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Preparation method of HA/collagen/PVP semi-interpenetrating polymer network scaffold

A polymer and semi-interpenetrating technology, applied in medical science, prosthesis, etc., can solve the problems of preparation without engineering scaffolds, and achieve the effects of good physiological safety, excellent dispersion stability, and good biocompatibility

Inactive Publication Date: 2009-12-30
SOUTH CHINA UNIV OF TECH +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, this technique has not been used in the preparation of collagen / PVP semi-IPP network bone tissue engineering scaffolds

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0046] A preparation method of HA (nano hydroxyapatite) / collagen / PVP (polyvinylpyrrolidone) semi-interpenetrating polymer network scaffold comprises the following steps and process conditions:

[0047] Step 1: Preparation of HA sol

[0048] (1) Using PVP K15 as template agent, dispersant and modifier at the same time;

[0049] (2) use deionized water to prepare the solution containing calcium nitrate 0.01mol / L, diammonium hydrogen phosphate 0.006mol / L, then 0.1g PVP K15 is added into the above calcium nitrate solution and fully dissolved;

[0050] (3) using a high-speed disperser of 100 rpm, under the condition that the pH value of the ammonia water control system is 10.6, the materials of the above (1) and (2) are fully mixed to form HA sol, so that calcium in the HA sol system is The phosphorus molar ratio is 1.6:1, and the PVP content is 0.01% by mass percentage;

[0051] Step 2: Aging of HA sol

[0052] The above-mentioned HA sol is naturally placed and aged for 24 hour...

Embodiment 2

[0063] A preparation method of a HA / collagen / PVP semi-interpenetrating polymer network scaffold comprises the following steps and process conditions thereof:

[0064] Step 1: Preparation of HA sol

[0065] (1) Using PVP K30 as template agent, dispersant and modifier at the same time;

[0066] (2) use the PVP K30 aqueous solution of 0.5% by mass to prepare a solution containing calcium nitrate 0.1 mol / L and disodium hydrogen phosphate 0.06 mol / L;

[0067] (3) using a high-speed disperser of 1000 rpm, under the condition that the pH value of the sodium hydroxide control system is 11, the materials of the above (1) and (2) are fully mixed to form a nano-hydroxyapatite sol, The molar ratio of calcium and phosphorus in the nano-hydroxyapatite sol system is 1.65:1, and the PVP content is 0.5% by mass percentage;

[0068] Step 2: Aging of HA sol

[0069] Move the above HA sol into a heating, evaporation and reflux device, and evaporate and reflux at 50°C for 36 hours for aging; th...

Embodiment 3

[0080] A preparation method of a HA / collagen / PVP semi-interpenetrating polymer network scaffold comprises the following steps and process conditions thereof:

[0081] Step 1: Preparation of HA sol

[0082] (1) Using PVP K60 as template agent, dispersant and modifier at the same time;

[0083] (2) use the PVP K60 aqueous solution of 5% by mass to prepare a solution containing calcium nitrate 0.5mol / L and dipotassium hydrogen phosphate 0.3mol / L;

[0084] (3) using a high-speed disperser of 2000 rpm, under the condition that the pH value of the potassium hydroxide control system is 11.5, the materials of the above (1) and (2) are fully mixed to form an HA sol, so that the HA sol system is The molar ratio of calcium to phosphorus reaches 1.67:1, and the PVP content is 5% by mass percentage;

[0085] Step 2: Aging of HA sol

[0086] Move the above HA sol into a heating, evaporation and reflux device, evaporate and reflux at 70°C for 48 hours, and then fully wash the sol with dei...

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Abstract

The invention discloses a preparation method of an HA / collagen / PVP semi-interpenetrating polymer network scaffold. The method comprises the following steps: thoroughly mixing in an alkali solution control system to form a sol system with the PVP content of 0.01-50% (by weight); aging and cleaning to obtain HA sol, and then mixing with blended solution with a mass ratio of PVP to collagen solute being 0.1-2.5:1; adding the obtained mixture containing the blended solution with the mass ratio of the solute to HA sol being 0.1-9:1 to a high-speed dispersion machine for mixing and crosslinking; and injecting the mixture into a mould, followed by post treatment to obtain the scaffold. HA prepared by the method is evenly distributed on a collagen / PVP semi-interpenetrating polymer network matrix and effectively linked with the matrix, and is in a directional arrangement, thus realizing effective combination of nano hydroxyapatite with the matrix in structure and function, and effectively solving problems existing in the collagen material. The preparation method has simple process and convenient operation and is easy to popularize and apply.

Description

technical field [0001] The invention relates to the technical field of artificial bone repair materials, in particular to a preparation method of a HA (nano hydroxyapatite) / collagen / PVP (polyvinylpyrrolidone) semi-interpenetrating polymer network scaffold. Background technique [0002] With the increasing demand for bone defect repair in clinical medicine at home and abroad, the traditional artificial bone repair material is quite different from the highly precise mineralized structure of natural bone, so the problem of bone defect repair has not been effectively solved. Therefore, as an artificial bone repair material, bionics in terms of composition and structure is the main direction of current and future development. Human bone tissue is a highly precise natural mineralized biological structural material assembled with nano-hydroxyapatite and collagen. Therefore, in order to simulate the composition and structure of natural bone, the composite of nano-hydroxyapatite and...

Claims

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Application Information

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IPC IPC(8): A61L27/40A61L27/56A61L27/24A61L27/16A61L27/12
Inventor 王迎军宁成云尹兆益谭帼馨
Owner SOUTH CHINA UNIV OF TECH
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