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Method for preparing plant pathomycete zymotic fluid produced by lactobacillus

A technology for phytopathogenicity and fermentation broth, applied in the biological field, can solve the problems such as unreported patents, and achieve the effects of simple process, low production cost, and high activity against phytopathogenic fungi

Inactive Publication Date: 2009-09-09
TIANJIN UNIVERSITY OF SCIENCE AND TECHNOLOGY
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At present, no literature or patents have been reported on the fermentation conditions of Lactobacillus to produce anti-phytopathogenic fungal active substances and related technologies and methods for the application of fermented products

Method used

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  • Method for preparing plant pathomycete zymotic fluid produced by lactobacillus

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Experimental program
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Effect test

Embodiment 1

[0024] A method for preparing a lactobacillus-resistant phytopathogenic fungus fermentation liquid, the steps of the preparation method are:

[0025] (1) Preparation of seed fermentation liquid: After activating the Lactobacillus plantarum strain twice with MRS liquid medium, inoculate it in a 250ml Erlenmeyer flask, with a liquid volume of 100ml per bottle, and anaerobic culture at a constant temperature of 37°C for 24-48h to become Seed fermentation broth.

[0026] In this step, the composition of the MRS medium is: peptone 10g, beef extract 10g, yeast extract 5g, Tween-801ml, triammonium citrate 2g, K 2 HPO 4 2g, glucose 20g, sodium acetate 5g, MgSO 4 ·7H 2 O 0.1g, MnSO 4 0.05g, 1.0L water, the pH is 6.4-6.7.

[0027] (2) Preparation of fermented product: In a 7L fermenter equipped with 5L fermentation medium and a neutralizing agent for controlling the pH value of the fermented liquid, insert the above-mentioned seed fermented liquid according to the volume ratio of...

Embodiment 2

[0032] Only the static anaerobic cultivation at 37°C for 48 hours in step 2 was changed to static anaerobic cultivation at 39°C for 48 hours. Others are the same as in Example 1.

[0033] Four kinds of lactobacillus fermented liquids prepared by the preparation method of the present invention below carry out the in vitro antibacterial test (growth rate inhibition method) respectively:

[0034] (1) Centrifuge the fermented liquids of the four kinds of lactobacilli obtained at 14000 r / min and 4° C. for 10 min respectively, and collect the supernatants of the four kinds of lactobacilli respectively.

[0035] (2) Add 1ml of the above four supernatants to the melted 9ml PDA medium respectively, pour the obtained 10ml medium into a 9.0cm diameter sterilized petri dish, make a flat plate, and add sterile medium PDA was used as a control. From the edge of the phytopathogenic fungal colony cultivated for 3 to 6 days, use a puncher to cut into 5mm-diameter bacterial blocks, place them...

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Abstract

The invention relates to a method for preparing plant pathomycete zymotic fluid produced by lactobacillus, which comprises the following steps: (1), the preparation of seed zymotic fluid: bacterial strain of lactobacillus is activated for 1-2 times by MRS fluid nutrient medium, inoculated in a triangular flask, and set aside in the constant temperature of 35-40 DEGC for anaerobic cultivation of 24-48 hours so as to turn into the seed zymotic fluid; (2) the preparation of leavening: the seed zymotic fluid is put into a fermentation cylinder filled with fermentation medium and neutralizing agent according to 10% of volume ratio to be fermented, and the culture mode is fed-batch fermentation culture, the mixture is set aside for anaerobic cultivation of 40-60 hours in the temperature of 35-39 DEG C, the Ph value is controlled to be 6.4-6.7, after the fermentation is over, the leavening is obtained; (3) the preparation of zymotic fluid finished products: the leavening is filtered so as to wipe off cells, thus obtaining the zymotic fluid of the lactobacillus. The method can stably obtain the leavening of the lactobacillus which has higher biomass and plant resistant pathomycete activity, can be used for preparing disinfectant curing fungal diseases of plants and provides a new resource for the development of natural biological pesticide.

Description

technical field [0001] The invention belongs to the field of biotechnology, and relates to lactobacilli, in particular to a preparation method of a lactobacillus-resistant plant pathogenic fungus fermentation liquid. Background technique [0002] Worldwide, agricultural production causes huge losses every year due to pests and weeds, of which 80% are caused by fungi. For a long time, people have mainly used chemical pesticides to control crop diseases, insect pests and weeds to ensure stable and high yield of crops. However, the extensive use of chemical pesticides not only pollutes the soil, water and the atmosphere, but also the residues of chemical pesticides in agricultural by-products will directly endanger human health and survival; at the same time, due to the long-term, large-scale and repeated use of chemical pesticides , an increasing number of phytopathogenic fungi have developed resistance to chemically synthesized antifungal agents and antibiotics. Therefore, ...

Claims

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Application Information

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IPC IPC(8): C12P1/04A01N63/02A01P3/00C12R1/25C12R1/245C12R1/23C12R1/225
Inventor 戚薇王海宽张和平王晨颜虎孙天松孙志宏刘文俊
Owner TIANJIN UNIVERSITY OF SCIENCE AND TECHNOLOGY
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