Cosmetic composition for protecting skin against uv light and wrinkle improvement containing the extract of magnolia sieboldii flower extracts
A cosmetic composition, the technology of Tiannv Mulan, applied in the direction of skin care preparations, cosmetics, cosmetic preparations, etc., can solve the problems of skin aging, skin cancer increase, etc., and achieve the effect of reducing biosynthesis, reducing skin wrinkles, and excellent effects
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preparation example 1
[0049] Preparation Example 1: Preparation of Flower Extract of Magnolias
[0050] 100 g of dried flowers of Magnolias (purchased from Kyung Dong Market, Korea) were added to 1.0 L of 70% ethanol (ethanol: water = 7: 3), and extracted at room temperature for 3 days. The extracted solution was filtered, and the filtrate was concentrated under vacuum to obtain a dried extract (extraction rate: 7.8 g / 100 g). The dried extract was dissolved in 30% 1,3-butanediol solution to a concentration of 1% (w / v), and then filtered. The obtained extracts were then used in the experiments of the present invention.
preparation example 2
[0051] Preparation Example 2: Preparation of the Whole Plant Extract of Magnolias
[0052] Add 100 g of dried whole plant of Magnolia magnolia (purchased from Kyung Dong Market, Korea) into 1.0 L of 70% ethanol (ethanol:water=7:3), and extract at room temperature for 3 days. The extracted solution was filtered, and the filtrate was concentrated under vacuum to obtain a dry extract (extraction rate: 7.0 g / 100 g). The dried extract was dissolved in 30% 1,3-butanediol solution to a concentration of 1% (w / v), and then filtered. The obtained extracts were then used in the experiments of the present invention.
experiment example 1
[0053] Experimental example 1: Inhibition of keratinocyte cell death caused by ultraviolet rays
[0054] When keratinocytes are irradiated with ultraviolet rays, active oxygen is generated in the cells. To artificially induce this phenomenon, normal human keratinocytes, namely Hacat cells (Korea Cell Line Bank), were seeded into each well of a 48-well microplate at a density of 1×10 4 cells / well, and cultured at 37° C. for 24 hours in DMEM medium (Sigma, USA). The cultured cells were irradiated with ultraviolet rays (UVB), and then the magnolia extract of Preparation Example 1 was added to the cell culture in each well at concentrations of 0.1, 0.5, 1.0 and 2.0% (v / v). After 24 hours, cell viability was analyzed by MTT assay.
[0055] Table 1
[0056]
[0057] It can be seen from the above Table 1 that the Magnolia extract of the present invention inhibits keratinocyte death caused by ultraviolet irradiation in a concentration-dependent manner, and exhibits an excellent ...
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