Solid-state fermentation preparation method of lotus root dietary fiber and products thereof
A solid-state fermentation, dietary fiber technology, applied in food preparation, food science, application and other directions, can solve the problems of loss of effective components of soluble dietary fiber, environmental pollution, etc., and achieve the effect of low price, strong operability, and improvement of physiological functions
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Embodiment 1
[0047] The lotus root dregs produced during the processing of lotus root powder or lotus root juice beverages were selected as the main raw material. After being dried and crushed by a pulverizer, they were passed through a 60-mesh sieve, and distilled water was added according to a material-liquid ratio of 1:1.5, sterilized at 121°C for 20 minutes, and cooled. After cooling, mix in 1.5% Angel rice koji, and saccharify at 28-30°C for 36 hours as a solid culture substrate. Inoculate the preserved strains of Ganoderma lucidum (Ganodermalucidum HG) in a petri dish equipped with an activated medium of potato medium, and after culturing at 28-30°C for 5-6 days, use a sterile puncher to measure the diameter on the petri dish. It is 4 slices of 1cm hyphae, inoculated in a 250ml Erlenmeyer flask filled with 50ml of mother seed medium, and cultured at 28-30°C and 120rpm for 5-6 days to obtain the mother seed; the mother seed medium is potato potato Liquid medium (reference: Zhang Song....
Embodiment 2
[0051] According to the raw material treatment and sterilization method in Example 1, mix in 2% Angel rice koji after cooling, and saccharify at 28-30° C. for 36 hours, as a solid culture substrate.
[0052] The mother seed culture method and the composition of the fermentation medium are the same as those in Example 1. The seed solution is inoculated according to 20% of the weight of the raw material, and cultured at 28-30° C. for 7 days.
[0053] The lotus root dietary fiber is prepared through known activation procedures such as heating and drying, extruding and puffing, and ultrafine pulverization. The yield of insoluble dietary fiber in the product is 50.2g / 100g, and the yield of soluble dietary fiber is 12.0g / 100g.
Embodiment 3
[0055] According to the raw material treatment and sterilization method in Example 1, mix in 1.8% Angel rice koji after cooling, and saccharify at 28-30° C. for 36 hours, as a solid culture substrate.
[0056] The mother seed culture method and the composition of the fermentation medium are the same as those in Example 1. The seed solution is inoculated according to 18% of the weight of the raw material, and cultured at 28-30° C. for 7 days.
[0057] The lotus root dietary fiber is prepared through known activation procedures such as heating and drying, extruding and puffing, and ultrafine pulverization. The yield of insoluble dietary fiber in the product is 50.5g / 100g, and the yield of soluble dietary fiber is 11.4g / 100g.
[0058] Table 1 Plackett-Burman experimental design and measurement data
[0059]
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