Preparation of phthalandione diethyl artificial antigen

Abstract

The invention relates to a method for preparing an artificial antigen of diethyl phthalate, belonging to the biochemical technical field. The method comprises the following steps that: 4-nitroisophthalic acid is taken as a raw material and generates esterification reaction with n-hexyl alcohol under the catalysis of concentrated sulfuric acid, and then a hapten is prepared by utilization of the ferrous powder reaction method to reduce nitryl on a benzene ring of diethyl phthalate into amido; and the artificial antigen of the diethyl phthalate, namely diethyl phthalate-bovine serum albumin, is prepared by utilization of the diazotization method to perform diazotization on the amido on the hapten and make the hapten combined with bovine serum albumin. The method synthesizes the artificial antigen of the diethyl phthalate, has concise and effective synthetic procedures, can be completely used for immunoassay, provides convenience for future research, and can meet the demand of domestic research of the artificial antigen.

Description

technical field [0001] The invention discloses a preparation method of dihexyl phthalate artificial antigen, which belongs to the technical field of biochemical industry. Background technique [0002] Di-n-Hexyl phthalate (DHP) is a kind of phthalate. Phthalate esters are widely used as plasticizers in the processing and production of plastics, especially in the processing and production of polyvinyl chloride (PVC). Since the phthalate plasticizer and the plastic matrix do not form a covalent bond, but are connected by hydrogen bonds and van der Waals forces, they maintain their independent chemical properties, so when they come into contact with water and oil contained in packaged food , it will dissolve. After research, it was found that phthalate is an environmental hormone substance, which interferes with the production, release, transfer, metabolism, reaction and elimination of normal hormones that maintain homeostasis and regulate growth processes, or cause Adverse ...

AI Technical Summary

Problems solved by technology

At present, the detection of dihexyl phthalate at home and abroad mainly includes high performance liquid chromatography (HPLC), gas chromatography (GC), gas-mass spectrometry (GC-MS), liquid-mass spectrometry (LC -MS), etc., but these methods not only require expensive instruments and equipment, professional operators, but also have relatively high requirements for test materials, and further sample pretreatment is required. exact requirements

In recent years, the research on the immunoassay method of phthalates has been carried out at home and abroad, but there is no report on the enzyme-linked immunoassay for dihexyl phthalate at home and abroad. In order to make up for this gap, it is necessary to prepare phthalates Dihexyl phthalate artificial antigen

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