Animal collagen polypeptide extraction method
A technology of collagen polypeptide and extraction method, which is applied in the field of food chemical industry, can solve problems such as deep color, affecting digestibility, strong fishy smell, etc., and achieve the effect of simple process, excellent product quality and good chemical properties
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Embodiment 1
[0013] Embodiment 1: Shark cartilage collagen powder is first prepared with distilled water and is 20% aqueous solution by weight and is placed in the extraction tank, after the solution is clarified, n-hexane is added according to the weight percentage of 50% of the solution, stirred for 1 hour, and allowed to stand Extract for 6-8 hours; pass the lower layer solution through Sephadex G-10 gel chromatography column, elute with distilled water and collect the eluate; The weight percentage ratio of mixed enzymes is added, and the weight ratio of the mixed enzymes is collagenase: Bacillus subtilis 1.398 neutral protease: Aspergillus terreus 3.942 protease = 1:2:1; then enzyme digestion under constant stirring for 2-4 hours, After enzymatic digestion, add powdered activated carbon according to the weight percentage of 6% cartilage collagen powder, stir and decolorize at 45-51°C for 0.5 hours, filter, vacuum concentrate, and then spray dry to obtain colorless, odorless, fat-free ,...
Embodiment 2
[0014] Embodiment 2: Collagen powder obtained after extracting chondroitin sulfate from pig nasal bone is used as raw material, the steps and method are the same as in Example 1, and the organic solvent is selected as petroleum ether to obtain a product of the same quality.
Embodiment 3
[0015] Embodiment 3: Collagen powder obtained after extracting chondroitin sulfate from bovine cartilage is used as raw material, adopts the process and conditions of embodiment 1, and selects the organic solvent as diethyl ether to obtain a product of the same quality.
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