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Method for preparing silk fibroin hydrogel

A technology of silk fibroin and hydrogel, which is applied in the field of preparation of silk fibroin hydrogel, can solve the problems of toxicity and reduce the biocompatibility of silk fibroin, and achieve the goal of increasing work efficiency and good biocompatibility Effect

Inactive Publication Date: 2009-08-12
SUZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

As we all know, epoxy compounds have certain toxicity. Although the unreacted epoxy crosslinking agent is removed by water washing, there is still residual epoxy crosslinking agent in the gel, which brings certain toxicity and reduces the viscosity of silk fibroin. Biocompatibility (Journal of Biomedical Engineering, 2007, 24(6): 1309~1313)
Chinese invention patent (CN1172002C) discloses a process for producing L-aspartic acid by silk fibroin immobilized cells, in which epoxy compounds are also used as cross-linking agents to prepare silk fibroin hydrogel, which may produce certain Cytotoxicity

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] The preparation steps of the silk fibroin hydrogel provided by the present embodiment are as follows:

[0017] 1. Put 40 grams of white plant silk into 4 liters of mass concentration of 0.05% aqueous sodium carbonate solution, process it at 98 to 100°C for 0.5 hour, then wash it, put it into 4 liters of mass concentration of 0.05% aqueous sodium carbonate solution again, Treat at 98-100°C for 0.5 hours, repeat 3 times to degumming silk, wash and dry thoroughly to obtain pure silk fiber. Add pure silk fiber to 300 ml of 9.3 mol / L lithium bromide aqueous solution, stir and dissolve at 65±2°C to form a mixed silk fibroin solution;

[0018] 2. Put the resulting mixed silk fibroin solution into a dialysis bag, and dialyze it with deionized water for 3 days to remove impurities such as lithium bromide to obtain a pure silk fibroin solution;

[0019] 3. Adjust the mass concentration of silk fibroin solution to 2%;

[0020] 4. At a room temperature of 20°C, pour the silk fibr...

Embodiment 2

[0023] Put 40 grams of raw silk into 5 liters of sodium carbonate aqueous solution with a mass concentration of 0.2%, treat it at 98-100°C for 2 hours to degumming the silk, wash and dry it fully to obtain pure silk fiber. Put pure silk fiber in 300 ml of lithium bromide aqueous solution with a concentration of 9.3 mol / liter, stir and dissolve at a temperature of 65±2°C to form a mixed silk fibroin solution;

[0024] Put the obtained silk fibroin mixed solution into a dialysis bag, dialyze with deionized water for 3 days, remove impurities such as lithium bromide, and obtain a pure silk fibroin solution;

[0025] Adjust the mass concentration of the silk fibroin solution to 4%;

[0026] At 37°C, pour the silk fibroin solution into a beaker, and treat it with ultrasonic vibration for 400 seconds, and the ultrasonic power is 400 watts;

[0027] Pour the silk fibroin solution treated by ultrasonic vibration into a mold, and leave it at room temperature for 5 minutes to obtain a ...

Embodiment 3

[0029] Put 100 grams of waste silk into 4 liters of neutral soap solution with a mass concentration of 0.05%, degumming at 98-100° C. for 0.5 hours, repeat the treatment 3 times, and obtain pure silk fibroin fibers after sufficient washing. Dissolve the dried pure silk fiber with 100 ml of calcium chloride, water and ethanol solution with a molar ratio of 1:8:2 at 75±5°C to obtain a silk fibroin mixed solution;

[0030] Using cellulose membrane as dialysis material, dialyze the obtained silk fibroin mixed solution with deionized water for 3 days to obtain pure silk fibroin solution;

[0031] Regulate the mass concentration of silk fibroin solution to be 6%;

[0032] In an ice-water bath at 0°C, pour the silk fibroin solution into a beaker, and treat it with ultrasonic vibration for 400 seconds, and the ultrasonic power is 400 watts;

[0033] Pour the silk fibroin solution treated by ultrasonic vibration into a mold, and place it at a temperature of 37° C. for 40 minutes to fo...

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Abstract

The invention discloses a method for preparing fibroin protein hydrogel, comprising the following steps: obtaining fibroin protein solution with mass concentration of 1-30% by degumming, dissolving and dialyzing domestic silkworm silk; carrying out ultrasonic oscillation treatment on the fibroin protein solution at the temperature of 0-60 DEG C with ultrasonic power being 10-2000 watt and treatment time being 30-600 seconds; carrying out standing for 0-6 hours, thus obtaining the fibroin protein hydrogel. With the method of ultrasonic oscillation treatment adopted, no chemical cross linking agent is added and the fibroin protein solution can be turned into the hydrogel in a short time, thus greatly improving work efficiency; meanwhile, the obtained fibroin protein hydrogel features good biocompatibility and adaptability to biological and medical fields such as artificial skin, artificial cartilage, cell culture brackets, bioreactors, enzyme immobilization materials and the like.

Description

technical field [0001] The invention relates to a preparation method of a gel material, in particular to a preparation method of a silk fibroin hydrogel, and the prepared material can be applied to technical fields such as biomedicine, biotechnology, and tissue engineering. Background technique [0002] Silk fibroin is non-toxic, non-irritating, has good biocompatibility, can promote the growth of human cells, and has certain biodegradability. Through comparative studies on various biological materials, it has been shown that silk fibroin has the same properties as collagen Proteins have the same biocompatibility in vivo and in vitro (Biomaterials, 2003, 24(3): 401-416), therefore, silk fibroin is an ideal raw material for manufacturing biomedical materials. [0003] Gel is a physical state between solid and liquid, and has unique properties, especially hydrogel, which is soft, can maintain a certain shape, and maintains a large amount of water in it. It is currently widely ...

Claims

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Application Information

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IPC IPC(8): A61L27/22A61L27/52C12N11/02
Inventor 卢神州李明忠
Owner SUZHOU UNIV
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