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Method for cleanly producing lactic acid by fermentation method

A clean production and fermentation technology, applied in fermentation, separation/purification of carboxylic acid compounds, organic chemistry, etc., can solve the problems of calcium sulfate waste residue, salty waste liquid, etc., to solve pollution problems, speed up, The effect of increasing the growth rate

Active Publication Date: 2009-12-23
INST OF PROCESS ENG CHINESE ACAD OF SCI
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  • Abstract
  • Description
  • Claims
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Problems solved by technology

[0023] The purpose of this invention is to provide a kind of method that utilizes anion exchange and bipolar membrane electrodialysis to carry out fermentation method clean production lactic acid, overcome the defect that produces calcium sulfate waste residue when producing lactic acid in existing production technology, overcome the ion exchange method of report, direct electrodialysis The dialysis extraction method will produce the problem of saline waste liquid

Method used

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  • Method for cleanly producing lactic acid by fermentation method
  • Method for cleanly producing lactic acid by fermentation method
  • Method for cleanly producing lactic acid by fermentation method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0101] Step 1) preparing lactic acid fermentation broth, wherein the pH value of the fermentation broth is adjusted with ammonia water.

[0102] The microbial strain used was Lactobacillus paracasei (Lactobacillus paracasei, NERCB0401 (Industrial Microbiology, 2007, Vol. 37, Issue 4, 1-5)), and fed-batch fermentation was carried out with the above strain. There are two types of media used:

[0103] (1) Seed medium: glucose 40g / L, peptone 10g / L, beef extract 10g / L, yeast powder 5g / L, diammonium hydrogen citrate 2g / L, sodium acetate 5g / L, dipotassium hydrogen phosphate 2g / L L, Tween 801mL / L, magnesium sulfate 0.6g / L, manganese sulfate 0.25g / L.

[0104] (2) Fermentation medium: glucose 60g / L, peptone 10g / L, beef extract 6g / L, yeast powder 10g / L, sodium chloride 0.03g / L, ferrous sulfate 0.01g / L, sodium acetate 4g / L , diamine citrate 2g / L, potassium dihydrogen phosphate 2g / L, Tween-801mL / L, magnesium sulfate 0.2g / L, manganese sulfate 0.2g / L.

[0105] Prepare two kinds of medium ...

Embodiment 2

[0116] Step 1) is the same as step 1) of Example 1 to prepare a lactic acid fermentation broth to obtain about 3.4 liters of a lactic acid fermentation broth with a lactic acid concentration of 142 g / L.

[0117] Step 2) The ion-exchange column is the same as step 2) of Example 1, wherein the D290 anion-exchange resin packed is converted into sulfate radical type in advance. 2.3 liters of the lactic acid fermented liquid obtained in step 1) are passed through the ion exchange column, so that the lactic acid radical in the lactic acid fermented liquid is replaced by SO 4 2- exchange adsorption. The upper column flow rate is 1.0 column volume / hour, and about 2.2L of ionized permeate containing 0.75mol / L ammonium sulfate is collected at the bottom of the column. The measured pH is 5.9, COD is 25300mg / L, and the calcium and magnesium content is about 200mg / L. L.

[0118] Step 3) Elution of lactic acid

[0119] Use about 0.9mol / L of sulfuric acid to elute step 2) the ion exchang...

Embodiment 3

[0124] Step 1) is the same as step 1) of Example 1 to prepare a lactic acid fermentation broth to obtain about 3.5 liters of a lactic acid fermentation broth with a lactic acid concentration of 142 g / L.

[0125] Step 2) The ion-exchange column is the same as step 2) of Example 1, wherein the D290 anion-exchange resin packed is converted into the nitrate radical type in advance. Pass 2.4 liters of the lactic acid fermentation liquid obtained in step 1) through the ion exchange column, so that the lactic acid radicals in the lactic acid fermentation liquid are covered by NO 3 - exchange adsorption. The upper column flow rate is 1.0 column volume / hour, and about 2.2L of ionized permeate containing 1.5mol / L ammonium nitrate is collected at the bottom of the column. The pH is 5.8, the COD is 24500mg / L, and the calcium and magnesium content is about 200mg / L. L.

[0126] Step 3) Elution of lactic acid

[0127] Use about 2.0mol / L of nitric acid to elute step 2) The ion exchange co...

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Abstract

The invention relates to a method for cleanly producing lactic acid by a fermentation method. The method comprises the following steps that: during the fermentation of the lactic acid, solution of NH3 and NaOH or solution of KOH is used for adjusting the pH value of fermentation solution, the obtained fermentation solution of the lactic acid passes through a C1 typed, sulfate radical typed or nitrate radical typed anion-exchange column, lactate radical in the fermentation solution of the lactic acid is exchanged and adsorbed by C1-, SO42- or NO3-, and simultaneously ionic exchange permeating solution of hydrochloride, sulfate or nitrate containing NH4+, Na+ or K+ and permeating the ionic exchange column is obtained; the lactic acid adsorbed on the ionic exchange column and exchanged and adsorbed by the lactate radical is eluted by hydrochloric acid, sulfuric acid or nitric acid, an eluent containing the lactic acid is obtained, and the ionic exchange column is regenerated simultaneously during eluting; the ionic exchange permeating solution is filled in a bipolar membrane electrodialyzer to obtain regenerated hydrochloric acid, sulfuric acid or nitric acid; the regenerated hydrochloric acid, the sulfuric acid or the nitric acid is used for eluting the lactic acid adsorbed on the ionic exchange column, and the regenerated solution of the NH3 and the NaOH or the solution of the KOH is used for adjusting the pH value of the fermentation solution when the lactic acid is fermented.

Description

technical field [0001] The invention belongs to the field of fermentation industry or organic acid industry, and in particular relates to a method for cleanly producing lactic acid by fermentation. Background technique [0002] Lactic acid and its salts and esters are widely used. Traditionally, lactic acid and its salts and esters are mainly used as additives in the food industry and cleaning agents and solvents in the chemical industry; the application of lactic acid and its salts and esters in medicine and agriculture has also made great progress. Since the human body cannot decompose D-lactic acid, excessive intake of D-lactic acid will lead to the accumulation of D-lactic acid and cause metabolic disorders. Developed countries in Europe and the United States have banned the use of D-lactic acid and DL-lactic acid in food. This becomes an important reason for the increased demand for L-lactic acid. In recent years, with the rise of poly-L-lactic acid, the production o...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07C59/08C07C51/47C12P7/56
Inventor 丛威郭广威王倩杨鹏波吴霞张勇
Owner INST OF PROCESS ENG CHINESE ACAD OF SCI
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