Continuous flow cell electric amalgamation chip based on silicon structure on insulator

Abstract

The invention discloses a cell-electrofusion chip of continuous flow based on silicone structure of an insulator and a processing technology thereof. The cell-electrofusion chip comprises a shell, a microelectrode array chip fixed in the shell, a sample input and output pipe communicating with the inside and the outside of the shell, and a silicone glass sheet encapsulated on the surface of the shell. The microelectrode array chip consists of a siliceous basal layer, a silicon dioxide insulating layer, a low-resistivity silicon electrode layer and a silicon dioxide protective film in turn from down to up. Using the micro machining technology, a groove is etched on the low-resistivity silicon electrode layer of silicon materials on the insulator till the silicon dioxide insulating layer forms a microelectrode structure. In virtue of microscale and arrangement of microchannel, external electric signals forms gradient field with sufficient intensity and increases cell-electrofusion efficiency. At the same time, a linear type microchannel constructed on the chip contributes for the flow of cell suspension and reduces cell adhesion in flow movement as low as possible. The development of the cell-electrofusion experiment of continuous flow can approximate to provide fusion cells with unlimited quantity.

Description

technical field [0001] The invention relates to a device for electrical fusion of biological cells. Specifically, the present invention relates to a chip that provides cell electrofusion, provides and generates the electric field strength and electric field gradient required for cell queuing, electroporation, and fusion, and relates to the precise control of cells in cell electrofusion and the efficient fusion of cells, suitable for Genetics, animal and plant distant hybrid breeding, developmental biology, drug screening, monoclonal antibody preparation, mammalian cloning and other fields. Background technique [0002] Biological cells can form new cells through fusion, which is of great significance in the basic field of modern biomedical engineering. Cell fusion technology has gone through several stages of development such as biological, chemical and physical induction. In the 1980s, with the development of electronic information technology, cell electrofusion technolog...

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Problems solved by technology

However, the number of integrated microelectrodes in the above patents is small, and high-throughput fusion cannot be achieved. On the other hand, the electric field strength and electric field gradient generated by the microelectrodes are relatively weak, which is relatively weak in terms of precise control of cells.

Chinese patent 200610054121.x is a cell fusion device based on the concept of a chip, but in the design of the chip, the anti-corrosion and anti-oxidation capabilities of the chip are less considered, and there are disadvantages of being easily corroded and oxidized. Sample injection and sample discharge are not conducive to the screening and cultivation of fused cells

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