Contamination polyphase metabolism germiculture and product collection interface device used for analyzer

Abstract

The invention relates to a contamination polyphase metabolism germiculture and product collection interface device used for an analyzer, aiming at solving the problem of on-line detection combined use. The technical proposal is as follows: the contamination polyphase metabolism germiculture and product collection interface device consists of a germiculture device and a cooling dewatering device which are communicated with each other. The germiculture device comprises a bottle body, a bottle cap and a liner tube which is arranged in the bottle body. The bottle cap is provided with two switching valves. Two pipelines are communicated with the inside and the outside of the bottle cap by the switching valves. Two pipelines at the outer end of the bottle cap are provided with a throttle switch. Two pipelines at the inner end of the bottle cap are extended into the liner tube with different lengths. The pipeline with longer extended end is a gas-carrier inlet pipeline, and the pipeline with shorter extended end is a gas-carrier outlet pipeline. A sealing ring is arranged between the bottle body and the bottle cap. The contamination polyphase metabolism germiculture and product collection interface device satisfies the requirements of germiculture and gaseous production collection and also has the function of being convenient for on-line use combined with a subsequent analyzer at the same time, thus facilitating the analytic determination of the product. The contamination polyphase metabolism germiculture and product collection interface device can provide a proper test interface device for studying the biogeochemical characteristics of arsenic and other toxic elements and obtain exact real-time analysis data.

Description

technical field [0001] The invention relates to a bacterial culture interface device for collecting pollutant metabolites used in conjunction with analytical instruments, especially for the generation and collection of gas, liquid and solid multiphase bacterial metabolites of toxic element arsenic, which belongs to chemical detection and analysis technology field. Background technique [0002] The toxic element arsenic is one of the most concerned pollutants in the international environmental field, with a wide range of pollution and high toxicity. Arsenic in the environment can form soluble organic arsenic compounds such as monomethylarsenic (MMA) and dimethylarsenic (DMA) through the biomethylation process and enter the liquid or solid phase. The latest research also shows that bacterial or microbial activity may also convert soluble inorganic arsenic to arsine, AsH 3 , Methylarsenic (CH 3 )AsH 2 , Dimethylarsenic (CH 3 ) 2 AsH and trimethylarsenic (CH 3 ) 3 As and...

AI Technical Summary

Problems solved by technology

However, due to the lack of an online sample collection device for gaseous arsenic, the existing analysis methods have been limited to the analysis of standard samples or the analysis of water-soluble arsenic forms in simple matrices such as water samples, and it is difficult to be applied to the analysis of actual gaseous environmental samples.

As far as the gas chromatographic sampling method of gaseous samples is concerned, the current popular headspace solid-phase microextraction gas chromatographic sampling method is also difficult to work, because the commercial extraction heads on the market are mainly for volatile organic compounds, and for gaseous arsenic or The extraction effect of other organometallic compounds is not good, and it is difficult to meet the analysis requirements of trace gaseous arsenic produced by bacteria or other microbial activities

If a syringe is used to inhale gas to inject samples, it is more difficult, and there is a high risk of gas leakage, and the operability is poor. At the same time, it is impossible to take out all the released gas on site.

Although the conventional bacterial culture vessel can carry out bacterial culture, it is not convenient for on-line detection, and also has the following disadvantages: 1) The bacterial culture device is usually open, and the design purpose is only for the purpose of obtaining the reproduction of bacteria. It is convenient to collect the gaseous products after cultivation; 2) it does not have the interface function for online connection with subsequent analytical instruments; 3) it cannot meet the requirements of pressure resistance and leakage prevention when bacterial culture and instruments are used; 4) gaseous arsenic compounds usually need to be stored at low temperature Due to the fact that a large amount of water vapor will be generated during the bacterial culture process, when the collected gas is introduced into the analytical instrument, the water vapor in the gas phase product will easily freeze at the head of the low-temperature chromatographic column, resulting in blockage of the carrier gas pipeline and damage to the equipment. Blockage and poor air flow will cause the pipeline to burst due to excessive pressure, resulting in the risk of leakage

Images