Serum preparation method and serum preparation apparatus

A preparation method and serum technology, applied in blood transfusion equipment, packaging, packaged food, etc.

Inactive Publication Date: 2010-01-27
JMS CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, such as collected donated blood, due to the anticoagulation treatment, it is impossible to remove the coagulation factors in the blood by attaching them to glass beads, so the method of Patent Document 2 is not applicable to this kind of blood

Method used

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  • Serum preparation method and serum preparation apparatus
  • Serum preparation method and serum preparation apparatus
  • Serum preparation method and serum preparation apparatus

Examples

Experimental program
Comparison scheme
Effect test

no. 1 Embodiment

[0061] 【Serum preparation method】

[0062] The present invention is a method for preparing serum using blood containing at least platelets, including a platelet treatment step of destroying the cell membranes of platelets in the blood.

[0063] The amount of cell proliferation factors that platelets can release automatically correlates with the freshness of the platelets. "High activity, fresh platelets" and "low activity, aging platelets" are mixed in the blood, and the amount of cell proliferation factors automatically released by "fresh platelets" is also large. The longer the time elapsed after blood collection, the more the content of "aging platelets", and the serum produced by using old blood has very little content of cell proliferation factors. However, in this embodiment, since the "platelet treatment process" that destroys the platelet cell membrane is provided, it is possible to obtain almost all the cell proliferation factors estimated based on the platelet conte...

no. 2 Embodiment

[0079] The difference between this embodiment and the first embodiment is that no anticoagulant is added to the blood when the serum is prepared.

[0080] In this example, the blood after the platelet processing step is in a so-called hemolyzed state because not only the platelets but also the cell membranes of erythrocytes and leukocytes are destroyed. In order to remove hemoglobin in red blood cells and DNA and RNA in white blood cells, it is necessary to remove components produced from red blood cells and white blood cells between the platelet treatment step and the precipitation step. Specifically, the removal treatment is removal using an adsorption column, a filter, and the like. Adsorption columns are preferred for removal of hemoglobin, and filters are preferred for removal of DNA and RNA.

no. 3 Embodiment

[0082] 【Serum preparation device】

[0083] Next, the serum preparation device for preparing serum in the above-mentioned present invention will be described.

[0084] The serum preparation device in the present invention prepares serum by using blood containing at least platelets, and includes a blood storage part for storing blood and a component storage part, and the component storage part is connected with the blood storage part in a sterile airtight manner for storing Serum separated from blood stored in a blood storage unit. Hereinafter, the serum preparation device in this embodiment will be described in detail.

[0085] figure 1 It is a schematic diagram of the serum preparation device in this embodiment. This serum preparation device 1 is mainly composed of a blood storage unit 10 and a component storage unit 20 . The blood storage unit 10 is composed of a whole blood storage unit 11 for storing whole blood of a donor, and a plasma storage unit 12 for storing plate...

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Abstract

The invention provides a serum preparation method and a serum preparation apparatus with which a large amount of serum capable of exhibiting a high culture efficiency regardless of freshness of blood to be used can be obtained at once. In a serum preparation method in which serum is prepared from blood containing at least platelets, a platelet treatment step in which a treatment of disrupting thecell membrane of the platelets in the blood is performed is provided. It is preferred that after the platelet treatment step, a deposition step in which a heat-labile protein in the blood is deposited and a removal step in which the heat-labile protein deposited by this deposition step is removed are provided.

Description

technical field [0001] The present invention relates to a method for preparing serum from collected blood and a device for preparing serum. Background technique [0002] At present, in the field of regenerative medicine, human stem cells are proliferated and differentiated in vitro, and then transplanted back into the body of the stem cell provider to promote the regeneration of human tissues. [0003] It is known that in the in vitro culture of stem cells, if serum is added to the culture medium, the culture efficiency can be improved. [0004] Patent Document 1 discloses a method of preparing blood serum using a blood collection tube filled with glass frit that promotes blood coagulation. Patent Document 2 discloses a method of separating collected blood by contacting blood coagulation-promoting glass beads to stimulate the activity of platelets, releasing cell growth factors, and then separating serum. . [0005] [Patent Document 1] JP-A-2000-000228 [0006] [Patent D...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K35/14A61J1/05G01N33/48A61K35/16A61M1/02A61M1/36
CPCA61K35/16A61M2202/0423A61M1/0209A61M1/029A61M1/0281A61M1/0218A61J1/05
Inventor 铃木康二田中圣真平井聪高畠真理
Owner JMS CO LTD
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