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Artificial culture method of zinc-enriched Chinese caterpillar fungus mycelia and culture medium thereof

A technology of Cordyceps militaris mycelium and a cultivation method, applied in the directions of microorganism-based methods, biochemical equipment and methods, fungi, etc., can solve the problems such as the lack of corresponding reports on the cultivation method of zinc-rich Cordyceps militaris mycelium products, Achieve significant immunomodulatory effects, low cost and high capacity

Inactive Publication Date: 2012-03-28
LUDONG UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] At present, there are no corresponding reports on zinc-rich Cordyceps militaris mycelium products and their cultivation methods at home and abroad

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Strain: Cordyceps militaris strain Cordyceps militaris 5.701, which comes from China Common Microorganism Culture Collection and Management Center.

[0033] Test tube slant culture (activation):

[0034] Preparation medium: glucose 10g, peptone 10g, yeast extract 10g, agar powder 10g, water 960g, its pH value is 5.0, and it is divided into test tubes; Cordyceps militaris strains are inserted into the test tube slope under sterile conditions, 15 Cultivate in dark at ~30°C for 5-10 days;

[0035] Test tube liquid culture:

[0036] Preparation medium: 10g of glucose, 10g of peptone, 10g of yeast extract, 970g of water, the pH of which is 5.0, divided into test tubes, and sterilized; insert the activated slant bacteria, 15-30°C, stirring speed 120rpm, Cultivate for 5 to 10 days;

[0037] Triangular flask liquid seed culture:

[0038] Prepare medium: glucose 10g, peptone 10g, yeast extract 10g, water 970g, the pH value is 5.0, divide the prepared medium into 500ml Erlenm...

Embodiment 2

[0048] Strain: Cordyceps militaris strain Cordyceps militaris 5.701, which comes from China Common Microorganism Culture Collection and Management Center.

[0049] Test tube slant culture (activation):

[0050] Preparation medium: glucose 20g, peptone 6g, yeast extract 9g, agar powder 15g, water 950g, its pH value is 6.0, and it is divided into test tubes; Cordyceps militaris strains are inserted into the test tube slope under sterile conditions, 15 Cultivate in dark at ~30°C for 5-10 days;

[0051] Test tube liquid culture:

[0052] Preparation medium: glucose 20g, peptone 6g, yeast extract 9g, water 965g, the pH value is 6.0, packed in test tubes, sterilized; insert the activated slant bacteria, 15 ~ 30 ℃, stirring speed 120rpm, Cultivate for 5 to 10 days;

[0053] Triangular flask liquid seed culture:

[0054] Prepare medium: glucose 20g, peptone 6g, yeast extract 9g, water 965g, its pH value is 6.0, divide the prepared medium into 500ml Erlenmeyer flasks, each bottle c...

Embodiment 3

[0064] Strain: Cordyceps militaris strain Cordyceps militaris 5.701, which comes from China Common Microorganism Culture Collection and Management Center.

[0065] Test tube slant culture (activation):

[0066] Preparation medium: glucose 30g, peptone 5g, yeast extract 5g, agar powder 20g, water 940g, its pH value is 7.5, and it is divided into test tubes; Cordyceps militaris strains are inserted into the test tube slope under sterile conditions, 15 Cultivate in dark at ~30°C for 5-10 days;

[0067] Test tube liquid culture:

[0068] Preparation medium: glucose 30g, peptone 5g, yeast extract 5g, water 960g, the pH value is 7.5, packed in test tubes, sterilized; insert the activated slant bacteria, 15 ~ 30 ℃, stirring speed 120rpm, Cultivate for 5 to 10 days;

[0069] Triangular flask liquid seed culture:

[0070] Prepare medium: glucose 30g, peptone 5g, yeast extract 5g, water 960g, the pH value is 7.5, divide the prepared medium into 500ml Erlenmeyer flasks, each bottle c...

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Abstract

The invention discloses an artificial culture method of Zn-enriched cordyceps militaris mycelia and a culture medium thereof; according to weight percentage, the formulation components of the culture medium comprise: 1.0 to 3.0 percent of glucose, 0.5 to 1.0 percent of peptone, 0.1 to 1.0 percent of yeast extract, 1.0 to 2.0 percent of agar powder and the rest of water, and the pH value of the culture medium is 5.0 to 7.5. The artificial culture method comprises the steps of test tube slant surface strain culture (activation), test tube liquid strain culture, triangular flask liquid seed culture, seeding tank culture, fermenter culture, plate-frame filtration, crashing, drying in vacuum, crashing and leading the mycelia into powder. The operation is simple, the culture period is short, the yield of mycelia is high, no pollution and waste are produced, and the method has the function of supplementing zinc, thus being capable of carrying out industrial production.

Description

Technical field: [0001] The invention relates to a method for artificially cultivating zinc-rich Cordyceps militaris mycelia and a culture medium thereof, belonging to the technical field of pharmacognosy. Background technique: [0002] Cordyceps militaris, also known as Cordyceps militaris, belongs to the Ascomycota subphylum Sclerotinia Sclerotiae Sclerotiae Ergotaceae Cordyceps genus, is one of the precious Chinese medicinal materials in my country. Modern pharmaceutical research has shown that Cordyceps militaris contains nucleosides, polysaccharides, cordycepin, sterols and other components, which are basically the same as Cordyceps sinensis. It has therapeutic effects on diseases such as the immune system, nervous system and cardiovascular system, and has anti-tumor and It has anti-cancer effect and is an ideal substitute of Cordyceps sinensis, which has broad development prospects. [0003] Zinc is a kind of trace element, its content and daily intake in the human bo...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/14C12R1/645
Inventor 程显好孙慧涌盖宇鹏朱林张秋胜高兴喜刘林德
Owner LUDONG UNIVERSITY
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