Method for reducing the content of poplar lignin with RNA interference

Abstract

The invention discloses a method for reducing the content of poplar lignin with RNA interference in the field of biological technology; CCR gene segment is cloned from poplar and is used as an RNA interference segment, a constitutive promoter CaMV35S is used for promoting and expressing to construct an expression vector containing CCR gene front-back segments, agrobactrium tumefaciens is used for mediating, the front-back structure of the CCR gene is converted to the poplar, by the reactive plant PCR detection and the detection of lignin and holocellulose of transgenic poplar, the lignin content of the transgenic plant is reduced by 9.7 percent in an average way compared with the lignin content of the non-transgenic plant, the holocellulose content of the transgenic plant is improved 3.17 in an average way compared with the holocellulose content of the non-transgenic plant,, thereby providing a method for reducing the content of poplar lignin, so as to establish the basis for providing papermaking raw material in a large scale by utilizing the transgenic poplars.

Description

technical field [0001] The invention relates to a method for reducing the Klason lignin content of poplar in the field of biotechnology, in particular to a method for reducing the Klason lignin content of poplar by RNA interference. Background technique [0002] Paper is a necessity of people's life. At present, the world's pulp and paper industry mainly uses wood as the raw material for paper fiber. The basic principle of the pulp and paper process is to separate the fibers in the plant raw materials by chemical, mechanical or combined chemical and mechanical methods, and then mix them in pulp and water. The fibers are re-interwoven into a uniform and dense paper in the suspension, and the separation of the fibers is essentially a process of plasticizing or dissolving the lignin contained in the fibers. With the rapid development of the economy and the improvement of people's living standards, not only the types and quantities of paper are more and more required, but also ...

AI Technical Summary

Problems solved by technology

However, the application of antisense technology has certain limitations. Its inhibition of endogenous gene expression is weak, and it often produces transitional phenotypes, which will hinder the accurate judgment of the function of the target gene.

The genetic stability of its inhibitory effect is poor, and it cannot stably and reliably reduce the expression of the target gene

[0006] 2. Gene knockout technology and gene conversion technology mediated by DNA / RNA chimeric molecules, but this method can only study one gene at a time, and cannot effectively knock out multi-gene families

Some genes that play a key role in the development of animals and plants, if they are heritably modified by gene knockout or mutation at the DNA level, will be prematurely silenced and produce lethal phenotypes, so they cannot be studied in depth

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