 2h in a closed manner, then add 0.25mL of diaminorhodamines (DARs) with a concentration of 0.5mmol / ml and 0.5ml of double-distilled water, and then airtightly stir for 6 hours; pour the evenly stirred mixture into a glass biochemical culture dish with a diameter of Φ60mm, seal the dish with plastic wrap, make holes of appropriate size and number on the plastic wrap, and place it on a horizontal platform at room temperature Up until the acetone is completely volatilized to form a film.

Embodiment 3

[0032] 1) Using a 157nm deep ultraviolet laser direct etching process to etch microgrooves on the multimode silica optical fiber, the width of the etched microgrooves is about 20-40 μm, and the groove length is about 100-120 μm. The end face of the optical fiber probe is coated with Ag film by vacuum evaporation method to form a reflective film.

[0033] 2) Weigh 0.1g of cellulose acetate and dissolve it in 3.5mL of acetone, seal and stir for 2h, then add 0.25mL of diaminofluoresceins (DAFs) with a concentration of 0.5mmol / ml and 0.5ml of double distilled water to the mixture, and then seal Stir for 6 hours; absorb the evenly stirred mixture and drop it directly into the microgroove of the optical fiber probe described in 1), seal the probe with plastic wrap, and place it on a horizontal platform at room temperature until the acetone is completely volatilized to form a film.

[0034] 3) The optical fiber probe is packaged with a metal sleeve, and the micro-groove structure of ...

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PUM

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Abstract

The invention relates to a micro optical fiber biosensor for detecting nitric oxide concentration in an organism, which comprises a light source, an input optical fiber, a NO optical fiber fluorescent micro probe, an output optical fiber and an information acquiring and data processing system. The micro probe of the sensor is manufactured by processing multi-mode silica optical fibers by using 157nm ultraviolet short pulsed laser technology, and a NO sensitive film is prepared from a NO fluorescent indicator and a high molecular base material. Fixed on the optical fiber micro probe by a physical adsorption method, the sensitive material can be reacted with the NO in the environment to obviously change fluorescence strength signals, so that the detection of the NO concentration can be performed. The detection system of the sensor adopts a reflection type light path design, is quick and convenient in use, and has high sensitivity and precision and good anti-interference performance and stability.

Description

technical field [0001] The invention belongs to the technical field of biological detection and clinical medical detection, and relates to a miniature optical fiber biosensor for detecting NO concentration in living organisms. Background technique [0002] Nitric oxide (NO) is an extremely unstable biological free radical with a small molecule and simple structure, and its biological half-life is only 3 to 5 seconds. It has been found that NO is an important messenger molecule and effector molecule in organisms, and has an important regulatory effect on the cardiovascular and cerebrovascular systems, digestive system, and nervous system. Its detection is widely used in the fields of life sciences, basic medicine, and clinical medicine. are very important. [0003] At present, the methods for detecting NO mainly include electrochemical method, chemiluminescence method, instrumental analysis method, isotope radiation intensity method, indirect detection method, etc. It satis...

Claims

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Application Information

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IPC IPC(8): G01N21/64G03F7/00
Inventor 丁莉芸黄俊戴玉堂黄兰芬王海钟韵鸣
Owner WUHAN UNIV OF TECH
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