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Composition containing fucoidan or fucoidan hydrolysate and immunopotentiating material

A fucoidan and hydrolyzate technology, which is applied in the directions of pharmaceutical combinations, plant raw materials, medical preparations containing active ingredients, etc., to achieve the effects of simple manufacture, synergistic enhancement of immune function regulation or immune activation, and easy handling

Inactive Publication Date: 2008-10-08
SUNTORY HLDG LTD +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

For example, chemically synthesized fucose-containing oligosaccharides have been reported so far (Non-Patent Documents 1, 2, and 3), but since these substances are produced by organic synthesis reactions, they are not preferably used in foods, etc.

Method used

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  • Composition containing fucoidan or fucoidan hydrolysate and immunopotentiating material
  • Composition containing fucoidan or fucoidan hydrolysate and immunopotentiating material
  • Composition containing fucoidan or fucoidan hydrolysate and immunopotentiating material

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0171] Preparation of fucoidan fraction

[0172] Add 1000ml of distilled water to 100g of mozuku algae in Okinawa, Japan, and extract at 100°C for 1 hour. After the obtained extract was cooled, suction filtration, electrodialysis (desalting) and freeze-drying were performed to obtain 2 g of fucoidan fraction. Make the fucoidan contain 2N H 2 SO 4 The aqueous solution at 100°C was hydrolyzed for 1 hour, the obtained aqueous solution was neutralized with 2N NaOH, and a sample for analysis of monosaccharides was prepared by labeling with ABEE fluorescence. It was confirmed that the composition of the sugar was as follows: SFuc (fucose sulfate): GlcA (glucuronic acid): Fuc (fucose): Xyl (xylose) = 49.3: 4.9: 12.1: 1 ( figure 1 ).

[0173] Chromatographic column: Cosmosil C18 AR-II (4.6mmφ×250mm)

[0174] Mobile phase: 0.2M potassium borate buffer containing 10% acetonitrile

[0175] Flow rate: 1.0ml / min

[0176] Temperature: 45°C

[0177] Detection: fluorescence detector (...

Embodiment 2

[0179] Production of fucoidan hydrolyzate and determination of immune function regulation

[0180] The hydrolyzate of fucoidan was obtained under various conditions described below, and the immune function regulation effect was investigated using the composition of the hydrolyzate and lactic acid bacteria.

[0181] The Effect of Hydrolyzed Acid Concentration on the Modulatory Activity of Immune Function

[0182] The fucoidan obtained in Example 1 was hydrolyzed in 0.1, 0.2, 0.3, 0.4, 0.5, 1.0, 2.0, 4.0, 5.0, 6.0N HCl (80° C.) for 1 hour, and then 0.1, 0.2, After neutralization with 0.3, 0.4, 0.5, 1.0, 2.0, 4.0, 5.0, 6.0N NaOH, desalting by electrodialysis and freeze-drying, the hydrolyzate of fucoidan is obtained. The hydrolyzate was added at a concentration of 100 μg / ml to splenocytes (5 × 10 6 cells / ml). After 30 minutes, add 0.1 μg / ml lactic acid bacteria ( Lactobacillus pentosus ; FERM ABP-10028), cultivated for 24 hours, recovered the culture supernatant, and the p...

Embodiment 3

[0191] Regulatory Effects of Refined Fucoidan Hydrolyzate on Immune Function

[0192] Add 100ml of 2N HCl to 1g of fucoidan, and perform acid hydrolysis at 80°C for 1 hour. It was then neutralized with 1N NaOH. The resulting reaction solution was desalted by gel filtration (biogel P-6 (Bio-Rad)), and freeze-dried to obtain a component (oligosaccharide component) containing a fucoidan-oligosaccharide mixture. The resulting fucoidan-oligosaccharide mixture was subjected to chromatography using an anion exchange resin (Tosoh Corporation) activated with formic acid. As a result, the oligosaccharide fraction was separated into a fraction (Fr.B) containing oligosaccharides containing no sulfate groups obtained by elution with water, and a fraction containing oligosaccharides containing a large number of sulfate groups obtained by elution with 2N HCl. Components of sugars (sulfated sugar components). The sulfated sugar fraction was passed through gel filtration (Biogel P-6) and se...

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Abstract

Materials are provided that potentiate an immuno-stimulating activity of materials such as lactic acid bacteria. Such materials contribute to reducing the use of immuno-stimulating materials. It also contributes to shortening the time taken to select lactic acid bacteria that have a strong immuno-stimulating activity and preferred fermentation characteristics. For the purposes, fucoidan or a product obtained by acid hydrolyzing fucoidan (or oligosaccharides derived from it) is used in combination with an immuno-stimulating material. This combination synergistically potentiates the immunomodulatory activities or immuno-stimulating activities of the ingredients.

Description

technical field [0001] The present invention relates to a combination containing fucoidan or a fucoidan hydrolyzate and an immunostimulatory material, which can be used in food and drink, pharmaceuticals, health food, functional food, cosmetics, etc. for the purpose of enhancing immunity, regulating immune function, etc. thing. Background technique [0002] immune boosting material [0003] In recent years, materials having an immunostimulating effect have attracted attention in the field of health foods, and a large number of health foods containing these materials have been developed. It is used for the recovery of immunity weakened by aging, mental stress, disease, etc. This material contains microorganisms such as lactic acid bacteria (lactococcus, lactobacillus), yeast, fungi; [0004] Lactic acid bacteria [0005] Among the above-mentioned immunostimulatory materials, the immunostimulatory effect of lactic acid bacteria has attracted attention, and some lactic acid...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/702A61K8/00A61K45/00A61P37/02A61K35/747A61K36/06A61K39/00
CPCA61Q19/00A23L1/3002A23L1/337A23V2002/00A61K8/60A61K8/606A61K8/975A61K8/99A61K31/7016A61K31/702A61K31/713A61K31/716A61K31/737A61K31/739A61K35/747A61K36/02A61K36/06A61K36/07A61K36/074A61K39/00A61K39/39A61K45/06A61K2800/70A23L33/105A23L17/60A61K8/9711A61K8/9728A61P37/02A61P37/04A61K2300/00A23V2200/324A23V2250/202
Inventor 野中裕司安元健直木秀夫楠元俊英
Owner SUNTORY HLDG LTD
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