Black edible fungus health care oral liquid and method of preparing the same
A technology of black fungus oral liquid and black fungus oral liquid, applied in food preparation, application, food science, etc., to achieve the effects of full energy, keeping a clear mind, and increasing production
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Embodiment 1
[0025] Embodiment 1: Prepare black fungus for oral administration according to the following steps:
[0026] ① Homogenize the harvested fresh Gastrodia elata;
[0027] ②Pick a soybean-sized piece from the black fungus parent to the center of the slope, and culture it at 25°C for 6 days;
[0028] ③Transfer the cultured slant strains to the shake flask culture medium, one slant to one bottle. 500ml Erlenmeyer bottle with 200ml of liquid, cultured on a rotary shaker, rotating speed 180r / min, cultured at 25-30°C for 4-6 days;
[0029] ④ Inoculate the cultivated shake flask seeds into the seed tank for cultivation. The stirring speed was 150r / min, the fermentation temperature was 27°C, the ventilation rate was 2L / L fermentation broth min, the tank pressure was 0.2MPa, and the cultivation was carried out for 4 days.
[0030] ⑤ adding the pretreated Gastrodia elata homogenate to the fermentation base medium, and the addition amount of the Gastrodia elata homogenate is 6%. After s...
Embodiment 2
[0033] Embodiment 2: Prepare black fungus for oral administration according to the following steps:
[0034] ① Homogenize the harvested fresh Gastrodia elata;
[0035] ②Pick a soybean-sized piece from the black fungus parent to the center of the slope, and culture it at 26°C for 4 days;
[0036] ③Transfer the cultured slant strains to the shaker flask culture medium, one slant to one bottle; 500ml triangular flask with 200ml liquid volume, culture on a rotary shaker, rotation speed 150r / min, culture temperature 27°C for 6 days ;
[0037] ④ Inoculate the cultivated shake flask seeds into the seed tank for cultivation; the stirring speed is 180r / min, the fermentation temperature is 25°C, the ventilation rate is 1L / L fermentation broth min, the tank pressure is 0.25MPa, and cultured for 2 days;
[0038] ⑤Add the pre-homogenized Gastrodia elata into the fermentation basal medium, and the addition amount of Gastrodia elata pulp is 8%. The cultivated seeds were put into the ferme...
Embodiment 3
[0041] Embodiment 3 prepares black fungus oral administration according to the following steps:
[0042] ① Homogenize the harvested fresh Gastrodia elata.
[0043] ②Pick a soybean-sized piece from the female black fungus to the center of the slope, and culture it at 28°C for 5 days.
[0044] ③Transfer the cultured slant strains to the shake flask culture medium, one slant to one bottle. 500ml Erlenmeyer bottle with 150ml liquid volume, cultured on a rotary shaker, rotating speed 200r / min, cultured at 28°C for 3 days;
[0045] ④ Inoculate the cultivated shake flask seeds into the seed tank for cultivation. The stirring speed was 150r / min, the fermentation temperature was 27°C, the ventilation rate was 2L / L fermentation broth min, the tank pressure was 0.3MPa, and the culture was carried out for 4 days.
[0046] ⑤ adding the pre-homogenized Gastrodia elata into the fermentation basal medium, and the addition amount of Gastrodia elata pulp is 10%. Put the cultivated seeds int...
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