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Nano fibrous tissue engineering blood vessel and preparation thereof

A nanofiber, tissue engineering technology, applied in the field of biomedical applications of polymer materials, to prevent embolism, firm and uniform binding, and prevent blood penetration

Inactive Publication Date: 2008-07-09
SUZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, using the good complementarity of the two materials, the combination of natural materials and synthetic materials to form composite nanofiber tissue engineering blood vessels with good mechanical and biological properties and its preparation methods have not been reported so far.

Method used

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  • Nano fibrous tissue engineering blood vessel and preparation thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] 1. Put 0.2kg of waste mulberry silk into 6L of 0.05% sodium carbonate aqueous solution, boil for 0.5h, repeat the boiling three times, remove all the sericin on the surface of the silk, and dry it at room temperature to obtain silk fibroin;

[0027] 2. Dissolve the dried silk fibroin with 1.2L calcium chloride, water and ethanol solution with a molar ratio of 1:8:2 at 75±2°C to form a silk fibroin solution;

[0028] 3. Pour the silk fibroin solution prepared in step 2 into a cellulose dialysis bag, first dialyze with tap water for 48 hours, then dialyze with deionized water for 8 hours, remove ethanol and small calcium chloride molecules in the solution, and filter to obtain Pure silk fibroin solution;

[0029] 4. Take 100ml of pure silk fibroin solution and pour it into an area of ​​20×20cm 2 In a polystyrene plastic tray, dry and form a film at constant temperature and humidity (25°C, RH65%);

[0030] 5. Weigh 6.58g of regenerated silk fibroin film and 3.49g of medi...

Embodiment 2

[0036] 1. Put 0.1kg of waste silk into 3L of 0.05% sodium carbonate aqueous solution, boil for 0.5h, repeat the treatment three times, remove all the sericin around the silk, and obtain silk fibroin;

[0037] 2. Dissolve the dried silk fibroin obtained in step 1 with 0.6 L of calcium chloride, water, and ethanol solution with a molar ratio of 1:8:2 at 78±2° C. to form a silk fibroin solution;

[0038] 3. Pour the above-mentioned silk fibroin solution into a cellulose dialysis bag, dialyze with tap water first, and then dialyze with deionized water to remove ethanol and small calcium chloride molecules in the solution, and then filter with multi-layer degreasing gauze to prepare Obtain pure silk fibroin solution;

[0039] 4. Take 100ml of pure silk fibroin solution and pour it into an area of ​​20×20cm 2 In a stainless steel plate, freeze at -20°C for 8 hours, and then vacuum-dry in a freeze dryer for 20 hours to obtain a spongy regenerated silk fibroin film;

[0040] 5. Weig...

Embodiment 3

[0046] 1. Put 0.15kg of waste silk into 4.5L of 0.05% sodium carbonate aqueous solution, boil for 0.5h, and repeat the treatment three times to obtain silk fibroin;

[0047] 2. Dissolve the dried silk fibroin obtained in step 1 with 0.9 L of calcium chloride, water, and ethanol solution with a molar ratio of 1:8:2 at 75±2° C. to form a silk fibroin solution;

[0048] 3. Pour the above-mentioned silk fibroin solution into a cellulose dialysis bag, dialyze with tap water first, and then dialyze with deionized water to remove ethanol and small calcium chloride molecules in the solution, and then filter with multi-layer degreasing gauze to prepare Obtain pure silk fibroin solution;

[0049] 4. Take 100ml of pure silk fibroin solution and pour it into an area of ​​20×20cm 2 In the ABS dish of , dry at room temperature to obtain regenerated silk fibroin film;

[0050] 5. Weigh 6.7g of regenerated silk fibroin film, 1.3g of gelatin and 0.7g of elastin, add to 70.5g of hexafluoroiso...

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Abstract

The invention relates to a tissue engineering material and a preparation method thereof, in particular to a nano fiber tissue engineering blood vessel and a preparation method thereof. The invention consists of a three-dimensional reticular non-woven film formed by an inner layer of nano fiber and an outer layer of nano fiber; the inner layer of the blood vessel is natural polymer, wherein, calculated by weight, 40 percent to 80 percent is fibroin, 20 percent to 50 percent is gelatine, 0 percent to 20 percent is extracellular matrix protein; while the outer layer of the blood vessel is synthetic polymer. The preparation method is that the natural polymer is dissolved in trifluroroethyl and other solution, while the synthetic polymer is dissolved in hexafluoroisopropanol and other solution, which are respectively prepared into spinning solution; the static electricity spinning technique is adopted to subsequently form the inner and the outer layers on a gather roller; cross-linked treatment is conducted after the inner and the outer layers are taken down, to prepare the nano fiber tissue engineering vessel. The inner layer can simulate the structure of the extracellular matrix, provide good environment for endothelial cells to grow, support adhesion, proliferation and differentiation of the cells, and is good for endothelization of the blood vessel; and the outer layer has good mechanical performance.

Description

technical field [0001] The invention relates to a tissue engineering material and a preparation method thereof, in particular to a nanofibrous membrane (inner layer of a blood vessel) formed by blending silk fibroin, gelatin and extracellular matrix protein, and then combined with a synthetic polymer fiber membrane (outside of a blood vessel) The invention relates to a method for constructing tissue engineered blood vessels by compound layering, belonging to the biomedical application field of polymer materials. Background technique [0002] Vascular injury has become a difficult problem in the medical field due to its poor self-repair ability. Since Voorhees successfully developed Vinylon artificial blood vessel for the first time in 1952, and it was successfully used clinically in the following year, people have successively developed artificial blood vessels with various materials and various molding methods. The commonly used graft blood vessels are mainly woven with no...

Claims

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Application Information

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IPC IPC(8): A61L27/40A61L27/58A61L27/26A61F2/06D01F8/02
Inventor 尹桂波张幼珠王红卫王曙东王立新吴佳林施德兵董智慧符伟国
Owner SUZHOU UNIV
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