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Radionuclide-labelled biodegradable bioabsorbable biopolymer nano fibrous membrane, preparation process and application thereof

A biopolymer and nanofiber membrane technology, applied in radioactive carriers, fiber treatment, fiber chemical characteristics, etc., can solve the problem of killing tumor cells but rarely reported in the literature

Inactive Publication Date: 2010-04-14
INST OF CHEM CHINESE ACAD OF SCI +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Therefore, if the radionuclide and electrospun nanofibrous membrane are combined, after surgical resection of the tumor, implanting the surgical site can not only effectively kill the remaining tumor cells, but also prevent adhesion and promote wound healing. However, the use of radionuclides and degradable electrospun nanofibrous membranes for short-range tumor cell killing has rarely been reported in the literature (Chinese patent application number: 200710099007.3)

Method used

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  • Radionuclide-labelled biodegradable bioabsorbable biopolymer nano fibrous membrane, preparation process and application thereof
  • Radionuclide-labelled biodegradable bioabsorbable biopolymer nano fibrous membrane, preparation process and application thereof
  • Radionuclide-labelled biodegradable bioabsorbable biopolymer nano fibrous membrane, preparation process and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0050] (1) Preparation of solution: PLGA (LA / GA=50 / 50 (molar ratio), molecular weight 100,000) is dissolved in N, N dimethylformamide (DMF) and acetone (volume ratio is 5 / 5) In 10 ml of the mixed solvent, a PLGA solution with a concentration of 20 wt % was obtained, and the obtained PLGA solution was placed in a feeding syringe of an electrospinning device.

[0051] (2) Adjust the electrospinning device: select the double spinneret device connected with the injector, and the rotating drum is used as a collector; adjust the distance between the double spinneret and the rotating drum to be 12cm; the ambient temperature of spinning is 25 ℃, The air velocity in the environment is controlled at 0.5-0.8m 3 / hr; Turn on the high-voltage power supply and the feeding syringe pump, adjust the voltage to 20KV, and the feeding speed of the solution is 20ul / min for spinning, and obtain the PLGA nanofiber film on the rotating drum.

[0052] (3) After washing the collected PLGA nanofiber me...

Embodiment 2

[0058] (1) Utilize the PLGA nanofiber membrane prepared in embodiment 1.

[0059] (2) Cut the dried electrospun PLGA nanofiber membrane into 2.25cm 2 Place the small piece of NaOH solution with a concentration of 0.075M in 10ml, react at 0°C for 30 minutes, soak and rinse with deionized water repeatedly, and place it in 5ml containing 50mM EDC and NHS (molar ratio: 1:1). After the activation reaction at 4°C for 16 hours, rinse with deionized water, then put it into 5ml of gelatin aqueous solution containing 10mg / ml, carry out grafting reaction at 30°C for 8 hours, then soak and rinse with a large amount of deionized water . A biodegradable and bioabsorbable PLGA nanofiber membrane grafted with gelatin on the surface is obtained. Such as image 3 As shown, the morphology of the fibers after grafting GE remained good and was not significantly damaged.

[0060] (3) Place the biodegradable and bioabsorbable PLGA nanofibrous membranes grafted with gelatin on the obtained surfac...

Embodiment 3

[0064] (1) Preparation of solution: PCL (molecular weight 100,000) is dissolved in 10ml of a mixed solvent of DMF and acetone (volume ratio is 8 / 2), to obtain a PCL solution with a concentration of 20wt%, and the obtained PCL solution is placed in an electrostatic In the feeding syringe of the spinning equipment.

[0065] (2) The preparation process of electrospinning is the same as that in Example 1.

[0066] (3) After washing the collected PCL nanofiber membrane with deionized water repeatedly, after vacuum drying in a vacuum oven at 20°C for 5 hours, the biodegradable and absorbable PLGA nanofiber membrane material was obtained, and the thickness of the membrane was 104 Micron and nanofibers are arranged in a non-woven fabric structure, and the diameter of the nanofibers is between 500nm and 1 μm.

[0067] (4) Cut the dried electrospun PCL nanofiber membrane into 2.25cm 2 Put the small piece of NaOH solution with a concentration of 0.2M in 10ml, react at 0°C for 10 minute...

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Abstract

The invention relates to a radionuclide-labelled biodegradable bioabsorbable biopolymer nano fibrous membrane, a preparation process and application thereof. The preparation process includes steps of firstly adopting the electrostatic spinning technique to prepare a biopolymer nano fibrous membrane consisting of biopolymer nano fibers with diameters ranging from 50nm to 5000nm, performing chemical modification on the surface of the biopolymer nano fibrous membrane, enabling the surface of the biopolymer nano fibrous membrane to be in coupling reaction with double-functional-group coupling agent, chelating the surface of the biopolymer nano fibrous membrane after coupling reaction in buffer solution of acetic acid-sodium acetate anhydrous containing radionuclide compounds, and finally obtaining the radionuclide-labelled biodegradable bioabsorbable biopolymer nano fibrous membrane, wherein the double-functional-group coupling agent which is combined with amino in biopolymer nano fibers via chemical bonds on the surface of the biopolymer nano fibrous membrane, the radionuclide is chelated and fixed on the surface of the biopolymer nano fibrous surface via the double-functional-group coupling agent. The radionuclide-labelled biodegradable bioabsorbable biopolymer nano fibrous membrane can be used as an oncotherapy material or a material for focal tissue portions after tumor resection operation.

Description

technical field [0001] The invention belongs to the field of polymer nanofiber membranes containing radionuclide, and in particular relates to biodegradable and bioabsorbable biopolymer nanofiber membranes labeled with radionuclides, a preparation method and medical application thereof. Background technique [0002] Malignant tumors have become the main cause of human death and seriously endanger people's health. Tumor targeted therapy is one of the biological treatment methods for malignant tumors. Its principle is to use highly specific pro-tumor substances as carriers, and combine cytotoxic substances such as radionuclides, chemotherapeutic drugs, toxins, etc. with the carrier. , relying on the specificity of the carrier and the affinity of the tumor, the cytotoxic substance is concentrated on the tumor site as much as possible to play a stable killing effect, and it is harmless to the normal tissue of the host. Internal radionuclide radiation therapy is a promising tumo...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K9/70A61K51/06A61P35/00A61P41/00A61P17/02A61P7/04D01F6/62D01F6/84D01F6/76D06M15/03D06M15/15D06M15/59D06M10/06D06M10/08D06M13/342A61K103/32A61K103/20A61K103/10A61K101/02
Inventor 韩志超贺爱华聂华荣许杉杉王凡
Owner INST OF CHEM CHINESE ACAD OF SCI
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