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Mushroom polysaccharide mother liquor for producing pesticide preparation and preparation thereof

A technology of lentinan and pesticide preparation, which is applied in the direction of chemicals for biological control, botanical equipment and methods, biocides, etc., to achieve the effects of increased dissolution, high synergy, and expanded control range

Inactive Publication Date: 2009-07-22
BEIJING YOLOO BIO TECH CORP
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, our company uses discarded mushroom sticks to extract lentinan, which has not been reported in China.

Method used

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  • Mushroom polysaccharide mother liquor for producing pesticide preparation and preparation thereof
  • Mushroom polysaccharide mother liquor for producing pesticide preparation and preparation thereof
  • Mushroom polysaccharide mother liquor for producing pesticide preparation and preparation thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] Crush 30kg of shiitake mushroom sticks, pass through a 20-mesh sieve, and put them into the soaking pot, add 60kg of warm water at 75-85°C, soak for 3 hours, stir from time to time, raise the temperature to 90°C, turn off the steam, and continue stirring to obtain the soaking liquid; Add 0.1kg of protease at 40-60°C for 3 hours of enzymatic hydrolysis; heat the soaking solution after enzymolysis at 95-100°C for 5 minutes for inactivation treatment; filter out the residue; pump the soaking solution into the reaction kettle and add 100kg For industrial ethanol, pass saturated cooling salt water through the jacket, start alcohol analysis, stop stirring after stirring for 30 minutes, strictly control the temperature between -15~0°C, and precipitate polysaccharide after alcohol analysis for 2 hours, filter The filtrate is an aqueous ethanol solution, which can be used repeatedly after distillation under reduced pressure. After filtration, 0.24kg lentinan solid is obtained. Th...

Embodiment 2

[0019] Crush 100kg of shiitake mushroom sticks, pass through a 20-mesh sieve, and put them into a soaking pot, add 250kg of warm water at 60-70°C, soak for 2 hours, stir from time to time, heat up to 80-85°C, turn off the steam, and continue stirring to obtain the soaking liquid; temperature Add 0.3kg of protease at 45-55°C for 3 hours of enzymatic hydrolysis; heat the soaking solution at 100°C for 2 minutes for inactivation; filter out the residue; pump the soaking solution into the reaction kettle and add 450kg of industrial Ethanol, saturated cooling saline in the jacket, start alcohol analysis, after stirring for 30 minutes, stop stirring, strictly control the temperature between -10 ~ 0°C, after alcohol analysis for 3 hours, the polysaccharide is precipitated, filtered The filtrate is an aqueous ethanol solution, which can be used repeatedly after distillation under reduced pressure; 0.78kg lentinan solid is obtained after filtration, and the actual measurement contains 51...

Embodiment 3

[0021] Crush 50kg of shiitake mushroom sticks, pass through a 20-mesh sieve, and throw them into a soaking pot, add 150kg of warm water at 70-80°C, soak for 3 hours, stir from time to time, raise the temperature to 90°C, turn off the steam, and continue stirring to obtain a soaking liquid; the temperature is 40°C Add 0.18kg of protease at ~50°C for 3 hours of enzymatic hydrolysis; heat the soaking solution after enzymolysis at 95-100°C for 5 minutes for inactivation; filter out the residue; pump the soaking solution into the reaction kettle and add 150kg of industrial Ethanol, saturated cooling saline in the jacket, start alcohol analysis, after stirring for 30 minutes, stop stirring, strictly control the temperature between -15 ~ 0°C, after alcohol analysis for 4 hours, the polysaccharide is precipitated, filtered The filtrate is an aqueous ethanol solution, which can be used repeatedly after distillation under reduced pressure; 0.4kg lentinan solid is obtained after filtratio...

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Abstract

The invention discloses lentinan mother liquor of high content which is used for producing bio-pesticide formulation and a preparation method thereof. The invention is characterized in that the technical process takes a mushrooms bar as raw material, comprising lentinan mother liquor which is prepared by grinding, soaking, enzymolysis, inactivation, alcohol analysis, and dilution. By using the production process, plenty of resources can be saved, the production cycle can be shortened, the productivity can be increased, the production cost can be lowered and the labor intensity can be reduced. The invention is good for control in the production process and has very high economic value and development potential. The produced lentinan mother liquor has the content of 2-20%, and can be used for producing various single and mixed pesticide formulations containing lentinan, which have significant effect on preventing and treating the plant virus diseases.

Description

technical field [0001] The invention belongs to the field of biological pesticides, and in particular relates to a lentinan mother liquor for producing pesticide preparations and a preparation method thereof. Background technique [0002] Lentinan is a polysaccharide isolated from the edible fungus Lentinus edodes. Lentinan can enhance the body's ability to inhibit various bacteria, viruses and parasites, regulate the stability of the overall internal environment, and restore the patient to a normal state. It is the most suitable treatment for preventing and treating tumor recurrence, exacerbation of AIDS symptoms and immunodeficiency virus carriers Adjuvants and preventive healthcare medicines. The remaining solution of the extracted polysaccharides is rich in amino acids. Lentinan not only has a strong inhibitory and killing effect, but also has obvious effects of disease resistance, yield increase and crop quality improvement. It is used to prevent and control viral di...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/00A01N65/00A01P3/00
Inventor 蒋勤军王明康杨会营刘晓娇王晓辉
Owner BEIJING YOLOO BIO TECH CORP
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