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Method for preparation of bromelin

A technology of bromelain and a manufacturing method, applied in the field of manufacture of bromelain, can solve the problems of large loss of enzyme activity, difficulty in forming industrialized production, low enzyme activity, etc., and achieves reduction in loss of enzyme quality, reduction in enzyme activity, The effect of reducing the loss of enzyme activity

Inactive Publication Date: 2008-12-10
广西美仑生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the enzyme recovery rate is low (about 0.1%), and some products can reach food grade. Due to the addition of tannin in production, the environmental pollution is large
[0006] (3), the organic solvent extraction method needs to input a large amount of organic solvents, the activity of enzyme is not high, and the cost is high
Due to the addition of chemical substances in the extraction process, it is difficult to completely separate them, which will affect the quality of the product, thus limiting the scope of application of the product
[0008] Chinese patent 871000106 provides a new method for extracting bromelain from pineapple processing leftovers. A polymer compound polyacrylic acid is used as a precipitating agent to precipitate the bromelain in the fruit juice, then the precipitate is suspended in water, and the Ph value is adjusted. Remove, obtain enzyme finished product after salting out, the whole production process operation is comparatively complicated, it is difficult to form industrialized production
[0009] Chinese patent 200610043457.6 provides a preparation method of bromelain freeze-dried fraction. The pineapple stem is squeezed to extract the juice and then adsorbed with zinc oxide. The temperature is higher than 30°C. According to the actual factory production experience, at such a high temperature, the pineapple enzyme feed solution has already turned sour and deteriorated, and the enzyme activity is also greatly lost. Continuous operation, difficult to carry out industrial extraction production

Method used

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Examples

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Comparison scheme
Effect test

Embodiment 1

[0038] The manufacturing method of bromelain in this embodiment comprises the following steps: purchase of pineapple stem → cleaning of pineapple stem → pulverization of pineapple stem → squeezing juice → coarse filtration → heat exchange → centrifugation → microfiltration → ultrafiltration → refining → synthesis → Vacuum Freeze Drying → Crushing → Preparation → Packaging → Finished Product. Specifically: select fresh and high-quality old pineapple stems, remove young stems, leaves, and other impurities, put them in the cleaning pool for cleaning, and transport the cleaned pineapple stems to the crusher through a conveyor belt for crushing, crushing them into 3 to 5 small pieces, and then Arrive pulverizer through conveyer belt and carry out pulverization process, be pulverized into the particle of 3.1mm size; The pineapple stalk that pulverizes arrives extruder by conveyer belt and carries out extruding process, and there is feeding trough under extruder, just obtains pineappl...

Embodiment 2

[0040]The manufacturing method of bromelain in this embodiment comprises the following steps: purchase of pineapple stem → cleaning of pineapple stem → pulverization of pineapple stem → squeezing juice → coarse filtration → heat exchange → centrifugation → microfiltration → ultrafiltration → refining → synthesis → Vacuum Freeze Drying → Crushing → Preparation → Packaging → Finished Product. Specifically: select fresh and high-quality old pineapple stems, remove young stems, leaves, and other impurities, put them in the cleaning pool for cleaning, and transport the cleaned pineapple stems to the crusher for crushing through a conveyor belt, breaking them into 5 to 8 small pieces, and then Arrive pulverizer through conveyer belt and carry out pulverizing process, be pulverized into the particle of 4.0mm size; The pineapple stalk that pulverizes arrives extruder and carries out extruding process by conveyer belt, there is feeding groove under extruder, just obtains pineapple enzym...

Embodiment 3

[0042] The manufacturing method of bromelain in this embodiment comprises the following steps: purchase of pineapple stem → cleaning of pineapple stem → pulverization of pineapple stem → squeezing juice → coarse filtration → heat exchange → centrifugation → microfiltration → ultrafiltration → refining → synthesis → Vacuum Freeze Drying → Crushing → Preparation → Packaging → Finished Product. Specifically: select fresh and high-quality old pineapple stems, remove young stems, leaves, and other impurities, put them in the cleaning pool for cleaning, and transport the cleaned pineapple stems to the crusher through a conveyor belt for crushing, crushing them into 8 to 12 small pieces, and then Arrive pulverizer through conveyer belt and carry out pulverization process, be pulverized into the particle of 4.5mm size; The pineapple stalk that pulverizes arrives extruder by conveyer belt and carries out extruding process, there is feeding groove under extruder, just obtains pineapple e...

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Abstract

The method relates to a manufacturing method for bromelin, in particular to a new method for the industrialized extraction of the bromelin with pineapple stems (plant) as a main raw material. The method comprises the following steps of: (1) cleaning pineapple stems; 2) crushing the pineapple stems; (3) pressing and extracting juice; (4) carrying out coarse filtration; (5) carrying out heat exchange; (6) centrifuging; (7) carrying out micro-filtration; (8) carrying out ultrafiltration; (9) refining and synthesizing; (10) vacuum freeze-drying; and (11) crushing, concocting, packaging, and manufacturing a finished product. The manufacturing method for the bromelin can save energy and reduce production cost with an enzyme extraction reclaiming rate up to 0.2 percent, high enzyme purity, high enzyme activity above 3,000G DU / g and good stability.

Description

technical field [0001] The invention relates to a method for producing bromelain, in particular to a new method for industrially extracting bromelain with pineapple stems (plants) as the main raw material. Background technique [0002] Bromelain is a kind of proteolytic enzyme, which is a pure natural plant protease produced by biotechnology from the stems, leaves, fruits, fruit stalks, and peels of pineapple plants; bromelain is rich in pineapple aroma, light yellow to brown Yellow powder; bromelain has a strong effect on decomposing fibrin and blood clots, can enhance the absorption of drugs in medical treatment, and can assist in the treatment of cardiovascular and cerebrovascular diseases, tumors and inflammation; bromelain can perform protein hydrolysis and other biochemical It can also be widely used in medicine and health products, beauty cosmetics, food, biology, leather chemical industry, animal feed and other industries. [0003] Bromelain has been studied for a l...

Claims

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Application Information

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IPC IPC(8): C12N9/50
Inventor 龙晓鹏林森黄忠华
Owner 广西美仑生物科技有限公司
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