Method for detecting staphylococcus aureus enterotoxin by nanostructured composite metal

Abstract

The invention discloses a method that detects Staphylococcus aureus enterotoxin by a composite nano metal structure, which comprises the following steps: (1) a substrate is chosen and cleaned, and a polystyrene nano-sphere layer is self-assembled on the substrate; (2) the produced self-assembled polystyrene nano-sphere layer is taken as a die, and a silver film and a gold film are vapor-depositedon the self-assembled layer through a vapor deposition method; (3) the self-assembled polystyrene nano-sphere layer is removed through a Lift-off technique, and the nano metal array structure is obtained after array transformation; (4) the nano metal layer is activated by adopting a non-directional covalent connection method, and a biological molecular film layer that resists SE sheep monoclonal antibodies IgG is formed on the surface of the nano metal structure; (5) extinction spectrum of the nano metal structure is extracted by a spectrograph, and an extinction spectrum peak before the biological combination is obtained; (6) a solution to be detected is dropped on the substrate surface and blow-dried after the reaction, and step (5) is repeated; (7) the extinction spectrum peaks that are obtained before and after step (6) are contrasted, thus determining whether the solution to be detected contains SE. The method that detects the Staphylococcus aureus enterotoxin with the composite nano metal structure does not need the purifying and the marking of the solution to be detected, and can be applied into the quick detection of SE or other toxins in the same type.

Description

technical field [0001] The invention relates to a detection method of staphylococcus aureus enterotoxin, in particular to a method for identifying staphylococcus aureus enterotoxin by utilizing localized surface plasmon resonance of metal nanostructures. Background technique [0002] Staphylococcus aureus enterotoxin, with a molecular weight of 25200-28300, has strong heat resistance and drug resistance. Ordinary heating methods cannot remove the toxin in food, so it can only be detected and controlled in the food control link . The detection of Staphylococcus aureus enterotoxin mainly includes latex agglutination, enzyme-linked immunosorbent assay (ELISA), and polymerase chain reaction (PCR). The double-antibody sandwich ELISA method is the most commonly used method for detecting Staphylococcus aureus enterotoxin. There are three necessary reagents in this assay method: (1) solid-phase antigen or antibody; (2) enzyme-labeled antigen or antibody, It is called "enzyme conju...

AI Technical Summary

Problems solved by technology

[0004] At present, localized surface plasmon resonance cannot be applied to the detection of Staphylococcus aureus enterotoxin. Biomolecular membranes for efficient recognition of toxins

When the metal nanostructure material is gold, although an effective biomolecular film can be formed, the local surface plasmon resonance intensity is very weak, and the signal response is not obvious

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