Application of isosulfocyanate compound in preparing medicament for treating leukemia

A technology of ester compound, benzyl isothiocyanate, which is applied in the field of isothiocyanate compounds, can solve the problems of high cost of treatment, lack of access to patients, and inability of bone marrow to pay for treatment.

Inactive Publication Date: 2009-07-08
ARMY MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the high cost of treatment and the limitation of matching sources, many patients still die because they cannot obtain suitable bone marrow or afford expensive treatment fees, and fail to receive timely and effective treatment.

Method used

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  • Application of isosulfocyanate compound in preparing medicament for treating leukemia
  • Application of isosulfocyanate compound in preparing medicament for treating leukemia
  • Application of isosulfocyanate compound in preparing medicament for treating leukemia

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0054] Peripheral blood cells or bone marrow were collected from 6 cases of acute myeloid leukemia (AML), 3 cases of acute lymphoblastic leukemia (ALL) and 3 cases of chronic myeloid leukemia (CML), and mononuclear cells were isolated. At the same time, the bone marrow of 4 normal people was collected and hematopoietic stem cells (CD34 + ). AnnexinV / PI double staining method and flow cytometry were used to detect the occurrence of apoptosis after the cells were treated with five kinds of isothiocyanate compounds in the present invention. The concentrations of the five compounds were: BITC 8 μM, AITC 20 μM, PEITC 8 μM , SFN 40μM, Erucin 20μM,; the action time is 24 hours. See the results of the study Figure 8-1A , Figure 8-2A , Figure 8-3A , Figure 8-4A , Figure 8-5A , Figure 8-1B , Figure 8-2B , Figure 8-3B , Figure 8-4B , Figure 8-5B , Figure 8-1C , Figure 8-2C , Figure 8-3C , Figure 8-4C ,and Figure 8-5C , the results showed that the five isoth...

Embodiment 2

[0056] Sixty female NOD / SCID mice aged 4-5 weeks were selected to establish a xenograft model of NOD / SCID mice with acute leukemia U937 cells. NOD / SCID mice were subcutaneously inoculated with 0.2ml acute leukemia U937 cell suspension (containing about 2×10 cells) on the right back. 63 days after cell inoculation, the mice were randomly divided into control group, BITC treatment group, and PEITC treatment group according to body weight. Mice in the control group were intraperitoneally injected with normal saline; mice in the treatment group were injected intraperitoneally with BITC and PEITC at a dose of 12 μmol / day, 3 times a week for 4 weeks. The results showed that the incidence of tumors in the BITC and PEITC treatment groups were 42.5% and 45%, respectively, while the incidence of tumors in the normal saline control group was 87.5% (see Figure 8-1E and Figure 8-3E ); the tumor weights of BITC and PEITC treatment groups were 1.33±1.90 and 1.30±1.87, and those of the co...

Embodiment 3

[0058] Prepare isothiocyanate capsules by the well-known capsule method of those skilled in the art; Wherein said capsule can be made to contain 50% benzyl isothiocyanate (BITC), benzene isothiocyanate Capsules of ethyl ethyl ester (PEITC), allyl isothiocyanate (AITC), sulforaphane (SFN) or glycoside trierucinate (Erucin) can also increase or decrease isothiocyanate Benzyl ester (BITC), phenylethyl isothiocyanate (PEITC), allyl isothiocyanate (AITC), sulforaphane (SFN) or glycoside trierucin (Erucin) content . Using commercially available isothiocyanate compounds, get 1500 grams of benzyl isothiocyanate (BITC), phenylethyl isothiocyanate (PEITC), allyl isothiocyanate (AITC), Sulforaphane (SFN) or Trierucin (Erucin), 418g soybean oil, 400g gelatin, 400g purified water, 70g beeswax, 10g red iron oxide, 10g black iron oxide, 2g Ethylparaben, 200 grams of glycerin, prepared into capsules according to the known capsule preparation technology, that is: adopting soybean oil and bee...

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Abstract

The invention relates to a new use of isorhodanic ester compound in preparing pharmaceuticals for treatment of leukemia. The isorhodanic ester compound in preparing pharmaceuticals for treatment of leukemia has strong pharmacological action as well as characteristics of broad spectrum, special effect and low toxicity, thereby having good medical prospect. Additionally, the invention can be used for acute leukemia (including acute myelaid leukemia, acute T lymphatic leukemia and acute promyelocytic leukemia, and the like) and chronic leukemia.

Description

technical field [0001] The present invention relates to the use of isothiocyanate compounds, in particular to the application of isothiocyanate compounds in the preparation of medicines for treating leukemia. Background technique [0002] Isothiocyanates are widely found in various cruciferous plants such as broccoli, kale, Brussels sprouts, cauliflower, horseradish and watercress. Cruciferous plants are rich in glucosinolates, which are hydrolyzed by plant myrosinase or gastrointestinal microorganisms to produce biologically active hydrolysis products, namely isothiocyanates. [0003] Isothiocyanate compounds mainly include benzyl isothiocyanate (BITC), phenylethyl isothiocyanate (PEITC), allyl isothiocyanate (AITC), sulforaphane (SFN ), and glycoside trierucinate (Erucin), etc. The molecular formula of benzyl isothiocyanate (BITC) is C 8 h 7 NS, the structural formula is: [0004] ; The molecular formula of phenylethyl isothiocyanate (PEITC) is C 9 h 9 NS, the str...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/26A61P35/00
Inventor 高宁
Owner ARMY MEDICAL UNIV
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