Quick freezing conservation method for bastard halibut spermatozoon

Abstract

A bastard halibut sperm rapid cryopreservation method relates to a cryopreservation method for marine fish sperm and provides a bastard halibut sperm cryopreservation method with simple operation, rapid preservation and high sperm living rate. The present invention contains diluent, antifreeze agent and the confection of hormone cryoprotective liquid and is preserved in liquid nitrogen. The present invention is characterized in that the diluted natural seawater is used as the diluent for preserving sperms which can not activate sperms; glycol with 14g / 100ml density is adopted as the anti-freeze agent; the diluent and the antifreeze agent are used as the low-temperature hormone cryoprotective liquid for bastard halibut sperms and the low-temperature hormone cryoprotective liquid is precooled in a 4 DEG C thermostatic box; the sperm and the hormone cryoprotective liquid are evenly mixed in an eccentric pipe according to the proportion of 1:1 to 1:3; the eccentric pipe is placed below the port of a liquid nitrogen jar for balancing under -20 DEG C for 1 minutes and then rapidly thrown into the liquid nitrogen for preservation. The present invention is used for bastard halibut sperm rapid cryopreservation.

Description

Technical field: [0001] The invention relates to a method for preserving seawater fish sperm. Background technique [0002] Brown flounder (Paralichthys olivacens), commonly known as flounder, belongs to the order Pleuriformes, the family Flounder, and the genus Flounder, and it is a warm-temperate bottom fish in coastal waters. Its natural distribution area is mainly the Asian sea area along the coast of China, Korea, Japan and Russia. It has strong adaptability, fast growth, large individual, tender and delicious meat, and has the effects of anti-inflammatory and detoxifying, strengthening spleen and replenishing qi, etc. It is one of the valuable and important marine cultured species. Due to the large demand in the international market, from the late 1980s to the early 1990s, brown flounder was caught in large quantities, and the resources were severely damaged. In order to make up for the shortage of resources and meet the needs of the market, in the early 1990s, my co...

AI Technical Summary

Problems solved by technology

[0004] The cryopreservation of brown flounder sperm is related to the protection of this species in my country, but there is still a lack of methods suitable for the rapid cryopreservation of brown flounder sperm

The prior art firstly measures the seminal plasma osmotic pressure (mOsm / kg) with a freezing point osmometer, and prepares sperm diluent according to osmotic pressure. The preserved dilution can only be obtained through repeated experiments; the antifreeze agent uses dimethyl sulfoxide, which is highly toxic to sperm and affects the preservation effect of sperm; the cooling process generally uses a cooling device to cool down. It needs to be equipped with a program cooling device; the storage container is a liquid nitrogen tank