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Agent for activation sector cruor activating-enzyme time (APTT)

A technology of thromboplastin time and reagents, which is applied in the field of reagents for determining activated partial thromboplastin time in plasma, and can solve the problems of prolonged clot time, long activation time of endogenous blood clots, and low sensitivity of heparin

Inactive Publication Date: 2008-07-23
CHANGAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Ellagic acid and its derivatives are used as liquid active materials, which have low sensitivity to heparin
As a solid activation material such as Gaocen earth, diatomaceous earth, etc., its disadvantage is that the activation time of endogenous blood clots is long and requires a long incubation period
Moreover, these solid active materials are all natural mineral materials, and the consistency and uniformity of their quality may affect the measurement results. In addition, they are required to exist in a suspended state. If precipitation occurs, it will prolong the caking time and cause blood coagulation. An error occurred at the termination point of the meter determination

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Embodiment 1: Weigh 1.15g N-2-hydroxyethylpiperazine-N'-2-ethanesulfonic acid and 2.25g glycine in 400mL sterile distilled water, stir and dissolve, measure the pH at 7.30 with a pH meter, otherwise Adjust to 7.30 with dilute sodium hydroxide or dilute hydrochloric acid, add water to 700mL, add 0.12g of nano silicon dioxide, stir and mix evenly on a magnetic stirrer, add 100mg of rabbit cephalin, stir for 30min, mix well, then add 0.1m mol.L -1 MgCl 2 20mL, 0.1g of 1% bovine serum albumin, 3.0g of polyethylene glycol, stirred, left for more than 4 hours is a liquid test APTT reagent, in order to facilitate storage and transportation, 5g of rejuvenating agent mannitol can be added, and freeze-dried for storage.

Embodiment 2

[0022] Embodiment 2: Take by weighing 1.0mL of 2.0g Tris and 10% hydrochloric acid in 400mL sterile distilled water, stir and dissolve, measure the pH at 7.30 with a pH meter, otherwise adjust to 7.30, add water to 700mL, add 0.5g of nano silicon dioxide, stir and mix evenly on a magnetic stirrer, add 200mg bovine cephalin, stir for 30min, mix well, then add 0.5mmol.L -1 MgSO 4 20mL, 0.5g of 1% bovine serum albumin, 3.0g of polyethylene glycol, stirring, and standing for more than 4 hours is the liquid test APTT reagent. In order to facilitate storage and transportation, 4.0g of rejuvenating agent trehalose can be added, and freeze-dried for storage.

Embodiment 3

[0024] Weigh 0.05g of N-2-hydroxyethylpiperazine-N'-2-ethanesulfonic acid and 0.1g of glycine in 400mL of sterile distilled water, stir to dissolve, measure the pH at 7.30 with a pH meter, otherwise oxidize with dilute hydrogen Adjust sodium or dilute hydrochloric acid to 7.30, add water to 700mL, add 0.05g of nano-silica with different characteristics, stir and mix evenly on a magnetic stirrer, add 20mg rabbit cephalin, stir for 30min, mix well, and then add 0.5mmol. L -1 MnSO 4 20mL, 0.3g of 1% bovine serum hemoglobin, 0.1g of polyethylene glycol, stirred, and left for more than 4 hours is the liquid test APTT reagent.

[0025] The influence of nano-silica with different characteristics on APTT is as follows:

[0026] 1. The influence of silica hydrophilicity on APTT time

[0027] During the processing and manufacturing process of nano-silica, silica has been modified differently, which can be divided into hydrophobicity and hydrophilicity. The nano silicon dioxide invol...

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Abstract

The invention relates to a detection agent for hemostasis and thrombus belonging to medical clinical detection technical field, in particular to a detection agent for measuring activation partial thromboplastin time (APTT) of blood plasma. The invention is characterized in that the agent is prepared from 0.05-0.5g / L agent solid activator, 20-200mg / L phosphatide, 0.1-10mmol / L divalent metal ion salt, 0.1-0.5g / L stabilizer, 0.1-10g / L polyethylene glycol PEG, 0.05-4.0g / L buffer agent, 2.0-5.0g / L revival agent, while the left is water. The invention provides a high-stability standard agent for meeting APTT measurement, with simple operation, suitable price, short activation time and high sensitivity.

Description

technical field [0001] The invention relates to a detection reagent for hemostasis and thrombus in medical clinical examination, in particular to a reagent for measuring activated partial thromboplastin time (APTT) in blood plasma. Background technique [0002] In terms of clinical testing, the detection of hemostasis and thrombus in clinical laboratories is widely used in the diagnosis of bleeding, thrombotic diseases and prethrombotic conditions, efficacy observation, anticoagulant dose monitoring and prognosis analysis. With the development of science and technology and the advancement of basic medical research, people have a deeper understanding of the occurrence and development of hemostasis and thrombus, and their detection methods have become more and more advanced. Wide application in hemostasis detection. [0003] The activated partial thromboplastin time (APTT) of a plasma sample can be used to determine whether the intrinsic coagulation activity mechanism is in a...

Claims

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Application Information

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IPC IPC(8): G01N33/86
Inventor 杨胜科费晓华
Owner CHANGAN UNIV
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