Chip for screening viruses of cucumovirus and application thereof

Abstract

The invention discloses a chip for screening viruses of cucumovirus and application thereof. The chip is a DNA chip which fixes at least 16 DNA fragment arrays on a carrier surface, wherein the nucleotide sequences of the 16 DNA fragment arrays are sequences 1 to 16 in the sequence table, respectively. The method analyzes the nucleotide sequences of the cucumovirus by bioinformatics method, designs compatible probes for the cucumovirus, and by standard virus source verification, establishes a set of chip-based screening technical system of emerging viruses of the cucumovirus in the world, and effectively overcomes the defects of the existing monitoring methods, thus strengthening the ability of early warning the emerging viruses of the cucumovirus.

Description

technical field [0001] The invention relates to a chip for screening cucumber mosaic virus and its application. Background technique [0002] The invention relates to the technical fields of bioinformatics and biological detection and identification, in particular to a probe and an operating system for identifying known and unknown viruses of the genus Cucumovirus. It is mainly used in the quarantine of Cucumber mosaic virus in the entry-exit inspection and quarantine system, the diagnosis of plant virus diseases in plant protection stations, and the virus identification in the study of plant virus taxonomy. [0003] According to the eighth classification report of the International Committee on Taxonomy of Viruses (ICTV), there are three confirmed viruses in the genus Cucumovirus, namely, cucumber mosaic virus (CMV), peanut dwarf Peanut stunt virus (PSV), tomato infertility virus (Tomato aspermy virus, TAV). Cucumber mosaic virus, a representative species, has a wide host...

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Problems solved by technology

[0004] Due to the possibility of potential new quarantine viruses in this genus and the existence of multiple groups, specific antiserum and primers are prone to missed detection and wrong detection when detecting and identifying known viruses, and it is impossible to identify possible viruses in this genus. Identification of unknown viruses, which leads to an increased possibility of the spread of dangerous viruses, increasing the risk of economic loss and social impact

[0005] The current detection methods for CMV include biological methods, immunological methods, electron microscopy techniques, molecular biology techniques, etc. These methods are mainly for the specific detection of known viruses of CMV. Surveillance of new, emerging viruses is powerless

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