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Occult blood detection film and preparation method and application thereof and occult blood detection kit

A detection kit and detection film technology, applied in the field of occult blood detection, can solve the problems of complex detection, high cost and high technical requirements

Pending Publication Date: 2020-10-23
ANKON MEDICAL TECH SHANGHAI CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In the prior art, some researches have been carried out on the test methods of occult blood detection, but there are still more or less defects in the existing occult blood detection, for example, some existing methods of using fecal occult blood test strips for fecal occult blood detection have technical requirements Defects that are relatively high, costly or more complex to detect, need to be further improved

Method used

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  • Occult blood detection film and preparation method and application thereof and occult blood detection kit
  • Occult blood detection film and preparation method and application thereof and occult blood detection kit

Examples

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preparation example Construction

[0087] In a second aspect, in some embodiments, a method for preparing a thin film for detecting occult blood is provided, comprising the following steps:

[0088] Provide polyion film;

[0089] Immersing the polyion film into the dye, so that the dye is attached to the polyion film to obtain the occult blood detection film.

[0090] Wherein, the dye is suitable for reacting with hemoglobin, and the color of the dye is different in environments with different hemoglobin concentrations. The dye includes at least one of methylene blue dye or proanthocyanidin dye.

[0091] The preparation method of the occult blood detection film is a simple, convenient and efficient preparation method, and has the advantages of simple synthesis, easy operation, low technical threshold, easy realization of large-scale production, and the like.

[0092] It should be understood that for the same or similar parts in the preparation method of the blood occult detection film of the second aspect and...

Embodiment 1

[0159] The preparation of occult blood detection film comprises the following steps:

[0160] Take bromobutane and vinylimidazole in an equimolar ratio and put them in a glass bottle, and ultrasonicate for 15 minutes until the two are fully mixed. After removing impurities, add an acrylonitrile solution equal to the mass sum of bromobutane and vinylimidazole, and then add bromobutane, vinylimidazole and acrylonitrile with a mass ratio of 8% The mass ratio of MBA and TPO accounting for 1% of the total mass of bromobutane, vinylimidazole and acrylonitrile is then ultrasonicated for 15 minutes to form a clear and transparent film-forming liquid.

[0161] Prepare a glass plate (10cm×10cm), wet the glass plate with water first, and then stick tin foil on the surface of the glass plate, with the smooth side of the tin foil facing up, wipe it with a dust-free cloth until there are no wrinkles, and apply the second layer on the tin foil. A lubricant white petroleum jelly, continue to...

Embodiment 2

[0165] The preparation of occult blood detection thin film, differs from embodiment 1 only in:

[0166] Will be 200mm 2 The polyion film was immersed in 1 mg / mL methylene blue dye, taken out after 30 minutes at room temperature, and washed with water to obtain an occult blood detection film.

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Abstract

The invention provides an occult blood detection film and a preparation method and application thereof and an occult blood detection kit, and relates to the technical field of occult blood detection.The preparation method of the occult blood detection film comprises the following steps: providing a polyion film; and immersing the polyion film into a dye, so that the dye is attached to the polyionfilm to obtain the occult blood detection film, wherein the dye is suitable for reacting with hemoglobin, the colors of the dye are different in environments with different hemoglobin concentrations,and the dye comprises at least one of methylene blue dye or procyanidine dye. The method has the characteristics of simple operation, strong anti-interference performance, obvious color development change, fast detection and the like, and can alleviate the problems of high technical requirements, high cost, complex detection and the like in occult blood detection in the prior art.

Description

technical field [0001] The invention relates to the technical field of occult blood detection, in particular to a thin film for occult blood detection, its preparation method, application and a kit for occult blood detection. Background technique [0002] Clinically, when a patient bleeds heavily or rapidly from the gastrointestinal tract, the doctor can consult the patient's medical history or check the vital signs in advance to make a judgment and make a diagnosis. However, in the case of chronic bleeding, a small amount of bleeding, or bleeding under tumor conditions, it is necessary to detect occult blood (also known as occult blood) in the patient's gastric juice, vomit, or feces. Occult blood is "potential hemorrhage", that is, the presence of red blood cells cannot be observed under the naked eye or under a microscope, that is, hemorrhage that cannot be confirmed under the naked eye or under a microscope. Occult blood examination is of great value in diagnosing a var...

Claims

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Application Information

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IPC IPC(8): G01N33/72G01N21/78C08F220/48C08F226/06C08J7/00C08J5/18
CPCG01N33/721G01N21/78C08F220/48C08F226/06C08J7/00C08J5/18C08J2333/20C08J2339/04G01N2021/7759C08J7/065C08J2339/06
Inventor 张书鹏段晓东
Owner ANKON MEDICAL TECH SHANGHAI CO LTD
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