Method for detecting enoxacin in biological body fluid with Mn doping ZnS quantum point room temperature phosphorescent

Abstract

The invention relates to a method of a Mn doped ZnS quantum dots room temperature phosphorescence detecting an enoxacin in the biological body fluids. The invention uses the room temperature phosphorescence properties of the Mn doped ZnS quantum dots, and provides a simple, fast, economical, keen and highly selective method to detect the enoxacin in the biology body fluids; the Mn doped ZnS quantum dots are dissolved to be a solution, a urine sample or a serum sample is diluted into 30, 50 or 80 times for detecting the linear equation of the enoxacin, whose linear range is 0.2-7.2 mu mol / L, and detection limit is 58.6 nmol / L; when the method is used to detect the enoxacin in the biological body fluids, an oxygen scavenger and a inducer are not need to add, and the background fluorescence and the interference of scattered light can be avoided. Simultaneously, due to the high selectivity of the method, when the enoxacin is detected in the biological body fluids, a complex sample pretreatment process is not needed.

Description

【Technical field】: [0001] The invention relates to aqueous phase synthesis of Mn-doped ZnS quantum dots for room-temperature phosphorescence detection of enoxacin in biological fluids, and belongs to the technical field of biological analysis and detection. 【Background technique】: [0002] Quantum dots are mainly semiconductor nanoparticles composed of II-VI group elements or III-V group elements. Compared with organic fluorescent dyes, the photoluminescent properties of quantum dots are very superior: long-range excitation, narrow and symmetrical emission peaks, large Stokes shift, high quantum yield, and difficult photolysis. The fluorescent properties of quantum dots have been widely used to detect various ions, small molecules and biological macromolecules. However, the phosphorescent properties of quantum dots and their applications in analytical detection have received less attention. [0003] Room temperature phosphorescence has many advantages over fluorescence ana...

AI Technical Summary

Problems solved by technology

[0005] At present, the methods commonly used to separate and detect enoxacin in complex samples are various combined techniques, but they need to derivatize the analyte, the operation is cumbersome, and the derivatizers used are generally toxic, and the instruments are relatively expensive

Although the spectral analysis method is simple, fast, economical and sensitive, there will be interference from background fluorescence and scattered light.