Preparation of high-purity animals cartilaginous collagen polypeptides

Abstract

The invention relates to a technological method for preparing collagen polypeptide from raw material of collagen powder by value-raised deep-processing technology, wherein the collagen powder is obtained by extracting chondroitin sulfate from animal cartilage; in particular to a method for preparing highly purified animal cartilage collagen polypeptide in the filed of natural material extraction technology. The method includes the following steps: first, animal cartilage collagen powder is prepared into aqueous solution, after clarification, the aqueous solution is stirred in organic solvent, stood, and extracted to remove lipid components and peculiar smells; second, salt is eluted from lower solution by the sephadex column chromatography filtering method, the eluent is collected, and then heated to 40-55 DEG C, macerozyme is selected to cut pacemaker enzyme under the condition that the collagen powder is stirred, and the eluent is stirred, decolored, filtered, concentrated and dried to obtain the hydrolytic collagen polypeptide. The method has the advantages of simple technological process, easily obtained raw material, high production efficiency, high product quality, good taste, low cost and good chemical properties and is suitable for large-scale industrial production.

Description

Technical field: [0001] The invention relates to a process for preparing collagen polypeptides through high-value deep processing technology for collagen powder obtained after extracting chondroitin sulfate from animal cartilage, in particular to a high-purity animal cartilage in the technical field of natural substance extraction Preparation method of collagen polypeptide. Background technique: [0002] At present, the technology of deepening the preparation of high-quality collagen peptides with collagen powder as raw material is arousing widespread interest. Collagen is the most abundant and widely distributed protein with immunological properties in animals. It widely exists in animal skin, bones, teeth, tendons, ligaments and blood vessels, accounting for 30% of the total protein in mammals. It is widely used in the pharmaceutical industry, biomedical raw materials and food industry, imaging industry, etc. The earliest collagen products produced using leather scraps a...

AI Technical Summary

Problems solved by technology

Chinese patent CN1896262A discloses "technology for producing animal high-calcium powder, chondroitin, and collagen by compound enzyme method". There are the following disadvantages in protein powder or further made into collagen polypeptide: (1) has a strong fishy smell; (2) the color is dark and dark yellow; (3) the salt content is as high as more than 10%; (4) due to the molecular weight distribution is not Uniform, affects digestibility and often has poor organoleptic properties