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Method for preparing poly(amidoamine)/carbon nanometer tube composite material for cancer cell targeting diagnosis

A technology of carbon nanotubes and polyamidoamines, which is applied in the field of preparation of polyamidoamine/carbon nanotube composites for cancer cell-targeted diagnosis, achieving the effects of easy operation, good biocompatibility, and mild reaction conditions

Inactive Publication Date: 2009-09-16
DONGHUA UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] A search of domestic and foreign literature and patents on the functionalization of carbon nanotubes shows that there is no relevant report on the use of dendrimers to functionalize carbon nanotubes for the targeted diagnosis and treatment of cancer cells.

Method used

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  • Method for preparing poly(amidoamine)/carbon nanometer tube composite material for cancer cell targeting diagnosis
  • Method for preparing poly(amidoamine)/carbon nanometer tube composite material for cancer cell targeting diagnosis
  • Method for preparing poly(amidoamine)/carbon nanometer tube composite material for cancer cell targeting diagnosis

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0061] (1) Configure a mixture of concentrated nitric acid and concentrated sulfuric acid with a volume ratio of 3:1 (ie HNO 3 / H 2 SO 4 For v / v=3:1), acidify 100-200mg of multi-walled carbon nanotubes, after 2 hours, filter, wash with water, and finally place the acidified multi-walled carbon nanotubes MWCNTs under vacuum conditions for drying and storage ;

[0062] (2) In 24mL of anhydrous DMSO solvent containing 60mg PAMAM, add 24mL of DMSO solution containing 4.4mg FI dropwise dropwise, stir with strong magnetic force at room temperature for 24 hours. Dialyze in PBS buffer solution (3 times, 4 liters) and ultrapure water (3 times, 4 liters) for 3 days, remove the excessive reaction reagent in the reaction mixture and the by-product produced in the reaction process, finally the product after purification Freeze-dried to get MG5.NH 2 -FI;

[0063] (3) Take 16.68 mg of acidified MWCNTs and disperse them in 12 mL of ultrapure water, then drop 2 mL of DMSO solution contain...

Embodiment 2

[0067](1) In 24mL of anhydrous DMSO solvent containing 60mg PAMAM, add 24mL of DMSO solution containing 4.4mg FI dropwise dropwise, stir with strong magnetic force at room temperature for 24 hours, then use MWCO10000 cellulose dialysis membrane to buffer the reaction product successively in PBS Solution (3 times, 4 liters) and ultrapure water (3 times, 4 liters) were dialyzed for 3 days to remove excess reaction reagents and by-products produced during the reaction in the reaction mixture, and finally the purified product was freeze-dried Get G5.NH 2 -FI;

[0068] (2) Dissolve 3.7mg FA and 9.3mg EDC in 3mL DMSO solution, stir magnetically for 3 hours at room temperature to activate the γ-carboxyl group of FA, and gradually add the resulting reaction solution dropwise to 12mL containing 30mg G5.NH 2 -FI in the DMSO solution, under the condition of room temperature under strong magnetic stirring, after 3 days, the product after reaction was successively washed in PBS buffer (3 ...

Embodiment 3

[0070] (1) Configure a mixture of concentrated nitric acid and concentrated sulfuric acid with a volume ratio of 3:1 (ie HNO 3 / H 2 SO 4 For v / v=3:1), acidify 100-200 mg of multi-walled carbon nanotubes, after 2 hours, filter, wash with water, and finally place the acidified multi-walled carbon nanotubes under vacuum conditions for drying and storage;

[0071] (2) Take 16.68 mg of acidified MWCNTs and disperse them in 12 mL of ultrapure water, then drop 2 mL of DMSO solution containing 12.82 mg of EDC into the aqueous solution of MWCNTs, stir with strong magnetic force, and react for 3 hours; then add dropwise 1 mL containing G5.NH prepared in 4.6mg example 2 2 -The aqueous solution of FI-FA was reacted for 48 hours under strong magnetic stirring to synthesize MWCNT / G5.NH 2 -FI-FA complex, disperse the complex in ultrapure water, centrifuge, disperse and centrifuge again, and repeat this 3 times to remove excess reagents;

[0072] (3) The MWCNT / G5.NH prepared in (2) 2 The...

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Abstract

The invention relates to a method for preparing a poly (amidoamine) dendritic polymer / carbon nanometer tube composite material for cancer cell targeting diagnosis. The method comprises that: a carbon nanometer tube CNT is subjected to acidizing treatment; fluorescein isothiocyanate FI and folic acid FA of the poly(amidoamine) dendritic polymer are modified; a CNT composite is obtained through an EDC chemical bonding method and a carboxyl reaction on the surface of a multi-walled carbon nanometer tube which is subjected to the acidizing treatment; terminal amino and acetyl oxide of the rest dendritic polymer on the surface of the composite are subjected to acetylation reaction to obtain a functional composite carbon nanometer tube with neutral surface charge. The functional carbon nanometer tube can be scattered in a solvent for a long time without agglomeration, and the functional multi-walled carbon nanometer tube has good biocompatibility, can be combined on the cancer cell in a targeting manner, and can be used for early-stage target diagnosis of the cancer cell; the method has the advantages of simple preparation method, mild reaction condition and easy operation, and the used polymer is an environment-friendly polymer material, and has industrial implementation prospect.

Description

technical field [0001] The invention belongs to the field of preparation of carbon nanotube composite materials, in particular to a preparation method of polyamidoamine / carbon nanotube composite materials for cancer cell target diagnosis. Background technique [0002] Recent progress in nanoscience and technology research shows that carbon nanotubes, as a multifunctional platform, are widely used in biomedical fields, such as protein and peptide delivery, drug and gene delivery, medical imaging, and cancer targets. Orientation and treatment, etc. However, the surface energy of carbon nanotubes is very high, and the force between tubes (van der Waals force and electrostatic force) is strong, so that carbon nanotubes are easy to aggregate into large bundles, resulting in poor dispersion of carbon nanotubes. The application of carbon nanotubes in biomedicine is generally based on improving the water solubility and biocompatibility of carbon nanotubes. [0003] At present, the...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08L53/02C08L23/22C08K13/02C08K9/04C08K3/26C08K5/09C08K5/01C08K5/07C08K5/103C08K5/524A61K49/12
Inventor 史向阳沈明武肖仕丽
Owner DONGHUA UNIV
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