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Method for preparing bio-diesel by immobilized lipase-alcohol perselective membrane bioreactor

A technology for immobilizing lipase and bioreactor, which is applied in the directions of enzyme production/bioreactor, biochemical equipment and method, liquid production bioreactor, etc. The effect of simplifying the process and avoiding the inhibition of enzyme activity

Inactive Publication Date: 2008-09-03
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

It can be seen that there are obvious deficiencies in the existing technology in terms of avoiding substrate inhibition and reducing enzyme activity while realizing continuous production and simplifying the production process.

Method used

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  • Method for preparing bio-diesel by immobilized lipase-alcohol perselective membrane bioreactor

Examples

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Effect test

Embodiment 1

[0018] The lipase derived from Candida antarctica was immobilized on the surface of polydimethylsiloxane hollow fiber membrane by physical adsorption to make a membrane module, which was connected to an enzyme-membrane bioreactor. Inject 5L of fish oil into the grease storage tank 1, and go through the membrane module 3 shell side through the pump 2, with a flow rate of 4L / h; the methanol in the low-carbon alcohol storage tank 9 goes through the membrane module 3 tube side through the pump 8, with a flow rate of 20L / h. The yield of biodiesel is about 95% after 2 hours of circulation.

Embodiment 2

[0020] Lipase derived from Rhizomucor miehei was immobilized on the surface of polytrimethylsilapropyne hollow fiber membrane by physical adsorption to make a membrane module, which was connected to an enzyme-membrane bioreactor. Inject 5L of lard into the grease storage tank 1, and go through the membrane module 3 shell side through the pump 2, with a flow rate of 4L / h; the methanol in the low-carbon alcohol storage tank 9 is passed through the membrane module 3 tube side through the pump 8, with a flow rate of 20L / h . The yield of biodiesel is about 96% after 4h circulation.

Embodiment 3

[0022] Lipase derived from Thermomyces lanuginosus was immobilized on the surface of chitosan hollow fiber membrane by physical adsorption to make a membrane module, which was connected to an enzyme-membrane bioreactor. Inject 5L of castor oil into the grease storage tank 1, and go through the pump 2 to the shell side of the membrane module 3 at a flow rate of 5L / h; the ethanol in the low-carbon alcohol storage tank 9 is fed to the membrane module 3 tube side by the pump 7 at a flow rate of 100L / h . The yield of biodiesel is about 100% after circulation for 10 hours.

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Abstract

The invention discloses a biological preparation by immobilized lipase-alcohol permeable membrane biological reactor. The process is that lipase is immobilized on surface of alcohol permeable membrane made of hollow fiber by physical absorption, membrane member of immobilized lipase is prepared, then assembled into enzyme-membrane reactor. Oil passes through shell side of membrane member, and low carbon alcohol passes through tube pass of the same, then fatifies them into biological diesel under catalysis of immobilized lipase. Low carbon alcohol selectively permeates hollow fibers to provide low carbon alcohol needed in preparation of biological diesel, so that inhibitory action towards enzyme activity caused by bottom articles is effectively avoided, and biological diesel is efficiently prepared. Moreover, solvent washing and batchwise charging is avoided, which meets the need of green chemistry, and reduces cost, simplify process, realizes continuous production.

Description

technical field [0001] The invention belongs to the technical field of bioenergy. In particular, it relates to a method for preparing biodiesel with an immobilized lipase polymer membrane capable of selectively permeating low-carbon alcohols and a bioreactor thereof. Background technique [0002] Biodiesel is a long-chain fatty acid methyl ester (or ethyl ester, etc.) synthesized by transesterification from renewable oil raw materials. It is an environmentally friendly fuel oil that can replace diesel. The preparation of biodiesel by immobilized enzyme has the advantages of high efficiency, low energy consumption (mild reaction conditions), good selectivity, small amount of alcohol, less by-products, and environmental friendliness (no need for waste liquid treatment). Lipase can efficiently catalyze the transesterification reaction between alcohols and fatty acid glycerides, but the price of lipase is high in the immobilized enzyme method, the adsorption of by-product glyce...

Claims

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Application Information

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IPC IPC(8): C10G3/00C12M1/40
CPCC12M29/04C12M21/12Y02E50/13C12M21/18C12M29/02Y02E50/10Y02P30/20
Inventor 徐志康万灵书黄小军
Owner ZHEJIANG UNIV
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