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Method for improving transplant survival rate of test tube dendrobium devonianum seedlings by resistance processing

A technology of transplanting survival rate and dendrobium dendrobium, applied in horticultural methods, botanical equipment and methods, horticulture, etc., can solve problems such as unsatisfactory effects, achieve simple resistance treatment process, promote fibrosis, and reduce growth metabolism speed effect

Inactive Publication Date: 2010-04-21
CHONGQING ACAD OF CHINESE MATERIA MEDICA
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Problems solved by technology

Chinese patent application (200810195801.2) discloses a kind of seedling hardening method of Dendrobium candidum tissue culture seedling, and it is mainly when the bottle seedling of tissue culture respectively when bottle cap is sealed, when cap is loosened, uncaps, respectively light from if to strong Seedling hardening; and when transplanting, the light is from weak to strong, and the humidity is from high to low to harden the seedlings; the above-mentioned patent expects to achieve the purpose of improving the resistance of Dendrobium officinale by hardening the seedlings; but the effect is not ideal

Method used

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Embodiment Construction

[0014] Preparation of proliferation medium: Add 1 mg of NAA to 1 L of KC to make a solid medium.

[0015] Preparation of rooting medium: add banana extract and sucrose to MS, the concentration of banana extract is 15-30%, the concentration of sucrose is 1.5-3%; add NAA0.1-1.0mg, agar 2-4g per L medium, Adjust the pH to 5.6-6.0 to make root culture medium.

[0016] Seedbed preparation: Use wood-processed scraps or asbestos tiles as the bottom to make a planting bed with a bed height of 10-15 cm, a bed width of 1-1.2 meters, a bed length depending on the site, and a support of 0.8-1.0 meters. Use broken bark as a substrate to spread on the seedbed with a thickness of 5-10 cm. Broken bark is preferably sterilized with high temperature or drugs before use.

[0017] The collection and sterilization of explants, its operation steps are:

[0018] 1) Choose robust new shoots with a length of 3 cm to 6 cm as explants.

[0019] 2) Cut the sprouts from the parent with a sterilized sh...

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Abstract

The invention discloses a method for improving the survival rate of transplanted test tube dendrobium devonianum seedlings by resistance processing, which comprises the steps of early-stage cultivation, resistance processing, late-stage cultivation and seedling transplant and has the advantages that: the plants are placed under an extreme temperature condition to reduce the growth and metabolism speed of the plants to promote the fibration of stems and strengthen the environmental adaptability of the plants; and in an artificial adverse environment, the stems and twigs of the plants expand quickly, leaves fall and resistance increases, and thus the transplant survival rate is improved. The method is a simple resistance processing process with high maneuverability and low cost.

Description

technical field [0001] The invention relates to a method for improving the transplanting survival rate of dendrobium dendrobium, in particular to a method for improving the transplanting survival rate of dendrobium dendrobium test-tube seedlings through resistance treatment. technical background [0002] Dendrobium is the second largest genus of Orchidaceae. There are about 1,500 species in the world. There are 74 species and 2 varieties in my country, of which more than 40 are available for medicinal use, mainly distributed in South China and Southwest China. Among them, there are mainly Dendrobium officinale, Dendrobium purple, Dendrobium nobile, Dendrobium dendrobii, Dendrobium huoshanense, Dendrobium dendrobii; Dendrobium is a famous and precious Chinese medicinal material, which has a long history in my country. Listed as the top grade, "sweet, flat. In the main injury, eliminate numbness, lower qi, nourish the five viscera, win thinness, strengthen yin. Long-term use th...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 蒋渝明兴加钟国跃秦松云王昌华孙年喜
Owner CHONGQING ACAD OF CHINESE MATERIA MEDICA
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