Applications of naringenin and naringin as signal pathway inhibitor of transforming growth factor-beta 1
A transforming growth factor and signaling pathway technology, applied in the application field of inhibitors in the pharmaceutical field, can solve problems such as lack of ideal drugs
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Embodiment 1
[0044] Example 1. Effect of naringenin on the proliferation of rat hepatic stellate cells and the secretion of cell matrix collagen
[0045] Experimental method: Rat hepatic stellate cells (HSC-T6, Hepatic stellate cell, provided by Professor Friedman of Mount Sinai Medical Center, USA) were cultured in a medium containing 10% fetal bovine serum (US PPA company), 100 U / ml of penicillin and streptomycin RPMI1640 medium (Sigma), 5% CO 2 , 37°C (FIL-TER incubator, Thermo Company, Germany). Digest cells with an abundance of 90% with 0.25% trypsin and adjust the number of cells to 5×10 4 / ml, inoculate 100 μl / well in 96-well culture plate, supplement the culture medium containing different concentrations of naringenin 100 μl / well after the cells adhere overnight, 4-6 duplicate wells for each concentration, continue to culture for 48 hours, remove the culture medium Add 0.5 mg / ml MTT (dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (Sigma company) to 100 μl / well of phosphate...
Embodiment 2
[0047] Embodiment 2. Naringenin is to carbon tetrachloride (CCl 4 )-induced liver fibrosis in mice
[0048] Experimental method: Female balb / c mice (provided by Beijing Weitong Lihua Experimental Animal Company) were subcutaneously injected with 10% CCl 4 (carbon tetrachloride), 0.2ml / mouse, 2 times a week, for 4 consecutive weeks to induce liver fibrosis model. From the second week, the treatment group was given naringenin (Nar) high (100mg / kg), medium (50mg / kg) and low (25mg / kg) three doses by intragastric administration (ig), or intravenous injection (iv) 5mg / kg and subcutaneous injection (sc) 20mg / kg for treatment, another group of mice without fibrosis model was set as normal control mice, 10 mice in each group, administered once a day for 3 consecutive weeks. On the 28th day, blood was collected from the orbital vein for blood index detection, and then the mice were sacrificed, and the liver was removed and weighed. In this example, the MDA, CAT and POD kits were pur...
Embodiment 3
[0050] Example 3. Effect of naringenin on the growth of mouse liver cancer H22 subcutaneous tumor
[0051] Experimental method: After making cell suspension with PBS from mouse liver cancer H22 subcutaneous tumor (provided by Institute of Materia Medica, Chinese Academy of Medical Sciences), the number of cells was adjusted to 5×10 6 / ml, inoculated subcutaneously in the forelimb armpit of female balb / c mice with 0.2ml / only. The next day after tumor inoculation, the treatment group was administered with naringenin (Nar) 200mg / kg, 100mg / kg and 50mg / kg orally (ig) 3 doses or intravenously (iv) 20mg / kg, 10mg / kg and 5mg / kg3 Each dose or subcutaneous injection (sc) 40mg / kg, 20mg / kg and 10mg / kg three doses of treatment, 10 mice in each group, administered once a day, for 2 consecutive weeks. After growing out from the tumor, the tumor volume was measured with a vernier caliper every 2 days, expressed as length×width×0.5 width, and the tumor growth curve was drawn.
[0052] Results...
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