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Earthworm protein polypeptide preparation

A ground dragon protein and preparation technology, which is applied in the fields of peptide/protein components, medical preparations containing active ingredients, cardiovascular system diseases, etc., can solve the problems of active influence, complicated steps, unstable component properties, etc. High concentration, increase blood pressure, and enhance the effect of activity

Inactive Publication Date: 2012-12-19
NANJING UNIVERSITY OF TRADITIONAL CHINESE MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] In the prior art, to separate the protein components in Earthworm, the homogenate solution is usually used for salting out, ion exchange chromatography, gel filtration chromatography and other biochemical separation methods. Although it is feasible to a certain extent, the steps are complicated and the experimental conditions require High, not suitable for large-scale production, and very different from traditional Chinese medicine syndrome differentiation
[0004] Protein and polypeptide components are the main active components of Earthworm. Due to the unstable properties of protein and polypeptide components, they have high environmental requirements, and acid, alkali, ionic strength, temperature or light will affect their activity. Therefore, the traditional method of using water Decocted into medicine, affecting its efficacy

Method used

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  • Earthworm protein polypeptide preparation

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] The preparation of embodiment 1 earthworm protein polypeptide preparation

[0037]① Take 100g of Earthworm, add 500ml of double distilled water, homogenate for 30 minutes, centrifuge at 15000r / min for 30 minutes at 4°C, take the supernatant of Earthworm after centrifugation, and set aside;

[0038] ②Take 60ml of the earthworm supernatant (containing about 74.4mg of protein) obtained in step ①, add 0.7mg of trypsin at a concentration of 250U / mg, 0.7mg of chymotrypsin at a concentration of 1000U / mg and elastin at a concentration of 30U / mg Protease 0.7mg, make the weight percentage of the mixed enzyme reach 3%, then add sodium hydroxide to adjust the pH value to 8.0, and enzymatically hydrolyze in a water bath at 50°C for 5 hours;

[0039] ③ Take the enzymatic hydrolyzate obtained in step ② and a pharmaceutically acceptable carrier to prepare the terrapenin polypeptide preparation.

[0040] The enzymatic hydrolyzate of the ground dragon protein polypeptide obtained in the...

Embodiment 2

[0041] The preparation of embodiment 2 earthworm protein polypeptide preparations

[0042] ① Take 100g of Earthworm, add 300ml of double distilled water, homogenate for 10 minutes, centrifuge at 11000r / min for 20 minutes at 4°C, take the supernatant of Earthworm after centrifugation, and set aside;

[0043] ②Take 60ml of the earthworm supernatant obtained in step ① (containing about 74.4mg of protein), add 1.5mg of trypsin at a concentration of 200U / mg, 1mg of chymotrypsin at a concentration of 800U / mg and elastase at a concentration of 20U / mg 1.2mg, make the weight percentage of the mixed enzyme reach 5%, then add sodium hydroxide to adjust the pH value to 8.0, and enzymatically hydrolyze in a water bath at 50°C for 2 hours;

[0044] ③ Take the enzymatic hydrolyzate obtained in step ② and a pharmaceutically acceptable carrier to prepare the terrapenin polypeptide preparation.

[0045] The enzymatic hydrolyzate of the ground dragon protein polypeptide obtained in the present ...

Embodiment 3

[0046] The preparation of embodiment 3 earthworm protein polypeptide preparations

[0047] ① Take 100g of Earthworm and add 300ml of distilled water, homogenate for 20 minutes, centrifuge at 12000r / min for 30 minutes at 4°C, take the supernatant of Earthworm after centrifugation, and set aside;

[0048] ②Take 100ml of the earthworm supernatant obtained in step ① (containing about 92.5 grams of protein), add 0.9mg of trypsin at a concentration of 250U / mg, 0.9mg of chymotrypsin at a concentration of 1000U / mg and elastase at a concentration of 30U / mg Protease 0.9mg, make the weight percentage of the mixed enzyme reach 3%, then add sodium hydroxide to adjust the pH value to 8.0, and enzymatically hydrolyze in a 50°C water bath for 5 hours;

[0049] ③Take 100ml of the enzymolysis solution obtained in step ②, under the conditions of the temperature of the enzymolysis solution at 50°C, the operating pressure at 0.25MPa, and the surface speed at 200r / min, use pore diameters of 50,000,...

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Abstract

The invention discloses an earthworm protein polypeptide preparation, its preparation method and application. The protein polypeptide preparation is prepared by hydrolyzing the earthworm homogenate coarse extract or hydrolyzing the earthworm homogenate coarse extract to obtain the hydrolyzed protein polypeptide, separating by a hyperfiltration membrane with different hole diameter to obtain the protein polypeptide ingredients with different hole diameter. Test proves that the earthworm protein polypeptide preparation has good angiotensin converting enzyme after enzyme hydrolysis and can effectively prevent the high blood pressure. The invention provides a preparation method for the earthworm protein polypeptide preparation with strong operability, high efficiency and realizes an industrialization big production.

Description

technical field [0001] The present invention relates to a protein polypeptide preparation, in particular to a ground dragon protein polypeptide preparation, its preparation method and its application in the prevention and treatment of hypertension Background technique [0002] Earthworm, cold in nature and salty in taste, has the functions of clearing away heat and relieving wind, relieving asthma, dredging collaterals, and diuresis. It is not only anticoagulant, inhibits platelet aggregation, antiarrhythmia, but also has anticancer and analgesic effects. The crude extract obtained from earthworm homogenate has complex components, and its molecular weight is in the range of 12000 to 80000D. In addition to the above macromolecules, Earthworm also contains some small molecules, such as earthworm, earthworm antipyretic, nucleic acid derivatives, vitamins and inorganic salts, etc., and the current clinical use of earthworm is mainly the crude extract obtained from earthworm homo...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K38/02A61P9/12A61K35/64A61K35/62
Inventor 詹秀琴戴启刚郭立玮樊文玲
Owner NANJING UNIVERSITY OF TRADITIONAL CHINESE MEDICINE
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