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Method for preparing L-lactic acid and special bacteria agent thereof

A technology of lactic acid and lactobacillus, applied in the field of preparation of L-lactic acid, which can solve the problems of low conversion rate of acidification, large energy consumption, and low product quality

Inactive Publication Date: 2009-12-30
INST OF MICROBIOLOGY - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Adopt rhizopus fermentation to have following shortcoming: (1) need higher air system and stirring power system, energy consumption such as electricity, steam is bigger, and production cost is higher; (2) because rhizopus fermentation is the heteromorphic fermentation of lactic acid, Therefore, in the process of fermenting lactic acid, the rhizopus carries out other pathways of metabolism at the same time, and a large amount of miscellaneous acids are accompanied, so that the conversion rate of acidification is low, and the product quality is low

Method used

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  • Method for preparing L-lactic acid and special bacteria agent thereof
  • Method for preparing L-lactic acid and special bacteria agent thereof
  • Method for preparing L-lactic acid and special bacteria agent thereof

Examples

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Effect test

Embodiment 1

[0020] Example 1. Isolation, purification and identification of Lactobacillus rhamnosus SMB-05, Lactobacillus salivarius SMB-06 and Lactobacillus paracasei SMB-07

[0021] 1. Isolation and purification of strains

[0022] Collect the oral saliva of different types of people and mix them thoroughly in sterile normal saline, then add the above-mentioned diluted oral saliva to the MRS liquid medium, anaerobic enrichment culture at 37°C for 48 hours, and obtain enrichment with pH3 On the MRS solid medium, cultivate anaerobically at 37°C for 24 hours, pick single colonies with larger transparent circles and inoculate them in the MRS liquid medium for anaerobic culture for 24 hours, observe whether they produce gas, and screen out the strains that do not produce gas , that is, homofermentative lactic acid bacteria; the homofermentative lactic acid bacteria obtained from the above screening were inoculated on the re-screening medium respectively, anaerobically cultured at 37°C for 48 ...

Embodiment 2

[0038] Embodiment 2, utilizing Lactobacillus rhamnosus (Lactobacillus rhamnosus) SMB-05 CGMCC № 3122, Lactobacillus salivarius (Lactobacillus salivarius) SMB-06 CGMCC № 3123 and Lactobacillus paracasei (Lactobacillus paracasei) SMB-07 CGMCC № 3124 fermentation Production of L-lactic acid

[0039] The composition of medium used in this embodiment is as follows:

[0040] MRS liquid medium: glucose 20g / L, peptone 10g / L, yeast powder 5g / L, beef extract 10g / L, K 2 HPO 4 2g / L, diammonium citrate 2g / L, sodium acetate 2g / L, Tween 80 1mL / L, MgSO 4 ·7H 2 O 0.58g / L, MnSO 4 4H 2 O 0.25g / L. During the fermentation process, the pH range is controlled at 5.5-6.2. Sterilization is carried out at 115°C for 20 minutes.

[0041] Fermentation medium: glucose 190g / L, peptone 10g / L, beef extract 10g / L, yeast powder 5g / L, K 2 HPO 4 3g / L, KH 2 PO 4 3g / L, sodium acetate 2g / L, MgSO 4 ·7H 2 O 0.58g / L, MnSO 4 4H 2 O 0.25g / L, Tween-80 1ml / L, CaCO 3 30g / L. pH5.0~5.5. Sterilize at 115℃ ...

Embodiment 3

[0053] Embodiment 3, utilizing Lactobacillus rhamnosus (Lactobacillus rhamnosus) SMB-05 CGMCC № 3122, Lactobacillus salivarius (Lactobacillus salivarius) SMB-06 CGMCC № 3123 and Lactobacillus paracasei (Lactobacillus paracasei) SMB-07 CGMCC № 3124 fermentation Production of L-lactic acid

[0054] The composition of the culture medium used in this example is the same as in Example 2.

[0055] Lactobacillus rhamnosus (Lactobacillus rhamnosus) SMB-05CGMCC No. 3122, Lactobacillus salivarius (Lactobacillus salivarius) SMB-06 CGMCC No. 3123 and Lactobacillus paracasei (Lactobacillus paracasei) SMB-07 CGMCC No. 3124 were inoculated into MRS liquid medium respectively, and cultured anaerobically at 37°C to the logarithmic growth phase, so that the concentration of the bacteria liquid reached 10 9 ~10 11 cfu / mL. The above-mentioned three kinds of lactobacilli are mixed according to a certain ratio, so that Lactobacillus rhamnosus (Lactobacillusrhamnosus) SMB-05 CGMCC № 3122, Lactoba...

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Abstract

The invention discloses a method for preparing L-lactic acid and a special bacteria agent thereof. The method comprises the steps: fermenting and culturing lactobacillus rhamnosus SMB-05 CGMCC N03122, lactobacillus salivarius SMB-06 CGMCC N03123, and lactobacillus paracasei SMB-07 CGMC N03124, and obtaining the L-lactic acid. The experiment result shows that the fermentation is carried out for 37 hours, the quantity of the total lactic acid in the fermentation liquor is 216 to 241g / L, the optical purity of the L-lactic acid is 98 percent to 99 percent and the fermentation production rate is 2.2 to 6.5g / L-h. By the mixed fermentation technology, the invention improves the production quantity and the production rate of the L-lactic acid, greatly reduces the cost of the L-lactic acid and has important industrial application value.

Description

technical field [0001] The invention relates to a method for preparing L-lactic acid and a special bacterial agent thereof. Background technique [0002] With the continuous reduction of petroleum resources and the rising price of non-renewable resources, the development and utilization of more environmentally friendly bio-based products has become a strategic demand for transforming the mode of economic growth, ensuring a virtuous cycle of the ecological chain, and achieving sustainable economic and social development. Lactic acid is an important and versatile bio-based platform compound with two isomers, L-(+) and D-(-) lactic acid. As an important biochemical material, L-lactic acid is widely used in food, medicine, modern agriculture, daily chemical industry and organic chemical industry, covering a large number of products. One of the most important and widespread industrial applications is for the production of degradable polymers - polylactic acid (PLA). Polylactic ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12P7/56C12R1/225
Inventor 徐洪涛朱林江张延平李寅
Owner INST OF MICROBIOLOGY - CHINESE ACAD OF SCI
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