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Composition preparation of 24-methylene cycloartenol feruloyl esterase and purification process thereof

A technology of pineapple ferulate and methylene cyclowood, which is applied in the field of purifying cycloartenol ferulate, can solve the problem of high cost and inability to obtain high-purity oryzanol 24-methylene cyclowood Solve problems such as alcohol ferulic acid ester components, achieve the effects of low cost, high yield and broad application prospects

Active Publication Date: 2009-12-02
BEIJING CENTURY BIOCOM PHARMA TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0012] The present invention overcomes the problems existing in the current technical field that high-purity 24-methylene cycloartenyl ferulic acid ester components in oryzanol cannot be obtained and its actual medicinal use, etc., and overcomes the simple high-efficiency liquid phase preparation by using processes such as recrystallization High cost of high-purity 24-methylenecycloartenol ferulate

Method used

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  • Composition preparation of 24-methylene cycloartenol feruloyl esterase and purification process thereof
  • Composition preparation of 24-methylene cycloartenol feruloyl esterase and purification process thereof
  • Composition preparation of 24-methylene cycloartenol feruloyl esterase and purification process thereof

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Take 1kg of oryzanol, heat and dissolve it with about 8kg of acetone, let stand at room temperature to precipitate crystals, filter, and dry. The above process was repeated 10 times for the dried crystals. The content of 24-methylene cycloartenyl ferulic acid ester reaches more than 99%.

Embodiment 2

[0035] 1. Chromatographic conditions and system suitability test: octadecylsilane bonded silica gel is used as filler; methanol-acetonitrile (40:60) is used as mobile phase; flow rate is 1.5ml / min; detection wavelength is 327nm; sensitivity is 0.005 AUFS. The number of theoretical plates should not be less than 2000 according to the calculation.

[0036] 2. Preparation of the test solution: Accurately weigh 10 mg of the commercially available oryzanol raw material and 10 mg of the present invention, put them in a 10 ml measuring bottle, add water to dissolve and constant volume, and shake well to obtain the test solution.

[0037] 3. Determination: Accurately draw 10 μl of the test solution, inject it into a liquid chromatograph, record the chromatogram, and check the percentage content of each component by the area normalization method.

[0038] 4. Measurement results

[0039] Table 2 Measurement results

[0040]

[0041] Illustrate that technique of the present inventi...

Embodiment 3

[0043] prescription

[0044] 1000 bottles of freeze-dried powder of the present invention for injection (containing 18mg / bottle of the present invention):

[0045] Purified product of the present invention 18g

[0046] Macrogol 400 250g

[0047] Tween 80 250g

[0048] 10% Mannitol 6000g

[0049] Add water for injection to 5000ml

[0050]

[0051] A total of 1000 tubes were freeze-dried

[0052] In the prescription, the raw material of the present invention is used as the main agent, Tween 80 is used as a solubilizing agent, polyethylene glycol 400 is used as a cosolvent, mannitol is used as a proppant, and water for injection is used as a freeze-drying solvent.

[0053] Preparation process

[0054] Take by weighing the purified product of the present invention, Tween-80, PEG-400, mannitol and 80% of the prescription amount of water for injection, stir to make it dissolve until the solution is clear, stir at ro...

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Abstract

The invention discloses a composition preparation of 24-methylene cycloartenol feruloyl esterase, and a purification process thereof. The purity of 24-methylene cycloartenol feruloyl esterase purified by the purification process can reach more than 80%. The composition disclosed by the invention contains 24-methylene cycloartenol feruloyl esterase and surface active agent, wherein, the surface active agent is selected from Tween 80, polyethylene glycol 400 or mixture thereof. Meanwhile, the invention also discloses an injection of the composition, in particular to freeze-dry powder injection for injection.

Description

technical field [0001] The invention discloses a process for purifying 24-methylenecycloartenol ferulic acid ester and its preparation. Further, the invention discloses a process for purifying cycloartenol ferulic acid from oryzanol raw materials by using recrystallization technology Ester process. Background technique [0002] With the gradual acceleration of the pace of life and work in modern society, the sub-health status of modern people is not optimistic. According to statistics, patients with autonomic disorders, menopausal syndrome, primary dysmenorrhea, premenstrual tension, etc. due to autonomic dysfunction, lead to the imbalance of sympathetic and parasympathetic nerves, and the incidence of non-organic diseases is increasing year by year. trend. [0003] Autonomic disorders are usually caused by mental trauma, long-term tension and fatigue, which lead to weakening of the function of the cerebral cortex, excessive excitement and rapid fatigue. Menopausal syndro...

Claims

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Application Information

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IPC IPC(8): A61K9/00A61K31/56A61P25/00C07J63/00
Inventor 郝守祝何乐
Owner BEIJING CENTURY BIOCOM PHARMA TECH
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