Method for hydrolyzing propolis flavonoid glycosideby by naringinase

A kind of gum flavonoid glycosides and naringinase technology, which is applied in the field of naringinase hydrolysis of propolis flavonoid glycosides to achieve the effects of improving antioxidant properties, increasing added value and expanding application scope

Inactive Publication Date: 2011-05-04
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Therefore, the bioavailability of flavonoid glycosides in animals is much lower than that of aglycon-type flavonoids

Method used

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  • Method for hydrolyzing propolis flavonoid glycosideby by naringinase
  • Method for hydrolyzing propolis flavonoid glycosideby by naringinase
  • Method for hydrolyzing propolis flavonoid glycosideby by naringinase

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] Take 100 mg of propolis, dissolve it in 200 mL of distilled water, add β-glucosidase, xylanase, cellulase, pectinase, α-amylase for compound enzyme pretreatment, and the enzyme dosage is 10U / mg, 1500U / mg, respectively. mL, 9U / mL, 50U / mL, 1.5U / mL, pretreatment at pH 5.0, temperature 50°C for 6 hours, adding a certain amount of naringinase for conversion, controlling the enzyme concentration to 30U / mg, pH 4.5 , the temperature is 50°C, the enzymolysis time is 24 hours, and the solution is always kept uniform during the reaction process. After enzymatic hydrolysis, add absolute ethanol to make the final concentration reach 60% to inactivate the enzyme. The flavonoid glycoside rutin in propolis obtained under this enzymolysis condition was reduced from 198.4mg / 100g before enzymolysis to 36.3mg / 100g after enzymolysis, and the hydrolysis rate reached 81.7%. The contents of corticosteroids and kaempferol all increased, respectively by 53.2%, 63.2% and 95.9% (seeing table 1, ...

Embodiment 2

[0026] Take 200mg of propolis, dissolve it in 200mL of distilled water, carry out compound enzyme pretreatment as in Example 1, add a certain amount of naringinase for conversion, control the enzyme concentration to 10U / mg, pH value 4.0, temperature 35°C, enzymolysis time 9 hours , keep the solution uniform throughout the reaction. After enzymatic hydrolysis, add absolute ethanol to make the final concentration reach 60% to inactivate the enzyme. The flavonoid glycoside rutin in propolis obtained under this enzymolysis condition was reduced from 198.4mg / 100g before enzymolysis to 109.7mg / 100g after enzymolysis, and the hydrolysis rate reached 44.7%. The contents of corticosteroids and kaempferol all increased, respectively by 1.8%, 30.2% and 39.3% (seeing table 3, image 3 ).

[0027] Table 3 Contents of five flavonoids in propolis before and after enzymatic hydrolysis (mg / 100g)

[0028]

[0029] The POV of the propolis hydrolyzate on the 9th day was 0.4368%, while the P...

Embodiment 3

[0033] Take 800 mg of propolis, dissolve it in 200 mL of distilled water, carry out compound enzyme pretreatment as in Example 1, add a certain amount of naringinase for conversion, control the enzyme concentration at 5 U / mg, pH value 6.5, temperature at 60°C, and enzymatic hydrolysis time for 6 hours , keep the solution uniform throughout the reaction. After enzymatic hydrolysis, add absolute ethanol to make the final concentration reach 60% to inactivate the enzyme. The flavonoid glycoside rutin in propolis obtained under this enzymolysis condition was reduced from 198.4mg / 100g before enzymolysis to 136.8mg / 100g after enzymolysis, and the hydrolysis rate reached 31.0%. The contents of corticosteroids and kaempferol all increased, respectively by 5.3%, 18.8% and 20.2% (seeing table 5, Figure 5 ).

[0034] Table 5 Contents of five flavonoids in propolis before and after enzymatic hydrolysis (mg / 100g)

[0035]

[0036] The POV of the propolis hydrolyzate on the 9th day w...

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Abstract

The invention provides a method for hydrolyzing propolis flavonoid glycoside by naringinase. The method is as follows: naringinase is adopted to carry out constant-temperature enzymolysis of propolis flavonoid glycoside; propolis flavonoid glycoside substance is hydrolyzed into flavonoid aglycone through enzymolysis; moreover, compared with raw materials, the antioxidation property of the productis reinforced. The content of flavonoid substances in the obtained enzymolysis products can be measured through high-efficiency liquid phase chromatogram, and the result shows that the content of flavonoid glycoside substances is reduced, while the content of flavonoid aglycone substances is obviously increased. The method realizes deep development and utilization of propolis products, and is propitious to increase the added value of bee products and expand the application range of bee products. Moreover, the made propolis enzymolysis products has remarkably increased antioxidation property, and can be better used in the fields of medicine, foodstuff and cosmetics, etc. as compared with raw material propolis.

Description

technical field [0001] The invention belongs to propolis processing technology, and relates to a method for hydrolyzing propolis flavonoid glycosides with naringinase. technical background [0002] Propolis (propolis) is a colloidal solid with an aromatic smell that bees collect resin from plant buds, bark and trunk cracks, and mix it with their mandibular gland secretions and beeswax. Propolis is called "black wax" in the ancient Arabic medical book "Medical Code", and it is also one of the main components of the honeycomb that was first recorded in "Shen Nong's Materia Medica". Propolis has various biological effects such as preventing wound infection, regulating immunity, anti-tumor, lowering blood pressure, lowering blood fat, lowering blood sugar and resisting pathogenic microorganisms. The chemical composition of propolis is complex, containing flavonoids, acids, alcohols, phenols, aldehydes, esters, ethers, alkenes, terpenes, steroids and various amino acids, fatty a...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P17/06
Inventor 胡福良刘亚男李英华
Owner ZHEJIANG UNIV
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