Immuomagnetic bead chromatographic test strip for rapidly detecting algae toxin and preparation method thereof

Abstract

The invention discloses an immuomagnetic bead chromatographic test strip for rapidly detecting algae toxin and a preparation method thereof, relating to the technical field of algae toxin detection. The test strip consists of a lining board as well as a sample pad, a magnetic scale combination pad, a coated film and an absorbing pad which are sequentially engaged on the lining board; the magnetic scale combination pad is glass fiber for absorbing algae toxin magnetic scale antibody; and the coated film is provided with an adelomorphic detection line T line which is printed by algae toxin-coupled carrier protein solution, and an adelomorphic control line C line which is printed by goat anti-rabbit IgG solution. The sample pad of the test strip is inserted into the solution of a tested sample, then taken out after 10-20 seconds, and then reacts for 15 minutes at room temperature; the test strip is arranged in a magnetic signal detector for detection, and the detector further outputs the biological responding signals which have been converted into magnetic field signals in the form of electrical signals; and after a specification curve is drawn, the algae toxin content in the tested sample is calculated according to the specification curve. The invention relates to the method based on nano-magnetic immunological technique for detecting algae toxin, and has the characteristics of high sensitivity, short reaction time, cheap instrument and equipment, convenience, easy usage and the like.

Description

technical field [0001] The invention discloses a nano-immune magnetic bead chromatography test strip for rapid detection of algal toxins and a preparation method thereof, relating to the technical field of algal toxin detection. Background technique [0002] With the development of the economy, sewage containing a large amount of nitrogen and phosphorus nutrients enters lakes and reservoirs, causing eutrophication of water bodies, causing abnormal proliferation of algae, releasing secondary metabolites algal toxins (Algae Toxins), threatening the safety of human drinking water. safety and the safety of other organisms in the water body. Among them, Microcystins (Microcystins, MCs) are widely distributed and more harmful. They are a family of cyclic heptapeptides with similar structures produced by certain species of freshwater cyanobacteria, mainly Microcystis aeruginosa. There are more than 60 isomers known. Its structure is as follows: [0003] [0004] It contains f...

AI Technical Summary

Problems solved by technology

Instrumental analysis methods mainly include high-performance liquid chromatography (HPLC), mass spectrometry, liquid chromatography-mass spectrometry, etc. Although these methods are sensitive, they require expensive instruments and equipment, professional operators, and relatively high requirements for inspection materials. Further sample pretreatment can be carried out, which can no longer meet the requirements of modern detection for fast, convenient and accurate

The biochemical method is mainly the protein phosphatase inhibition test method, which has the advantage of being fast and can detect a large number of samples in a few hours, but its biggest disadvantage is that specific protein phosphatases are not commercially available and have poor specificity

There is no report on the development of rapid diagnostic test strips for the detection of algal toxin antibodies using nano-immune magnetic beads

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