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Technique for producing toasted garlic polysaccharides and allinase by using toasted garlic as raw material

A garlic polysaccharide and production process technology, applied in the direction of lyase, can solve the problem of no industrial production of alliinase, etc., and achieve high production and application value, simple method, and high recovery of enzyme activity

Inactive Publication Date: 2008-09-03
JINAN UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Although alliinase has great practical and potential utilization value, there is no report on the industrial production of alliinase

Method used

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  • Technique for producing toasted garlic polysaccharides and allinase by using toasted garlic as raw material

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Using fresh garlic as raw material, the method for jointly preparing alliinase and garlic polysaccharide is specifically carried out according to the following steps:

[0031] The first step is pretreatment: put fresh garlic cloves in drinking water whose quality is about 6 times its mass, soak for 2 hours, wash and peel in running water, control dry clear water, put it in a beater and crush it into garlic pulp with a particle size of 200 mesh . Garlic pulp is squeezed and filtered with a plate filter. Wash the filter residue with water for 2-3 times, combine the filtrate, and take the filtrate for later use.

[0032] The second step is to separate alliinase: add food-grade hydrochloric acid dropwise to the obtained garlic extract, and stir rapidly to reduce the pH of the extract to 3.0, centrifuge, and dissolve the obtained precipitate with a phosphate buffer solution with a pH of 6. , using various ultrafiltration membranes with a molecular weight cut-off of 30,000 ...

Embodiment 2

[0036] Using fresh garlic as raw material, the method for jointly preparing alliinase and garlic polysaccharide is specifically carried out according to the following steps:

[0037] The first step is pretreatment: put fresh garlic cloves in drinking water whose quality is about 4 times its mass, soak for 2 hours, wash and peel in running water, control dry clear water, put it on a beater and crush it into garlic pulp with a particle size of 300 mesh. Garlic pulp is squeezed and filtered with a plate filter. Wash the filter residue with water for 2-3 times, combine the filtrate, and take the filtrate for later use.

[0038] The second step is to separate alliinase: add phosphoric acid dropwise to the obtained garlic extract, and stir rapidly to reduce the pH of the extract to 5.0, centrifuge, and the obtained precipitate is dissolved in a phosphate buffer solution with a pH of 6. Various ultrafiltration membranes with a molecular weight cut-off of 30,000 to 60,000 Daltons are...

Embodiment 3

[0042] Using fresh garlic as raw material, the method for jointly preparing alliinase and garlic polysaccharide is specifically carried out according to the following steps:

[0043] The first step is pretreatment: put fresh garlic cloves in drinking water whose quality is about 3 times its mass, soak for 2 hours, wash and peel in running water, control dry clear water, put it on a beater and crush it into garlic pulp with a particle size of 300 mesh. Garlic pulp is squeezed and filtered with a plate filter. Wash the filter residue with water for 2-3 times, combine the filtrate, and take the filtrate for later use.

[0044] The second step is to separate alliinase: add citric acid or lactic acid drop by drop to the garlic extract, and stir quickly to reduce the pH of the extract to 4, centrifuge, and dissolve the obtained precipitate with a phosphate buffer solution with a pH of 7 , using various ultrafiltration membranes with a molecular weight cut-off of 30,000 to 60,000 Da...

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Abstract

The fresh garlic is used as raw material and subjected to cleaning, roguing, crushing and juicing, filtering, adjusting the pH value of the juice, centrifugating to obtain raw alliinase; then dissolving, ultrafitering, freeze drying to produce alliinase product of high purity. The centrifugated supernatant is treated by cation exchange resin column and anion exchange resin column to obtain fine liquid of alliinase and then the garlic polysaccharide product of high purity is obtained by concentrating, pneumatic conveying drying. The invention uses raw material of the same batch to produce two active materials such as alliinase, garlic polysaccharide. The utilization factor of garlic resource is greatly increased and the environment pollution resulted from the single product is reduced and the manufacturing cost is reduced, the benefit for producing garlic polysaccharide or alliinase is greatly increased.

Description

technical field [0001] The invention belongs to the field of extraction, separation and purification of natural products, and in particular relates to a method for jointly preparing alliinase and garlic polysaccharide by using fresh garlic as a raw material. Background technique [0002] Garlic (Allium sativum L.) polysaccharide accounts for 70%-80% of the dry matter of garlic, is the component with the highest content in garlic, and belongs to fructan. CN1239719A reports that the molecular weight of garlic polysaccharide is 3500, and the content is 80% miscellaneous fructan, which is expected to replace the precious Achyranthes bidentata polysaccharide for medicine. Studies have confirmed that fructan has many physiological functions such as controlling blood lipids, lowering blood sugar, promoting mineral absorption, proliferating intestinal bifidobacteria, preventing constipation and treating obesity. Recent studies have also confirmed that garlic polysaccharide has the ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/00C12N9/88
Inventor 黄雪松孔祥智
Owner JINAN UNIVERSITY
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