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Method for producing high-purity activity fungal polysaccharide

A technology of fungal polysaccharides and production methods, applied in the field of bioengineering, can solve the problems of low anticancer activity, many ineffective components, and low purity content, and achieve the effects of high extraction efficiency, few ineffective components, and low cost

Inactive Publication Date: 2009-06-17
张红伟
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, water extraction, acid extraction, alkali extraction, and ultrasonic extraction are commonly used for the extraction of fungal polysaccharides, which are cumbersome, high in cost, low in purity, low in anticancer activity, and have many ineffective components. Without purification of ineffective components, they cannot be used directly. Used in the production of injections and liquid products

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] Use the powdered dry matter of the fruiting bodies of large fungi as raw materials, mix with pure water twice the weight of the raw materials, adjust the pH value to 4 and then heat, when the temperature rises to 50 ° C, add papain, pectinase, fiber The complex enzyme composed of suzyme is heated to 60°C after one hour. At this time, the pH value is adjusted to 8 with sodium hydroxide, stirred at 1 revolution per minute, and extracted 4 times, each time for 1 hour, and the 4 times of leaching solution Combined and filtered, concentrated under reduced pressure to 30% of the total volume, precipitated with ethanol, and filtered to obtain a crude product. The supernatant is desalted with an ultrafiltration separation device, and after repeated dilution with water and ultrafiltration operations, the fungal polysaccharide concentrate is obtained, and the concentrate is freeze-dried or spray-dried. The polysaccharide purity reaches more than 98% fungal polysaccharide powder. ...

Embodiment 2

[0017] Use the powdered dry matter of solid mycelium as raw material, mix with pure water 6 times the weight of the above raw material, adjust the pH value to 4 and then heat, when the temperature rises to 40 ° C, add papain, pectinase, fiber The compound enzyme composed of suzyme is heated to 70°C after one hour, at this time, the pH value is adjusted to 7 with sodium hydroxide, stirred at 10 revolutions per minute, leached twice, each time for 4 hours, and the leached solution Combined and filtered, concentrated under reduced pressure to 30% of the total volume, precipitated with ethanol, and filtered to obtain a crude product. The supernatant is desalted with an ultra-filtration separation device, and after repeated dilution with water and ultra-filtration operations, the fungal polysaccharide concentrate is obtained, and the concentrate is freeze-dried or spray-dried to achieve a polysaccharide purity of more than 99.6%. As a raw material, it can be processed into soft and...

Embodiment 3

[0019] Use the powdered dry matter of the mycelium of the submerged fermentation broth as raw material, mix it with pure water 4 times the weight of the above raw material, adjust the pH value to 4 and then heat it, when the temperature rises to 45 ° C, add papain and pectin Composite enzyme composed of enzyme and cellulase, heat up to 65°C after one hour, adjust the pH value to 7.5 with sodium hydroxide at this time, stir at 5 revolutions per minute, extract 3 times, each time for 2.5 hours, 3 times The extracts were combined and filtered, concentrated under reduced pressure to 30% of the total volume, precipitated with ethanol, and filtered to obtain a crude product. The supernatant is desalted with an ultra-filtration separation device, and after repeated dilution with water and ultra-filtration operations, the fungal polysaccharide concentrate is obtained, and the concentrate is freeze-dried or spray-dried to achieve a polysaccharide purity of more than 99.1%. As a raw mat...

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Abstract

The invention discloses a method for producing a high purity active fungi polysaccharide comprising the following steps: using powdery dry materials of large-sized fungi fruiting body, solid mycelium and / or deep fermentation liquid fruiting body and mixing said materials with pure water by 2 to 6 times of said materials, heating after the pH is adjusted to 4, adding compound enzyme formed by papain, pectinase and cellulase when the temperature rises to 40-50 DEG C, the temperature rises to 60-70 DEG C after one hour, adjusting the solution pH by sodium hydroxide to 7-8, stirring the solution with a speed of 1-10 r / min, leaching for 2-4 times and each time for 1-4 hours, combining and filtering the 2-4 time leaching liquid, precipitating the filtrate by ethanol when the filtrate is vacuum concentrated to 300f the total volume, and obtaining crude products after filtration, desalting the supernatant by an ultrafitration separation device, obtaining the fungi polysaccharide concentrated solution after repeated dilution by water and ultrafiltration operation, obtaining the fungi polysaccharide powder with a ploysaccharide purity of more than 98by drying or spray drying the concentrated solution. The production method of the invention has advantages of simple process, low cost and high purity content.

Description

1. Technical field [0001] The invention belongs to the field of bioengineering, and relates to a production method of high-purity active fungal polysaccharides. 2. Technical background [0002] Fungal polysaccharides are known internationally as "Biological Response Modifiers", or BRM for short. Anti-aging, anti-radiation and other effects. High-purity active fungal polysaccharides can be directly processed as raw materials to produce products such as soft and hard capsules, tablets, granules, injections, liquids, and oral liquids. At present, water extraction, acid extraction, alkali extraction, and ultrasonic extraction are commonly used for the extraction of fungal polysaccharides, which are cumbersome, high in cost, low in purity, low in anticancer activity, and have many ineffective components. Without purification of ineffective components, they cannot be used directly. It is used in the production of injections and liquid products. 3. Contents of the invention ...

Claims

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Application Information

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IPC IPC(8): C12P19/04
Inventor 张红伟
Owner 张红伟
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