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Three-dimensional microcosmic appearance inclined scanning method and apparatus

A technology of micro-morphology and oblique scanning, which is applied in the field of 3D scanning, can solve the problems of low efficiency, time-wasting, inability to move laterally, etc., and achieve the effect of improving scanning speed and efficiency and reducing scanning time

Inactive Publication Date: 2008-07-23
武汉交通科技研究院有限责任公司
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Problems solved by technology

However, these methods scan an object longitudinally and vertically to obtain a three-dimensional image of only one area. During the scanning process, horizontal movement cannot be performed. If you want to obtain a larger three-dimensional image, you need to re-move a place and re-vertical Vertical scanning, and then splicing to get a larger three-dimensional image, most of the time in this process is to scan the unfocused surface, which is a waste of time and low efficiency

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  • Three-dimensional microcosmic appearance inclined scanning method and apparatus
  • Three-dimensional microcosmic appearance inclined scanning method and apparatus
  • Three-dimensional microcosmic appearance inclined scanning method and apparatus

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Embodiment Construction

[0021] The present invention adopts the oblique scanning method of three-dimensional microscopic topography, that is, the objective lens of the microscope is moved along the oblique loading platform, and the spatial displacement of the loading platform is controlled, and the displacement signal is transmitted to the computer through a displacement recorder (such as a photoelectric encoder) Among them, the coordinates P, L, and H of the loading platform are obtained, and P, L, and H are respectively the X, Y, and Z coordinates of the origin O coordinate of the first image that the microscope loading platform begins to measure; Plane scanning, the microscopic image of the sample is obtained through the microscope and the camera, and the images of the focal plane in the horizontal and vertical directions are obtained, and then the focal plane range image of the three-dimensional topography is obtained through image processing, and the three-dimensional topography of the object is s...

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Abstract

The invention relates to a three-dimensional micro-morphology oblique scanning method and a corresponding device. The method is characterized in that the position of a focus object is adjusted by three stepping motors controlled by a computer, ensuring the object always at the central place in the oblique scanning and obtaining image texture and high-level information of the focus surface of the object, then obtaining a lager three-dimensional morphology image through synthesis and restructure. The device comprises the computer, a microscope and a camera (1); wherein, the camera is arranged on the microscope, an object-carrying platform (13) of the microscope is provided with an object-carrying wedge-shaped platform (14), the object-carrying wedge-shaped platform and a drift angle of the object-carrying platform forms a oblique focus surface when the drift angle Beta is 5 to 40 degrees, the three-dimensional movement space coordinate of the object and the image of the camera at the space coordinate are inputted to the computer at the same time, then restructuring the three-dimensional micro-morphology high-level and texture information on the basis. The three-dimensional micro-morphology oblique scanning method and a corresponding device has the advantages of avoiding useless work in non-focus area, reducing scanning time and improving scanning speed and efficiency.

Description

technical field [0001] The invention relates to three-dimensional scanning, in particular to a method and device for oblique scanning of three-dimensional microscopic topography. Background technique [0002] At present, the principle of focal plane can be used to solve the three-dimensional image processing and reconstruction of microscopic cells, and the method of deconvolution and blind deconvolution is used to process the three-dimensional image of the fluorescence of biological cells by using a fluorescence microscope to improve the resolution of the image [2], Using this principle to design patents "ZL 2005200967777.9 (Notice No. CN2804794)" and "200510018921.1 (Publication No. CN1715987)", these can record three-dimensional surface information in the form of two-dimensional plane image sequences and one-dimensional height data. By analyzing these information Reconstruction can obtain intuitive three-dimensional surface information, and then use the three-dimensional s...

Claims

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Application Information

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IPC IPC(8): G01B11/24G06T17/00
Inventor 吕植勇严新平彭雅芳
Owner 武汉交通科技研究院有限责任公司
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