Single-layer functional film urease biologic sensor chip and production method thereof

Abstract

The invention relates to a method for preparing a urease biosensor chip with a single-layer functional membrane. The method comprises the following steps: a pH-sensitive polypyrrole layer and a pH-sensitive polypyrrole-urease layer which are close to nernstian response are prepared on a first extended grid of an ion-sensitive field effect transistor and a second extended grid of a reference fieldeffect transistor by adopting an electrochemical synthesis method so that the pH-sensitive polypyrrole layer and the pH-sensitive polypyrrole-urease layer are capable of both immobilizing urease carrier materials and pH-sensitive materials so as to be used for detecting the pH value change caused by carbamide in a solution to be tested and catalyzed by the urease. The biosensor is formed by composing the ion-sensitive field effect transistor, the reference field effect transistor and a quasi-reference electrode through a polypyrrole-urease composite layer. In the invention, the problem that acarrier layer and a sensitive layer are separated is solved by applying the polypyrrole layer in the carriers and the sensitive materials; the processing steps and the production cost are reduced; better sensitivity and stability are obtained; a technological platform which is used for studying a field effect transistor type bio-chip is established.

Description

technical field [0001] The invention belongs to the technical field of sensors, and relates to a method for researching the follow-up process of a novel biochip, in particular to a design method for a biofunctional film. Background technique [0002] People have been studying biosensors for more than half a century, and have realized biosensors with various principles and functions. A biosensor mainly includes two parts: a transducer and a biofunctional membrane, and the design of the biofunctional membrane varies with the transducer. Each type of transducer has its own advantages. In comparison, the ion-sensitive field-effect transistor transducer with a semiconductor structure, that is, the ion-sensitive field-effect transistor, has many advantages such as small size, high input impedance and low output impedance, fast response, and all solid state. , environmental monitoring, industrial production and other fields have been widely used. The traditional ion-sensitive fi...

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Problems solved by technology

[0005] In order to solve the problem that the carrier layer and the sensitive layer are separated in the prior art potentiometric urease biosensor, the purpose of the present invention is to use the polypyrrole film to be used for the carrier of biological substances and biological sensitive materials at the same time. For this reason, the present invention proposes a Monolayer functional membrane urease biosensor chip and preparation method

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