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Thyroxine chemiluminescence immune analysis quantitative measuring reagent kit and method for preparing the same

A technology for quantitative determination of thyroxine, applied in the field of immunodiagnosis, can solve the problems of high price, complicated operation, and difficult to popularize.

Active Publication Date: 2008-06-18
CHEMCLIN DIAGNOSTICS CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, these foreign automatic chemical photoenzyme immunoassay analyzers and matching kits are mostly used in conjunction with closed systems, which are expensive and complicated to operate, and are not easy to promote in China

Method used

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  • Thyroxine chemiluminescence immune analysis quantitative measuring reagent kit and method for preparing the same
  • Thyroxine chemiluminescence immune analysis quantitative measuring reagent kit and method for preparing the same
  • Thyroxine chemiluminescence immune analysis quantitative measuring reagent kit and method for preparing the same

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Embodiment Construction

[0069] 1. Preparation of chemiluminescence substrate solution:

[0070] Solution A: Add 1.21g Tris and 295μL concentrated HCl to 100ml double distilled water to make 0.1M Tris-HCl buffer solution with pH8.5. Add 0.5 g of Luminol, 20 μL of Tween20, and 0.1 g of benzofluoranthene (the structure is shown in the figure below) to this buffer, and mix well.

[0071] Solution B: Add 0.73 g of trisodium citrate and 0.44 g of citric acid into 100 ml of double-distilled water to prepare a citric acid buffer solution of 0.1M pH4.6, and add 100 μL of 30% hydrogen peroxide solution to this solution.

[0072] How to use: Mix liquid A and liquid B at a ratio of 1:1 before use.

[0073]

[0074] 2. Preparation of enzyme markers:

[0075] 2.1 Biotinylated T4

[0076] (1) Connection between T4 and BSA

[0077] Take 1 mg of T4 whose carboxyl group has been methylated and dissolve it in 0.2 mL DMF to form a 5 mg / mL solution, add 1 mL of BSA with a concentration of 0.5 mg / mL (dissolved in 0...

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Abstract

The invention belongs to the technical field of immunodiagnosis, and discloses a thyroxine (T4) enzymatic chemiluminescence quantitative determination kit. The kit disclosed by the invention consists of a T4 standard product, an antibody pre-coated reaction plate, a horseradish peroxidase marker, diluent, cleaning mixture, and chemiluminescence substrate liquid. The kit disclosed by the invention has the advantages of quickness, handiness, sensitiveness, cheapness and high repeatability, and is an important index for diagnosis and therapeutic evaluation of hyperthyroidism and hypothyroidism. The invention has high clinic use value. The invention also provides a method to prepare the kit.

Description

technical field [0001] The invention belongs to the technical field of immunodiagnosis, and in particular relates to a thyroxine (T4) quantitative determination kit (chemiluminescence method), a preparation method and a method for using the same to measure thyroxine. Background technique [0002] Thyroxine, also known as tetraiodothyronine (T4), is formed by the thyroid gland actively capturing inorganic iodine and combining it with tyrosine. The molecular weight is 777. T in circulating blood 1 / 2 6 to 7 days. 99.96% bound to binding protein in peripheral blood. T4 forms anti-T3 and T3 in peripheral tissues through the action of 5-deiodinase and 5'-deiodinase, respectively. Thyroid hormone plays an important role in body function, so the detection of thyroxine can reflect whether the thyroid function is normal. An increase is more common in hyperthyroidism, and a decrease is more common in hypothyroidism. [0003] Thyroxine (T4) content is too high (hyperthyroidism) or t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/543G01N33/531G01N21/76
Inventor 林斯胡国茂应希堂
Owner CHEMCLIN DIAGNOSTICS CO LTD
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