Cultivation method of high yield gluconic acid sodium salt

A technology of sodium gluconate and cultivation method, which is applied in the field of microbial cultivation, can solve the problems of low conversion rate and long fermentation cycle, and achieve the effects of increasing initial sugar concentration, reducing residual sugar, and shortening fermentation cycle

Inactive Publication Date: 2008-11-12
QINGDAO LANGYATAI GRP
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The disadvantage of this method is that the fermentation period is relatively long and the conversion rate is not high.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0009] Embodiment 1: In the production of sodium gluconate, the bacterial strain isolated from a large tank with a fermentation conversion rate of 100% is the starting bacterial strain. After culturing in the activated medium, the bacterial strain with normal growth, strong hyphae, and plump spores is taken. Slope as the object of separation and purification, after 10-10 9 Multiple dilution gradients were incubated at 60°C, 70°C, and 80°C for 5 minutes, and then the strains were spread on a flat plate, and incubated at 40°C and 40% relative humidity for 6 hours. When the spores germinated After being irradiated vertically with a 40W ultraviolet lamp at a distance of 30cm for 1 minute, quickly wrap the veneer with black kraft paper and move it into a constant temperature incubator for 3 days at a constant temperature of 40°C and 40% relative humidity. Select the colonies with larger discolored transparent circles, pick their spores on the slope of the special spore growth mediu...

Embodiment 2

[0012] Embodiment 2: In the production of sodium gluconate, the bacterial strain isolated from a large tank with a fermentation conversion rate of 110% is the starting bacterial strain. After culturing in the activated medium, the bacterial strain with normal growth, strong hyphae, and plump spores is taken. Slope as the object of separation and purification, after 10-10 9 For multiple dilution gradients, incubate at 60°C, 70°C and 80°C for 5 minutes, then spread the bacteria on a flat plate, and cultivate at a constant temperature of 40°C and 40% relative humidity for 8 hours. When the spores germinate After 2 minutes of vertical irradiation with a 40W ultraviolet lamp at a distance of 30cm, quickly wrap the veneer with black kraft paper and move it into a constant temperature incubator for 4 days at 40°C and 40% relative humidity. When black spores grow out of the center of the colony, Select the colonies with larger discoloration transparent circles, pick their spores on th...

Embodiment 3

[0015] Embodiment 3: In the production of sodium gluconate, the bacterial strain isolated from a large tank with a fermentation conversion rate of 120% is the starting bacterial strain. After culturing in the activated medium, the bacterial strain with normal growth, strong hyphae, and plump spores is taken. Slope as the object of separation and purification, after 10-10 9 For multiple dilution gradients, incubate at 60°C, 70°C and 80°C for 5 minutes, then spread the bacteria on a flat plate, and cultivate at a constant temperature of 40°C and 40% relative humidity for 10 hours. When the spores germinate After 3 minutes of vertical irradiation with a 40W ultraviolet lamp at a distance of 30cm, quickly wrap the veneer with black kraft paper and move it into a constant temperature incubator for 5 days at 40°C and 40% relative humidity. When black spores grow out of the center of the colony, Select colonies with larger discoloration transparent circles, pick their spores on the s...

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PUM

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Abstract

The invention discloses a method for cultivating high-yield sodium gluconate strains. The method comprises the following steps that: a strain obtained from the separation in sodium gluconate production is taken as an original strain; after the original strain is cultivated by an activation culture medium, an inclined plane of the original strain, which is normal in growth, healthy in mycelium and plump in spore, is taken as an object for separation and purification; after heat preservation treatment, plate spreading is carried out to the inclined plane; after constant temperature cultivation and ultraviolet irradiation, the inclined plane is bound up with kraft paper and then receives constant temperature cultivation again; and a bacterial colony with a big allochroic transparent ring is selected to be cultivated into mature plump spores, and then the high-conversion sodium gluconate strains are screened out via a shaking table. The strains of the invention have the glucose reducing rate reaching 2.5 percent per hour at peak time, have the conversion rate reaching over 117 percent in fermentation tank production, and have the extraction rate reaching over 95 percent within the fermentation period of 14 h. The method raises initial glucose concentration, shortens fermentation period, and reduces residual glucose as well as production cost.

Description

technical field [0001] The invention relates to a method for cultivating microorganisms. Background technique [0002] At present, the preparation process of sodium gluconate is to use penicillin or aspergillus, adopt the aeration and stirring submerged fermentation technology, the fermentation time is 48-60 hours, and the conversion rate is 90%. The disadvantage of this method is that the fermentation period is relatively long and the conversion rate is not high. Contents of the invention [0003] The task of the present invention is to provide a method for cultivating high-yielding sodium gluconate bacterial strains. The bacterial strains cultivated by this method have the advantages of high sugar-acid conversion rate and short acid production cycle. [0004] Its technical solutions are: [0005] A method for cultivating high-yield sodium gluconate strains, using the strains isolated from large tanks with a fermentation conversion rate of 100-120% in the production of ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14C12Q1/04C12R1/645
Inventor 李悦明张希铭徐建春李霞孙慧彬王道会薛元刚迟慎丽
Owner QINGDAO LANGYATAI GRP
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