Method for preparing rabies virus antigen

Abstract

The invention provides a method for making rabies virus antigen. The method comprises the following steps that: the antigen gene of rabies virus or the combined expression combination of the antigen gene is respectively cloned in a baculovirus carrier so as to obtain a transfer expression carrier; the transfer expression carrier and baculovirus undergo cotransfection so as to carry out homologous recombination or transposition, thereby obtaining recombined baculovirus; the recombined baculovirus is used to infect insect host and cell; the infected insect host is cultured to express corresponding rabies antigen; and the expressed antigen is ingathered and purified so as to obtain rabies virus antigen. The method adopts a baculovirus expression system to make safe and efficient rabies virus antigen in a domestic silkworm bioreactor; moreover, due to having extremely high safety, the made antigen can be directly used to make injection vaccine and oral vaccine used for animal immunization. The method can substantially reduce the production cost of rabies virus antigen, and has the advantages of safety, high efficiency, less energy consumption and low cost, etc.

Description

Preparation method of rabies virus antigen technical field The invention relates to a method for preparing an antigen, in particular to a method for expressing a rabies virus antigen gene or combined antigen gene in insects by using a recombinant baculovirus, which is used for preparing injection and oral rabies vaccines and belongs to the field of genetic engineering. Background technique Rabies is a zoonotic and fatal encephalomyelitis caused by rabies virus (Rabies Virus, RV) invading the central nervous system. Once the disease occurs, the mortality rate is almost 100%. So far, there is no specific treatment drug, and the only effective way is to prevent it by vaccination. Therefore, large-scale preventive immunization to dogs, cats and wild animals is the fundamental measure to control and eliminate rabies. At present, the rabies vaccines for veterinary use in my country mainly include inactivated vaccines and attenuated vaccines. Although traditional vaccines still ...

AI Technical Summary

Problems solved by technology

Although traditional vaccines still play a dominant role in the prevention and control of rabies, the production costs are high, the immunization period is short, and risk factors such as virus escape during vaccine preparation are difficult to avoid

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