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Biological technique method for recovering structure and function of erythrocyte

A red blood cell and function technology, applied in the field of cell biology, can solve the problems of repairing and not seeing the charged state structure and function of red blood cell surface, and achieve the effect of restoring structure and function, simple steps, safe and convenient operation

Active Publication Date: 2010-12-08
JINAN UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] So far, the world's research on the influence of the charge state on the surface of red blood cells on its structure and function mainly considers various factors, including the sialic acid residues in the cell membrane due to cell aging, storage time, and artificial digestion with trypsin. In the case of the reduction of the charge on the surface of red blood cells caused by the shedding of bases, the impact on the structure and function of red blood cells and other life states has not been seen, but there is no research on how to adopt relevant technical methods to restore the charged state of the surface of red blood cells so that its structure and function can be repaired.

Method used

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  • Biological technique method for recovering structure and function of erythrocyte
  • Biological technique method for recovering structure and function of erythrocyte

Examples

Experimental program
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Effect test

Embodiment 1

[0022] (1) Prepare aged red blood cell samples. 2ml of venous blood was drawn from young healthy male volunteers with no smoking history and no recent medication history, and anticoagulated with heparin. Wash with isotonic PBS buffer (2000r / min, 5min) 3 times. Put the washed red blood cells (20-fold dilution) on the formed discontinuous density gradient solution, centrifuge at 2700r / min for 30min, take the red blood cells at the bottom layer (ie old red blood cells), wash them three times with PBS (2000r / min, 5min) Suspended in isotonic PBS buffer and stored at 4°C for later use.

[0023] (2) Use vitamin E solution to act on aged red blood cells. Take 0.1ml of aged erythrocytes prepared in (1) and washed, and dilute to 1ml with calf serum 1640 solution containing 10% volume fraction, the concentration is about 6×10 8 pieces / ml. Add 50 μl of vitamin E working solution with a concentration of 160 μg / ml (that is, a solution of vitamin E diluted to a concentration of 160 μg / ml...

Embodiment 2

[0031] (1) Prepare aged red blood cell samples. 2ml of venous blood was drawn from young healthy male volunteers with no smoking history and no recent medication history, and anticoagulated with heparin. Wash with isotonic PBS buffer (2000r / min, 5min) 3 times. Put the washed red blood cells (20-fold dilution) on the formed discontinuous density gradient solution, centrifuge at 2700r / min for 30min, take the red blood cells at the bottom layer (ie old red blood cells), wash them three times with PBS (2000r / min, 5min) Suspended in isotonic PBS buffer and stored at 4°C for later use.

[0032] (2) Use N-acetylcysteine ​​working solution to act on aged red blood cells. Take 0.1ml of the washed aged red blood cells prepared in (1), dilute to 1ml with 1640 solution containing 7% calf serum, the concentration is about 7×10 8 pieces / ml. Then add 50 μl of N-acetylcysteine ​​working solution with a concentration of 140 μg / ml (that is, an aqueous solution of N-acetylcysteine ​​with a c...

Embodiment 3

[0040] (1) Prepare aged red blood cell samples. 2ml of venous blood was drawn from young healthy male volunteers with no smoking history and no recent medication history, and anticoagulated with heparin. Wash with isotonic PBS buffer (2000r / min, 5min) 3 times. Put the washed red blood cells (20-fold dilution) on the formed discontinuous density gradient solution, centrifuge at 2700r / min for 30min, take the red blood cells at the bottom layer (ie old red blood cells), wash them three times with PBS (2000r / min, 5min) Suspended in isotonic PBS buffer and stored at 4°C for later use.

[0041] (2) Use sialic acid to act on aged red blood cells. Take 0.1ml of the washed aged red blood cells prepared in (1), dilute to 1ml with 1640 solution containing 10% calf serum by volume, the concentration is about 5×10 8 pieces / ml. Then add 50 μl of sialic acid working solution with a concentration of 40 μg / ml (that is, an aqueous solution of sialic acid with a concentration of 40 μg / ml), a...

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Abstract

The invention discloses a biologic technology method of restoring the structure and the function of RBC, mainly uses antioxidant or acylneuraminate to act on the aged or pathological RBC to obtain RBC, the structure and the function of which are all restored to the level of normal younger RBC. The method of the invention has simple steps and convenient and safe operation, can restore the surface electric charges of the aged or pathological RBC to the level of younger RBC of normal youth, and then restores the structure and the function without bad influence on the RBC.

Description

technical field [0001] The invention belongs to the technical field of cell biology, in particular to a biotechnological method for restoring the structure and function of red blood cells. Background technique [0002] The main function of red blood cells is to transport oxygen and carbon dioxide, and the realization of this function must rely on their normal cell morphology and rheological properties. The relevant characteristics of the surface of the red blood cell membrane, including the surface morphology of the cell, the charging characteristics of the cell surface, etc., not only directly affect the adhesion, aggregation, metabolism and immunity of the cell, but also affect the deformability of the red blood cell and its flow in the capillary , thereby affecting the function of red blood cells to transport oxygen and other nutrients, and affecting the overall characteristics of blood. Relevant studies have shown that the surface morphology of cells and the mechanical ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N5/08C12N5/078
Inventor 黄耀熊
Owner JINAN UNIVERSITY
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