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Liposome calcium sulphate composite nano artificial bone, preparation method and use thereof

A liposome and artificial bone technology, applied in the field of medical materials, can solve the problems of rapid degradation and absorption, poor osteoinductive ability, high price, etc., and achieve the effect of long usable time, meeting requirements, and high material strength

Active Publication Date: 2012-11-14
SHANGHAI INST OF ONCOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] However, MIIG also has the following problems in the application process: (1) degradation and absorption are faster
(2) slightly weaker strength
(3) Osteoinductive ability is slightly poor
(4) The price is higher
As a classic drug carrier, liposome has very powerful functions, but there is no report on the preparation of liposomal calcium sulfate / calcium phosphate composite nano-artificial bone by combining nano-liposome technology with bone material preparation

Method used

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  • Liposome calcium sulphate composite nano artificial bone, preparation method and use thereof
  • Liposome calcium sulphate composite nano artificial bone, preparation method and use thereof
  • Liposome calcium sulphate composite nano artificial bone, preparation method and use thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0044] Weigh 15g of lecithin and 12g of cholesterol, dissolve in 50ml of dichloromethane, oscillate evenly to obtain solution I, then put it into an eggplant-shaped bottle, distill under reduced pressure on a rotary evaporator, and feed nitrogen from time to time until the dichloromethane The methane is completely evaporated, and then vacuum-dried at room temperature for 24 hours; weigh 3.0g bone morphogenic protein (BMP) and dissolve it in 70ml PBS (pH=7.4) buffer solution and shake well to obtain the aqueous solution II; then add the above 70ml BMP solution II into the eggplant-shaped bottle , under the conditions of ultrasound to fully hydrate the lipid film, after ultrasound, to obtain liposomes loaded with BMP, freeze-dried as a powder for future use.

[0045] Incubate calcium sulfate dihydrate at 100°C for 5 hours to generate calcium sulfate hemihydrate; mix 80g calcium sulfate hemihydrate, 10g calcium sulfate dihydrate and 10g α-tricalcium phosphate, and grind in a ball ...

Embodiment 2

[0048] Take by weighing carboxymethyl chitosan octadecyl quaternary ammonium salt (weight average molecular weight is 2,000,000) 15g, cholesterol 12g is dissolved in 50ml dichloromethane, shakes evenly to obtain solution I; Take by weighing 3.0g gentamicin to dissolve The aqueous solution II was obtained in 70ml of deionized water; then the above two solutions I and II were blended and emulsified, ultrasonicated for 10 minutes, and put into an eggplant-shaped bottle after forming a stable emulsion, and the dichloromethane was evaporated on a rotary evaporator. The polymer liposome aqueous solution loaded with gentamicin, figure 1 It is the TEM photo of the polymer liposome loaded with gentamicin.

[0049] Keep calcium sulfate dihydrate at 1200°C for 4 hours, take it out, and quench it in an ice bath to form anhydrous calcium sulfate. Grind 100 g of anhydrous calcium sulfate in a ball mill for 12 hours to obtain calcium sulfate powder.

[0050] The diluent is an aqueous soluti...

Embodiment 3

[0052] Take by weighing carboxymethyl chitosan octadecyl quaternary ammonium salt (weight average molecular weight is 1000) 15g, cholesterol 12g, 2.0g carbon nanotubes are dissolved in 50ml dichloromethane, shake to obtain solution I evenly; Weigh 3.0g Dissolve BMP in 70ml deionized water to obtain aqueous solution II; then blend and emulsify the above two solutions I and II, ultrasonicate for 10 minutes, put it into an eggplant-shaped bottle after forming a stable emulsion, and volatilize dichloromethane on a rotary evaporator After cleaning, liposomes co-carrying carbon nanotubes and BMP are obtained, and freeze-dried as a powder for future use.

[0053] Calcium sulfate dihydrate is incubated at 120°C for 8 hours to generate calcium sulfate hemihydrate, and calcium sulfate dihydrate is incubated at 1200°C for 2 hours to generate calcium sulfate anhydrous; 30g calcium sulfate dihydrate, 10g calcium sulfate hemihydrate, and 60g anhydrous sulfuric acid Calcium and 5g of tetraca...

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Abstract

The invention relates to a liposome calcium sulfate composite nanometer artificial boneware, as well as a preparation method and application thereof. The preparation method comprises the steps of well mixing liposome (1 to 40 percent), calcium sulfate powder (40 to 80 percent) and diluent (10 to 50 percent), curing the materials and then preparing a bone graft substitute containing nanometer liposome. The liposome can carry medicine and can modify surface, has two forms, namely solution and powder, and comprises traditional micromolecule liposome and macromolecule liposome having macromolecule characteristics. The invention can provide an artificial bone material which has nanometer reinforcing effects, function of sustaining and controlling medicine release, controllable degradation time, good biocompatibility and mechanical strength, can induce bone growth in vivo, and is beneficial to the healing of defective areas of bones.

Description

technical field [0001] The invention relates to the technical field of medical materials, in particular to a liposomal calcium sulfate composite nano-artificial bone, a preparation method and application. Background technique [0002] The key to the treatment of fractures near the joint is to ensure that after the fracture is reduced and fixed, the patient can perform functional exercises early to maximize the recovery of joint function. Bone grafting at the fractured bone defect can provide mechanical support and fill the gap at the same time, which accelerates the healing of the fracture and reduces the occurrence of complications such as loss of reduction, loose internal fixation, delayed union or even nonunion of the fracture. At present, there are many artificial bones used as bone graft substitutes. According to the composition, they can be divided into hydroxyapatite artificial bone, calcium phosphate artificial bone, and calcium sulfate artificial bone. When used, th...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61L27/44A61L27/54
Inventor 段友容梁晓飞宋娜盛晶吴恒
Owner SHANGHAI INST OF ONCOLOGY
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