Extraction method of polysaccharides from morinda officinalis how and application thereof in cigarette
An extraction method and technology of Morinda officinalis, applied in the field of plant polysaccharide extraction, can solve the problems that the extraction and purification of Morinda officinalis polysaccharide has not been reported or taken into account, and achieve the effects of improving the quality of absorbing food, improving the taste, and improving the quality of absorbing food.
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Embodiment 1
[0020] a. Dry the Morinda officinalis officinalis after washing, removing impurities, removing the wood core in the middle of the particles, drying or sun-drying at 60°C, and then crush it to 40 mesh;
[0021] b, take the Morinda officinalis powder obtained in step a, according to water: material (m / m) = 20: 1, carry out leaching extraction under the condition of 70 ℃, the leaching time is 2h;
[0022] c, filter and concentrate the filtrate to 1 / 6 of the volume of the original filtrate;
[0023] d. Add the concentrated solution into absolute ethanol, keep the volume fraction of ethanol at 80%, set aside for 48 hours after mixing, and obtain the crude polysaccharide of Morinda officinalis officinalis after filtering, with a yield of 13.8%
[0024] e, the obtained Morinda officinalis crude polysaccharide of step d is put into water and is redissolved, is mixed with the solution that mass concentration is 5%, then adds the sevag reagent [chloroform: n-butanol (V: V] that volume i...
Embodiment 2
[0029] a. Dry the Morinda officinalis officinalis after washing, removing impurities, removing the wood core in the middle of the particles, drying or sun-drying at 60°C, and then crush it to 40 mesh;
[0030] b, take the Morinda officinalis powder obtained in step a, according to water: material (m / m) = 40: 1, carry out leaching extraction under the condition of 75 ℃, the leaching time is 1.5h;
[0031] c, filter and concentrate the filtrate to 1 / 10 of the volume of the original filtrate;
[0032] d. Add the concentrated solution to absolute ethanol, keep the volume fraction of ethanol at 80%, set aside to settle for 48 hours after mixing, and obtain the crude polysaccharide of Morinda officinalis after filtering, with a yield of 13.3%
[0033] e, the obtained Morinda officinalis crude polysaccharide of step d is put into water and is redissolved, is mixed with the solution that mass concentration is 5%, then adds the sevag reagent [chloroform: n-butanol (V / V) that volume is ...
Embodiment 3
[0038] a. Dry the Morinda officinalis officinalis after washing, removing impurities, removing the wood core in the middle of the particles, drying or sun-drying at 60°C, and then crush it to 40 mesh;
[0039] b. Take the Morinda officinalis powder obtained in step a, and carry out leaching and extraction at 65°C according to water:material=25:1, and the leaching time is 1h;
[0040] C, filter, concentrate filtrate to 1 / 8 of former filtrate volume;
[0041] d. Add the concentrated solution to absolute ethanol, keep the volume fraction of ethanol at 80%, set aside for 48 hours after mixing, and obtain the crude polysaccharide of Morinda officinalis officinalis after filtering, with a yield of 12.9%;
[0042] e, put the obtained Morinda officinalis crude polysaccharide into water for redissolving in step d, and prepare a solution with a mass concentration of 5%, then add the sevag reagent [chloroform: n-butanol (V: V) that is 0.7 times the volume of the crude polysaccharide solu...
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